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pRSETb MGlucoMeter2.6-7m

Plasmid

#247472

Purpose

Matryoshka-based Glucose Sensor

Depositor

Wolf Frommer

Article

Ishikawa et al bioRxiv 2025.09.27.678933; doi: 10.1101/

Insert

Glucose Binding Protein (GBP) with Matryoshka dual fluorophore cassette

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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p5E-2.3k-fabp2

Plasmid

#84927

Purpose

–2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector

Depositor

John Rawls

Insert

–2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)

Tags

NO

Expression

Bacterial

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLEW100cre-EP1

Plasmid

#33345

Purpose

For expression of tetracycline-inducible CRE in Trypanosoma brucei

Depositor

George Cross

Article

Scahill et al Mol Biochem Parasitol. 2008 Jan;157(1):7

Insert

cre recombinase

Use

Cre/Lox; Trypanosoma brucei expression vector

Promoter

TET operator

Available Since

Dec. 1, 2011

Availability

Academic Institutions and Nonprofits only

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saposin B^W

Plasmid

#161759

Purpose

Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

Depositor

Sam Gellman

Article

Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

Insert

saposin B R38_W

Tags

His-tag

Expression

Bacterial

Mutation

changed Arginine 38 to Tryptophan

Promoter

T7

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pRSETb MGlucoMeter2.6-700u

Plasmid

#247470

Purpose

Matryoshka-based Glucose Sensor

Depositor

Wolf Frommer

Article

Ishikawa et al bioRxiv 2025.09.27.678933; doi: 10.1101/

Insert

Glucose Binding Protein (GBP) with Matryoshka dual fluorophore cassette

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pRSETb MGlucoMeter2.6-353n

Plasmid

#247468

Purpose

Matryoshka-based Glucose Sensor

Depositor

Wolf Frommer

Article

Ishikawa et al bioRxiv 2025.09.27.678933; doi: 10.1101/

Insert

Glucose Binding Protein (GBP) with Matryoshka dual fluorophore cassette

Expression

Bacterial

Available Since

Feb. 13, 2026

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6/7FTrp)RS

Plasmid

#207621

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(4/5FTrp)RS

Plasmid

#207620

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 4-Fluoro-L-Tryptophan (4FTrp) and 5-Fluoro-L-Tryptophan (5FTrp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET-21(+)Tm9D8* TrpB

Plasmid

#207517

Purpose

Expression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serine

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

Tryptophan synthase β-subunit

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-BSD-VSG-228

Plasmid

#245116

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-NEO-VSG-228

Plasmid

#245117

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-AnTat1.1-telo

Plasmid

#245103

Purpose

Inserts VSG AnTat1.1 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

AnTat1.1

Use

Other

Mutation

silent G to A mutation at position 471 within AnT…

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pUC19-HYG-BES1-VSG-228-telo

Plasmid

#245104

Purpose

Inserts VSG-228 in T. brucei cells at Lister427 BES1

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pHD309-PUR-VSG-228

Plasmid

#245105

Purpose

Inserts VSG-228 coding sequence in T. brucei tubulin array

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228

Plasmid

#245106

Purpose

Inserts VSG-228 coding sequence in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-177-PUR-VSG-228

Plasmid

#245107

Purpose

Inserts VSG-228 coding sequence in T. brucei minichromosomes using the 177bp repeats

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.noUTRS

Plasmid

#245109

Purpose

Inserts VSG-228 coding sequence without UTRs in T. brucei rDNA spacer

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.500bp

Plasmid

#245110

Purpose

Inserts 500 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:500

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.400bp

Plasmid

#245111

Purpose

Inserts 400 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:400

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pLEW100v5-PUR-VSG-228.300bp

Plasmid

#245112

Purpose

Inserts 300 bp of VSG-228 coding sequence in T. brucei rDNA spacer centered at AnTat1.1 position 694

Depositor

Monica Mugnier

Article

Smith et al Nature (2026)

Insert

VSG-228:300

Use

Other

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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