We narrowed to 9,744 results for: try

1. saposin B^W

   Plasmid

   #161759

   Purpose

   Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

   Depositor

   Sam Gellman

   Article

   Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

   Insert

   saposin B R38_W
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   changed Arginine 38 to Tryptophan

   Promoter

   T7

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSF-G1(6/7FTrp)RS

   Plasmid

   #207621

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSF-G1(4/5FTrp)RS

   Plasmid

   #207620

   Purpose

   tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

   Depositor

   Thomas Huber

   Article

   Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

   Insert

   tRNA synthetase
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-21(+)Tm9D8* TrpB

   Plasmid

   #207517

   Purpose

   Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

   Depositor

   Thomas Huber

   Article

   Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

   Insert

   Tryptophan synthase β-subunit
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28-MBP-CI2F

   Plasmid

   #191254

   Purpose

   Expresses chymotrypsin inhibitor with an N-terminal MBP fusion protein

   Depositor

   Arthur Laganowsky

   Article

   Raab et al J Am Chem Soc. 2020 Oct 14;142(41):17372

   Insert

   Chymotrypsin Inhibitor 2
   

   Tags

   Maltose Binding Protein

   Expression

   Bacterial

   Available Since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEPQD1CB0808

   Plasmid

   #177082

   Purpose

   MoClo Level 1, position 4, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus promoter regions contains 4x attB sites for recruitment fo gal4AD-phiC31

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   tryptophan decarboxylase (CrTDC) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEPQD1CB0774

   Plasmid

   #177064

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   tryptophan decarboxylase (CrTDC) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEPQD1CB0773

   Plasmid

   #177063

   Purpose

   MoClo Level 1, position 1, transcriptional unit for transient expression of tryptophan decarboxylase (CrTDC) from Catharanthus roseus driven by 35S promoter

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   tryptophan decarboxylase (CrTDC) from Catharanthus roseus
   

   Use

   Synthetic Biology

   Available Since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. piggyBAC-H2B FR-MQV

    Plasmid

    #194079

    Purpose

    Expresses nuclear-localized FR-MQV in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

    Insert

    FR-MQV
    

    Tags

    Histone H2B

    Expression

    Mammalian

    Mutation

    Methionine 42 to Glutamine, Cystiene 159 to Valin…

    Promoter

    CMV7

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDule-pCNF

    Plasmid

    #85494

    Purpose

    Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

    Depositor

    Ryan Mehl

    Article

    Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

    Insert

    para-cyanophenylalanine Mj synthetase
    

    Tags

    None

    Expression

    Bacterial

    Mutation

    Y32L L65V F108W Q109M D158G I159P

    Promoter

    lpp (constitutive)

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-UBC-hAAT-IRES-ZsGreen-W

    Plasmid

    #126702

    Purpose

    lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

    Depositor

    Darrell Kotton

    Article

    Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

    Insert

    Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)
    

    Use

    Lentiviral

    Promoter

    UBC

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. tdTomato/pTREX-b

    Plasmid

    #68709

    Purpose

    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Insert

    tdTomato
    

    Use

    Expression of tdtomato red fluorescence protein i…

    Available Since

    Oct. 8, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. H1x

    Plasmid

    #218111

    Purpose

    Encodes mus musculus histone 1 in Gateway entry vector

    Depositor

    Peter Hamilton

    Article

    Kim et al Transl Psychiatry. 2024 Jun 5;14(1):239.

    Insert

    Histone H1x (H1f10 Mouse)
    

    Use

    Gateway cloning entry vector

    Expression

    Bacterial

    Available Since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. KG#797

    Plasmid

    #110916

    Purpose

    Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

    Depositor

    Kenneth Miller

    Article

    Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

    Inserts

    unc-129 promoter
    STRN-1 (Sentryn)-GFP
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    

    Tags

    GFP (contains 3 artificial introns)

    Expression

    Bacterial

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET26b(+)-ATI_QconCAT_1

    Plasmid

    #163957

    Purpose

    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

    Depositor

    Stefan Tenzer

    Article

    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

    Insert

    ATI QconCAT
    

    Tags

    polyhistidine tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUB39

    Plasmid

    #26300

    Depositor

    George Cross

    Insert

    VSG117 (Lister 427-4) cDNA
    

    Use

    Trypanosome inducible expression vector

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-QF2

    Plasmid

    #83307

    Purpose

    codes for QF2 (truncated QF) in a gateway middle entry backbone.

    Depositor

    Marnie Halpern

    Insert

    QF
    

    Available Since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAIP4_2H7W

    Plasmid

    #234220

    Purpose

    Heterologous protein expression of chagasin in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    2H7W (Tc00.1047053506801.80 Trypanosoma cruzi)
    

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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