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High-performance chemical- and light-inducible recombinases in mammalian cells and mice
Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J , Wong WW
Nature Communications (2019) volume 10, Article number: 4845
Article

Chemical-inducible dimerization (CIDs) domains are proteins that can be dimerized through addition of a small-molecule. Moreover, light-inducible dimerization (LIDs) domains also exist that dimerize in response to light. This set of plasmids contains three pairs of heterodimerizing CIDs and one pair of heterodimerizing LIDs. These include the rapalog-responsive FKBP/FRB, gibberellin-responsive GAI/GID1, abscisic acid-responsive PYL/ABI CIDs and blue-light responsive nMag/pMag domains. CIDs/LIDs can be used to recruit proteins with different activities to localize the response. Moreover, CIDs/LIDs can be used to restore the activity of a split protein. This latter strategy was used to develop a large suite of inducible split recombinases.

Supplemental Information

For more information please download the Wong Lab's Cloning Methods to Assemble Split Recombinase Plasmids

WilsonWong_Cloning_Methods.pdf 2.2 MB

Plasmid Pairs for Expressing Chemical and Light-inducible Recombinases

The following plasmid pairs are intended to be used together to produce light or chemically induced recombinase proteins.

Split Protein Induced by N-term Plasmid C-term Plasmid
Cre Recombinase Gibberellin pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpA pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpA
Cre Recombinase Gibberellin pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpA pBW2230_pCAG-PV1-GAI-L1-iCre-252C-NLS-BGHpA
Cre Recombinase Gibberellin pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpA pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpA
Cre Recombinase Gibberellin pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpA pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpA
Cre Recombinase Rapalog pBW2598_pCAG-PV1-iCre-N229-L1-FRB-NLS-BGHpA pBW2606_pCAG-PV1-FKBP-L1-iCre-230C-NLS-BGHpA
Cre Recombinase Rapalog pBW2600_pCAG-PV1-iCre-N251-L1-FRB-NLS-BGHpA pBW2608_pCAG-PV1-FKBP-L1-iCre-252C-NLS-BGHpA
Cre Recombinase Rapalog pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpA pBW2610_pCAG-PV1-FKBP-L1-iCre-257C-NLS-BGHpA
Cre Recombinase Rapalog pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpA pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpA
Cre Recombinase Abscisic Acid pBW2599_pCAG-PV1-iCre-N229-L1-ABI-NLS-BGHpA pBW2607_pCAG-PV1-PYL-L1-iCre-230C-NLS-BGHpA
Cre Recombinase Abscisic Acid pBW2601_pCAG-PV1-iCre-N251-L1-ABI-NLS-BGHpA pBW2609_pCAG-PV1-PYL-L1-iCre-252C-NLS-BGHpA
Cre Recombinase Abscisic Acid pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpA pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpA
Cre Recombinase Abscisic Acid pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpA pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA
Flp Recombinase Gibberellin pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpA
Flp Recombinase Gibberellin pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpA pBW2184_pCAG-PV1-GAI-L1-FlpO-169C-NLS-BGHpA
Flp Recombinase Gibberellin pBW2173_pCAG-PV1-FlpO-N374-L1-GID1-NLS-BGHpA pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA
Flp Recombinase Gibberellin pBW2175_pCAG-PV1-FlpO-N396-L1-GID1-NLS-BGHpA pBW2195_pCAG-PV1-GAI-L1-FlpO-397C-NLS-BGHpA
Flp Recombinase Rapalog pBW2272_pCAG-PV1-FlpO-N27-L1-FRB-NLS-BGHpA pBW2273_pCAG-PV1-FKBP-L1-FlpO-28C-NLS-BGHpA
Flp Recombinase Rapalog pBW2276_pCAG-PV1-FlpO-N168-L1-FRB-NLS-BGHpA pBW2277_pCAG-PV1-FKBP-L1-FlpO-169C-NLS-BGHpA
Flp Recombinase Rapalog pBW2280_pCAG-PV1-FlpO-N374-L1-FRB-NLS-BGHpA pBW2281_pCAG-PV1-FKBP-L1-FlpO-375C-NLS-BGHpA
Flp Recombinase Rapalog pBW2284_pCAG-PV1-FlpO-N396-L1-FRB-NLS-BGHpA pBW2285_pCAG-PV1-FKBP-L1-FlpO-397C-NLS-BGHpA
Flp Recombinase Abscisic Acid pBW2274_pCAG-PV1-FlpO-N27-L1-ABI-NLS-BGHpA pBW2275_pCAG-PV1-PYL-L1-FlpO-28C-NLS-BGHpA
Flp Recombinase Abscisic Acid pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpA pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpA
