We narrowed to 5,308 results for: pcDNA3.1+

1. pER-eZinCh-2

   Plasmid

   #70009

   Purpose

   expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

   Depositor

   Maarten Merkx

   Article

   Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

   Insert

   ER-eZinCh-2
   

   Use

   Tags

   KDEL sequence and PPI signal

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Dr-EGFR

   Plasmid

   #184004

   Purpose

   Dr-EGFR expression in mammalian cells

   Depositor

   Vladislav Verkhusha

   Article

   Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

   Insert

   Dr-EGFR (EGFR Human)
   

   Use

   Tags

   Deinococcus radiodurans bacterial phytochrome

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FKBP-CD28TMD-NTEVp(H75S)

   Plasmid

   #137828

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75S)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Serine in NTEVp fragment

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmyc-GFP-TNRC6A-NES-mut

   Plasmid

   #42001

   Depositor

   Kumiko Ui-Tei

   Article

   Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   EGFP and Myc

   Expression

   Mammalian

   Mutation

   F951A, F955A, I958A, F960A

   Promoter

   CMV

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. FKBP-CD28TMD-NTEVp(WT)

   Plasmid

   #137826

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(WT)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FLAG-YL1

   Plasmid

   #15333

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

   Insert

   transcription factor-like 1 (VPS72 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA_DiCre_60.F2

   Plasmid

   #60208

   Purpose

   Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

   Depositor

   Jean-Paul Herman

   Article

   Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

   Insert

   Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
   

   Use

   Tags

   FRAP and Nuclear localisation signal

   Expression

   Mammalian

   Mutation

   amino acids 2021-2112 of FRAP with a T2098L mutat…

   Promoter

   CMV

   Available since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-NES-ZapCmR2

   Plasmid

   #59015

   Purpose

   Cytosolic zinc-binding FRET biosensor

   Depositor

   Amy Palmer

   Article

   Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

   Insert

   NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
   

   Use

   Tags

   Nuclear Exclusion Signal

   Expression

   Mammalian

   Mutation

   See comment

   Promoter

   CMV

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

9. psgRNA2.02-PP7-ccdB

   Plasmid

   #99912

   Purpose

   Basic cloning vector for the expression of PP7 inserted sgRNA

   Depositor

   Yujie Sun

   Article

   Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mGFAP-Halo

    Plasmid

    #169474

    Purpose

    the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

    Depositor

    Federico Herrera

    Article

    Letra-Vilela et al Experimental Results (2020), Volume 1, e

    Insert

    GFAP (Gfap Mouse)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Changed R 236 to H

    Promoter

    Available since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP-CMV-jGCaMP8f F366H

    Plasmid

    #204141

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f F366H
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Phenylalanine 366 changed to histidine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. psgRNA2.02-MS2-ccdB

    Plasmid

    #99911

    Purpose

    Basic cloning vector for the expression of MS2 inserted sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al Nucleic Acids Res. 2016 May 19;44(9):e86

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Bongwoori

    Plasmid

    #63720

    Purpose

    Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

    Depositor

    Bradley Baker

    Article

    Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

    Insert

    Bongwoori
    

    Use

    Tags

    Super ecliptic pHlorin A227D

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

    Plasmid

    #188993

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    D201G, A258T, K327R, G328S, G381V, G429D, T450A

    Promoter

    CMV

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pmyc-GFP-TNRC6A-NLS-mut

    Plasmid

    #42000

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A

    Promoter

    CMV

    Available since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSSA-PMG (pXK-1649)

    Plasmid

    #208857

    Purpose

    SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCDNA-SCW-HHL

    Plasmid

    #190634

    Purpose

    Mammalian expression of genetically encoded calcium indicator SCW-HHL

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SCW-HHL
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PA-RL-mCherry

    Plasmid

    #78861

    Purpose

    DULIP negative control (bait) to exclude unspecific background binding

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    mCherry
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-dMASS_1

    Plasmid

    #209764

    Purpose

    Expression of dMASS_1 in mammalian cells under CMV promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-HA-CAML

    Plasmid

    #22284

    Depositor

    Erik Lillehoj

    Article

    Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

    Insert

    calcium-modulating ligand (CAMLG Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2009

    Availability

    Academic Institutions and Nonprofits only