We narrowed to 15,158 results for: pET

1. pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

   Plasmid

   #167268

   Purpose

   Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HOB
   

   Tags

   His x10

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET29-hMIGA2(170-593)-6xhis

   Plasmid

   #192834

   Purpose

   Express codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteria

   Depositor

   Karin Reinisch

   Article

   Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

   Insert

   Codon-optimized human Mitoguardin 2 (MIGA2 Human)
   

   Tags

   6xhis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pF712-pET28a-Hs-delta44-GCDH-NHis wt

   Plasmid

   #85113

   Purpose

   expression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tag

   Depositor

   Pal Falnes

   Article

   Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

   Insert

   human mature (delta44) GCDH (GCDH Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   silent SNP: G1041T

   Promoter

   T7

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-His6-Sumo-IntNtr-eSrt2A

   Plasmid

   #126015

   Purpose

   Bacterial expression of IntNtr-eSrt2A fusion protein (with N-terminal His6-Sumo)

   Depositor

   Thomas Muir

   Insert

   His6-Sumo-IntNtr-eSrt2A
   

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. HOXA10 (human) HIS-tag pET

   Plasmid

   #8516

   Depositor

   Corey Largman

   Article

   Lowney et al Nucleic Acids Res 1991 Jun 25;19(12):344

   Insert

   HOXA10 (HOXA10 Human)
   

   Expression

   Bacterial

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pET15b-Casp8 delta DED C360A

   Plasmid

   #11828

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 8 minus Death Effecter Domain catalytic mutant (CASP8 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Minus Death Defecter Domain (aa 1-216), C360A

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

7. HOXA5 (human) HIS-tag pET

   Plasmid

   #8562

   Depositor

   Corey Largman

   Insert

   HOXA5 (HOXA5 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   SacI site at amino acids 1-2 was used to clone in…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pET28a-RBP-GFP(S45C)-ZdK1

   Plasmid

   #185791

   Purpose

   Encodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linker

   Depositor

   Lizhong He

   Article

   Younas et al J Phys Chem Lett. 2020 Oct 15;11(20):864

   Insert

   RBP-sfGFP(S45C)-ZdK1
   

   Tags

   10xHis, TEV protease cleavage site after His Tag

   Expression

   Bacterial

   Mutation

   Ser to Cys mutation at start of GFP, residue 45 i…

   Promoter

   T7

   Available Since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXC9 (murine) HIS-tag pET

   Plasmid

   #8559

   Depositor

   Corey Largman

   Insert

   HOXC9 (Hoxc9 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET-45b-mEGFP-HMGB1-K184Rfs

    Plasmid

    #194544

    Purpose

    Bacterial Expression of 6xHis mEGFP-HMGB1 Mutant (K184Rfs*44) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    HMGB1 (HMGB1 Human)
    

    Tags

    mEGFP

    Expression

    Bacterial

    Mutation

    K184Rfs*44

    Available Since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEW4: pET-32a Dot1L(2-386)

    Plasmid

    #124099

    Purpose

    Dot1L(2-386) expression plasmid

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Cell. 2019 Feb 8. pii: S0092-8674(19)301

    Insert

    Dot1L(2-386) (DOT1L Human)
    

    Tags

    Trx-His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-28a+GFP-CAMSAP2 CKK

    Plasmid

    #59032

    Purpose

    expresses CKK domain of CAMSAP2 in E. coli

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CKK domain (CAMSAP2 Human)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Available Since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pET-FLAG-SpCas9-HF1-plus

    Plasmid

    #126775

    Purpose

    Expression of increased fidelity SpCas9-HF1-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as SpCas9-HF1.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    N497A; Q695A; Q926A; amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21c-His-Xa-Gluc-Xa-C9D-TGinsert

    Plasmid

    #124660

    Purpose

    reporter protein which emits bright blue fluorescence

    Depositor

    Yutaka Kuroda

    Article

    Wu et al Sci Rep. 2020 Nov 18;10(1):20069. doi: 1

    Insert

    Gaussia luciferase-C9D-TGinsert
    

    Tags

    IEGRTMGDDDGDDDGDDD（FactorXa,TMG,C9D-Tag） and MHHH…

    Expression

    Bacterial

    Mutation

    changed glutamic acid 100 to alanine, and glycine…

    Promoter

    T7 promoter

    Available Since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-His8-aCat(660-906)

    Plasmid

    #203333

    Purpose

    Bacterial expression of alpha-catenin F-actin binding domain

    Depositor

    Tina Izard

    Article

    Rangarajan et al J Biol Chem. 2023 Feb;299(2):102817. doi

    Insert

    CTNNA1 (CTNNA1 Human)
    

    Tags

    8xHis with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    aa 660-906 only

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-Duet1-6xHis-TEV-LC3BGlydelta5C

    Plasmid

    #190237

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-LC3BGlydelta5C. Internal reference: SMC893.

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    MAP1LC3B (MAP1LC3B Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HOXA11 (murine) HIS-tag pET

    Plasmid

    #8517

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

    Insert

    HOXA11 (Hoxa11 Mouse)
    

    Tags

    T7

    Expression

    Bacterial

    Mutation

    makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

19. HOXA2 (murine) HIS-tag pET

    Plasmid

    #8557

    Depositor

    Corey Largman

    Insert

    HOXA2 (Hoxa2 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    contains 5 additional amino acids from 5' un…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

20. HOXB2 (human) HIS-tag pET

    Plasmid

    #8522

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB2 (HOXB2 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at AA #2; makes protein by Western

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only