Flp Recombinase Abscisic Acid pBW2282_pCAG-PV1-FlpO-N374-L1-ABI-NLS-BGHpA pBW2283_pCAG-PV1-PYL-L1-FlpO-375C-NLS-BGHpA
Flp Recombinase Abscisic Acid pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpA pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA
VCre Recombinase Gibberellin pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpA pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpA
VCre Recombinase Gibberellin pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpA pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpA
VCre Recombinase Gibberellin pBW2550_pCAG-PV1-VCre-N277-L1-GID1-NLS-BGHpA pBW2570_pCAG-PV1-GAI-L1-VCre-278C-NLS-BGHpA
VCre Recombinase Rapalog pBW2582_pCAG-PV1-VCre-N172-L1-FRB-NLS-BGHpA pBW2586_pCAG-PV1-FKBP-L1-VCre-173C-NLS-BGHpA
VCre Recombinase Rapalog pBW2584_pCAG-PV1-VCre-N269-L1-FRB-NLS-BGHpA pBW2588_pCAG-PV1-FKBP-L1-VCre-270C-NLS-BGHpA
VCre Recombinase Rapalog pBW2618_pCAG-PV1-VCre-N277-L1-FRB-NLS-BGHpA pBW2620_pCAG-PV1-FKBP-L1-VCre-278C-NLS-BGHpA
VCre Recombinase Abscisic Acid pBW2583_pCAG-PV1-VCre-N172-L1-ABI-NLS-BGHpA pBW2587_pCAG-PV1-PYL-L1-VCre-173C-NLS-BGHpA
VCre Recombinase Abscisic Acid pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpA pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpA
VCre Recombinase Abscisic Acid pBW2619_pCAG-PV1-VCre-N277-L1-ABI-NLS-BGHpA pBW2621_pCAG-PV1-PYL-L1-VCre-278C-NLS-BGHpA
PhiC31 integrase Rapalog pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpA pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpA
PhiC31 integrase Rapalog pBW1345_pCAG-PV1-PhiC31-N396-L1-FRB-NLS-BGHpA pBW1358_pCAG-PV1-FKBP-L1-PhiC31-397C-NLS-BGHpA
PhiC31 integrase Rapalog pBW1346_pCAG-PV1-PhiC31-N428-L1-FRB-NLS-BGHpA pBW1359_pCAG-PV1-FKBP-L1-PhiC31-429C-NLS-BGHpA
PhiC31 integrase Rapalog pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpA pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpA
PhiC31 integrase Abscisic Acid pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpA pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA
PhiC31 integrase Abscisic Acid pBW1601_pCAG-PV1-PhiC31-N396-L1-ABI-NLS-BGHpA pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpA
PhiC31 integrase Abscisic Acid pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpA pBW1607_pCAG-PV1-PYL-L1-PhiC31-429C-BGHpA
PhiC31 integrase Abscisic Acid pBW1603_pCAG-PV1-PhiC31-N571-L1-ABI-NLS-BGHpA pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpA
PhiC31 integrase Gibberellin pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpA pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpA
TP901 integrase Rapalog pSD31_pCAG-PV1-TP901-N323-L1-FRB-NLS-BGHpA pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpA
TP901 integrase Rapalog pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpA pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpA
Bxb1 integrase Gibberellin pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpA pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpA
Cre Recombinase Blue Light pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpA pBW2690_pCAG-PV1-pMag-L1-iCre-230C-BGHpA
Cre Recombinase Blue Light pBW2693_pCAG-PV1-iCre-N251-L1-nMag-NLS-BGHpA pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpA
Cre Recombinase Blue Light pBW2697_pCAG-PV1-iCre-N256-L1-nMag-NLS-BGHpA pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpA
Cre Recombinase Blue Light pBW2701_pCAG-PV1-iCre-N270-L1-nMag-NLS-BGHpA pBW2702_pCAG-PV1-pMag-L1-iCre-271C-BGHpA
Flp Recombinase Blue Light pBW2665_pCAG-PV1-FlpO-N27-L1-nMag-NLS-BGHpA pBW2666_pCAG-PV1-pMag-L1-FlpO-28C-NLS-BGHpA
Flp Recombinase Blue Light pBW2669_pCAG-PV1-FlpO-N168-L1-nMag-NLS-BGHpA pBW2670_pCAG-PV1-pMag-L1-FlpO-169C-NLS-BGHpA
Flp Recombinase Blue Light pBW2673_pCAG-PV1-FlpO-N374-L1-nMag-NLS-BGHpA pBW2674_pCAG-PV1-pMag-L1-FlpO-375C-NLS-BGHpA
Flp Recombinase Blue Light pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpA pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpA
Flp Recombinase Gibberellin and
Abscisic Acid
pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpA
pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpA
pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Sub-Cloning Vectors For Creating Chemical or Light-inducible Proteins

