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pGCS-C2m(3xHA)

Plasmid

#85725

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGCS-C6(eGFP)

Plasmid

#85729

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

EGFP

Available Since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

Plasmid

#154088

Purpose

Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-LCOR-P2A-HOXA9-T2A-HOXA5-IMPROVED

Plasmid

#154088

Purpose

Improved version to express LCOR, P2A, HOXA9, T2A and HOXA5 in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Ligand Dependent Nuclear Receptor Corepressor, HOXA9, HOXA5 (LCOR, HOXA9, HOXA5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF2

Plasmid

#141189

Purpose

Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF2 (GIGYF2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/GFP-GIGYF2

Plasmid

#141189

Purpose

Expresses GFP-GIGYF2 in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

GIGYF2 (GIGYF2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B-GFP

Plasmid

#123107

Purpose

Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B-GFP

Plasmid

#123107

Purpose

Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 WT

Plasmid

#123103

Purpose

Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 WT

Plasmid

#123103

Purpose

Expresses 3xFLAG-GABARAPL2 wild-type in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CCND1

Plasmid

#116721

Purpose

Lentiviral expression of CCND1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-CCND1

Plasmid

#116721

Purpose

Lentiviral expression of CCND1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-CA-CMV-GFP

Plasmid

#83255

Purpose

Adenoviral expression of constitutively active STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

constitutively active

Promoter

CMV

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAD-CMV-STAT5A-CA-CMV-GFP

Plasmid

#83255

Purpose

Adenoviral expression of constitutively active STAT5A with co-expression of GFP

Depositor

Andrew Brooks

Article

Legrand et al J Invest Dermatol. 2016 Sep;136(9):1781-

Inserts

Stat5a (Stat5a Mouse)
eGFP

Use

Adenoviral

Mutation

constitutively active

Promoter

CMV

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pLV CMV-TERRA

Plasmid

#111375

Purpose

TERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA polyA

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLV CMV-TERRA

Plasmid

#111375

Purpose

TERRA expression contains approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Article

Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

Insert

TERRA polyA

Use

Lentiviral

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-FRT/TO-3xFLAG-REV7

Plasmid

#114127

Purpose

Expresses N-terminally tagged 3x FLAG-REV7 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

REV7 (MAD2L2 Human)

Use

Genomic integration, tetracycline inducible

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV/TetO2

Available Since

Aug. 16, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP‐RNASEH2C

Plasmid

#108698

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2C (RNASEH2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2C

Plasmid

#108698

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2C (RNASEH2C Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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