We narrowed to 14,933 results for: pET

1. pET22b-ecDHFR-N23PP/L28F/G51PEKN

   Plasmid

   #118585

   Purpose

   Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

   Insert

   DHFR
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N23 mutated to P23, L28 mutated to F28, G51 mutat…

   Promoter

   T7

   Available since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a SUMO Nup53xl 162-267

   Plasmid

   #86002

   Purpose

   bacterial expression of his6 and SUMO tagged RRM motif of Xenopus laevis Nup53

   Depositor

   Wolfram Antonin

   Type

   Empty backbone

   Use

   Tags

   SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. HIV-1 integrase W235F in pET-15b

   Plasmid

   #61681

   Purpose

   HIV-1 integrase W235F in pET-15b

   Depositor

   Robert Craigie

   Article

   Li et al EMBO J. 2006 Mar 22;25(6):1295-304. Epub

   Insert

   HIV-1 integrase W235F
   

   Use

   Tags

   His

   Expression

   Mutation

   W235F

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

4. HOXA9 (murine) HIS-tag pET

   Plasmid

   #8492

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HoxA9 (Hoxa9 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

5. HOXB3 (human) HIS-tag pET

   Plasmid

   #8524

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB3 (HOXB3 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Contains 5 additional amino acids before ATG from…

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. HOXC8 (murine) HIS-tag pET

   Plasmid

   #8567

   Depositor

   Corey Largman

   Insert

   HOXC8 (Hoxc8 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. HOXC6 (human) HIS-tag pET

   Plasmid

   #8565

   Depositor

   Corey Largman

   Insert

   HOXC6 (HOXC6 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   This protein starts at an internal Met initially …

   Promoter

   Available since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156)

   Plasmid

   #61939

   Purpose

   Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF1) (UCHL5 Human)
   hNFRKB (1-156)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 156

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

   Plasmid

   #61938

   Purpose

   Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

   Inserts

   hUCH37 (ISF2) (UCHL5 Human)
   hNFRKB (39-156) (NFRKB Human)
   

   Use

   Tags

   6x HIS

   Expression

   Bacterial

   Mutation

   Construct ends at residue 156, residues 2-38 dele…

   Promoter

   T7

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pET30a-ASXL1-HARE-HTH domain

    Plasmid

    #107164

    Purpose

    Expresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coli

    Depositor

    Douglas Feldman

    Article

    Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

    Insert

    ASXL1 (Asxl1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Contains amino acids 2-83

    Promoter

    T7

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. delNC C-His Ana GvpC in pET28a

    Plasmid

    #85736

    Purpose

    Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (truncated and his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Deletion of N and C terminal regions, addition of…

    Promoter

    T7

    Available since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET151-GFP-PP-RPN2 (925-953)

    Plasmid

    #73744

    Purpose

    Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Use

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 932-953 of RPN2

    Promoter

    T7

    Available since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pET51b-His-TEV-SNAP-tag_cx

    Plasmid

    #167273

    Purpose

    Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    P179R

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b/GgVcl 884-1066 (aka Vt)

    Plasmid

    #46176

    Depositor

    Susan Craig

    Article

    Steimle et al J Biol Chem. 1999 Jun 25;274(26):18414-2

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-ybbR-HIS-CBM-CohI

    Plasmid

    #58709

    Purpose

    Probing molecule to perform single molecule force spectroscopy experiments

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    CBM-CohI
    

    Use

    Tags

    HIS, HRV 3C, and ybbR

    Expression

    Bacterial

    Mutation

    changed Alanine 2 to Cysteine in CBM domain

    Promoter

    T7

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. HOXB13 (human) HIS-tag pET

    Plasmid

    #8543

    Depositor

    Corey Largman

    Article

    Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

    Insert

    HOXB13 (HOXB13 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western and by transcription/tra…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. HOXD12 (murine) HIS-tag pET

    Plasmid

    #8553

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXD12 (Hoxd12 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Fusion sequence is: ggatccATG

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. p139 pETF-GST RanBP10 L301I

    Plasmid

    #11092

    Depositor

    Ramesh Shivdasani

    Insert

    RanBP10 (Ranbp10 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    L301I; see depositor comments below

    Promoter

    Available since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. HOXD4 (murine) HIS-tag pET

    Plasmid

    #8548

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXD4 (Hoxd4 Mouse)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    makes protein by Western and by in vitro transcri…

    Promoter

    Available since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only