A series of sub-cloning vectors containing CID or LID domains were assembled using standard molecular cloning techniques or Gibson isothermal assembly. For each CID or LID, two sub-cloning vectors were made such that a recombinase could be tagged with the domain on the C-terminus or N-terminus and a glycineserine rich linker. The cloning scheme is depicted in the image below. The backbone vectors are listed in the table underneath the figure and can be sorted by fusion protein or type of system.

System Fusion Plasmid
Rapalog C-terminal FKBP pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA
Rapalog N-terminal FRB pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA
Rapalog C-terminal FRB pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA
Rapalog N-terminal FKBP pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA
Abscisic Acid C-terminal PYL pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA
Abscisic Acid N-terminal ABI pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA
Abscisic Acid C-terminal ABI pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA
Abscisic Acid N-terminal PYL pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA
Gibberellin C-terminal GAI pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA
Gibberellin N-terminal GID1 pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA
Gibberellin C-terminal GID1 pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA
Gibberellin N-terminal GAI pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA
Magnet C-terminal nMag pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA
Magnet N-terminal pMag pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA
Magnet C-terminal pMag pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA
Magnet N-terminal nMag pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

The table below contains the full list of available plasmids from this article.

Plasmids from Article

ID Plasmid Purpose  
108723pBW2209_pCAG-PV1-iCre-N229-L1-GID1-NLS-BGHpAExpresses N-terminus to aa229 of Cre recombinase fused to GID1 CID
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108724pBW2229_pCAG-PV1-GAI-L1-iCre-230C-NLS-BGHpAExpresses aa230 to C-terminus of Cre recombinase fused to GAI CID
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108725pBW2210_pCAG-PV1-iCre-N251-L1-GID1-NLS-BGHpAExpresses N-terminus to aa251 of Cre recombinase fused to GID1 CID
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108726pBW2230_pCAG-PV1-GAI-L1-iCre-252C-NLS-BGHpAExpresses aa252 to C-terminus of Cre recombinase fused to GAI CID
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108727pBW2211_pCAG-PV1-iCre-N256-L1-GID1-NLS-BGHpAExpresses N-terminus to aa256 of Cre recombinase fused to GID1 CID
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108728pBW2231_pCAG-PV1-GAI-L1-iCre-257C-NLS-BGHpAExpresses aa257 to C-terminus of Cre recombinase fused to GAI CID
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108729pBW2426_pCAG-PV1-iCre-N270-L1-GID1-NLS-BGHpAExpresses N-terminus to aa270 of Cre recombinase fused to GID1 CID
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108730pBW2436_pCAG-PV1-GAI-L1-Cre-271C-NLS-BGHpAExpresses aa271 to C-terminus of Cre recombinase fused to GAI CID
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108731pBW2598_pCAG-PV1-iCre-N229-L1-FRB-NLS-BGHpAExpresses N-terminus to aa229 of Cre recombinase fused to FRB CID
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108732pBW2606_pCAG-PV1-FKBP-L1-iCre-230C-NLS-BGHpAExpresses aa230 to C-terminus of Cre recombinase fused to FKBP CID
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108733pBW2600_pCAG-PV1-iCre-N251-L1-FRB-NLS-BGHpAExpresses N-terminus to aa251 of Cre recombinase fused to FRB CID
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108734pBW2608_pCAG-PV1-FKBP-L1-iCre-252C-NLS-BGHpAExpresses aa252 to C-terminus of Cre recombinase fused to FKBP CID
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108735pBW2602_pCAG-PV1-iCre-N256-L1-FRB-NLS-BGHpAExpresses N-terminus to aa256 of Cre recombinase fused to FRB CID
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108736pBW2610_pCAG-PV1-FKBP-L1-iCre-257C-NLS-BGHpAExpresses aa257 to C-terminus of Cre recombinase fused to FKBP CID
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108737pBW2660_pCAG-PV1-iCre-N270-L1-FRB-NLS-BGHpAExpresses N-terminus to aa270 of Cre recombinase fused to FRB CID
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108738pBW2662_pCAG-PV1-FKBP-L1-iCre-271C-NLS-BGHpAExpresses aa271 to C-terminus of Cre recombinase fused to FKBP CID
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108739pBW2599_pCAG-PV1-iCre-N229-L1-ABI-NLS-BGHpAExpresses N-terminus to aa229 of Cre recombinase fused to ABI CID
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108740pBW2607_pCAG-PV1-PYL-L1-iCre-230C-NLS-BGHpAExpresses aa230 to C-terminus of Cre recombinase fused to PYL CID
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108741pBW2601_pCAG-PV1-iCre-N251-L1-ABI-NLS-BGHpAExpresses N-terminus to aa251 of Cre recombinase fused to ABI CID
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108742pBW2609_pCAG-PV1-PYL-L1-iCre-252C-NLS-BGHpAExpresses aa252 to C-terminus of Cre recombinase fused to PYL CID
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108743pBW2603_pCAG-PV1-iCre-N256-L1-ABI-NLS-BGHpAExpresses N-terminus to aa256 of Cre recombinase fused to ABI CID
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108744pBW2611_pCAG-PV1-PYL-L1-iCre-257C-NLS-BGHpAExpresses aa257 to C-terminus of Cre recombinase fused to PYL CID
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108745pBW2661_pCAG-PV1-iCre-N270-L1-ABI-NLS-BGHpAExpresses N-terminus to aa270 of Cre recombinase fused to ABI CID
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108746pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpAExpresses aa271 to C-terminus of Cre recombinase fused to PYL CID
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108747pBW2156_pCAG-PV1-FlpO-N27-L1-GID1-NLS-BGHpAExpresses N-terminus to aa27 of Flp recombinase fused to GID1 CID
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108748pBW2176_pCAG-PV1-GAI-L1-FlpO-28C-NLS-BGHpAExpresses aa28 to C-terminus of Flp recombinase fused to GAI CID
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108749pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpAExpresses N-terminus to aa168 of Flp recombinase fused to GID1 CID
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108750pBW2184_pCAG-PV1-GAI-L1-FlpO-169C-NLS-BGHpAExpresses aa169 to C-terminus of Flp recombinase fused to GAI CID
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108751pBW2173_pCAG-PV1-FlpO-N374-L1-GID1-NLS-BGHpAExpresses N-terminus to aa374 of Flp recombinase fused to GID1 CID
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108752pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpAExpresses aa375 to C-terminus of Flp recombinase fused to GAI CID
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108753pBW2175_pCAG-PV1-FlpO-N396-L1-GID1-NLS-BGHpAExpresses N-terminus to aa396 of Flp recombinase fused to GID1 CID
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108754pBW2195_pCAG-PV1-GAI-L1-FlpO-397C-NLS-BGHpAExpresses aa397 to C-terminus of Flp recombinase fused to GAI CID
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108755pBW2272_pCAG-PV1-FlpO-N27-L1-FRB-NLS-BGHpAExpresses N-terminus to aa27 of Flp recombinase fused to FRB CID
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108756pBW2273_pCAG-PV1-FKBP-L1-FlpO-28C-NLS-BGHpAExpresses aa28 to C-terminus of Flp recombinase fused to FKBP CID
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108757pBW2276_pCAG-PV1-FlpO-N168-L1-FRB-NLS-BGHpAExpresses N-terminus to aa168 of Flp recombinase fused to FRB CID
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108758pBW2277_pCAG-PV1-FKBP-L1-FlpO-169C-NLS-BGHpAExpresses aa169 to C-terminus of Flp recombinase fused to FKBP CID
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108759pBW2280_pCAG-PV1-FlpO-N374-L1-FRB-NLS-BGHpAExpresses N-terminus to aa374 of Flp recombinase fused to FRB CID
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108760pBW2281_pCAG-PV1-FKBP-L1-FlpO-375C-NLS-BGHpAExpresses aa375 to C-terminus of Flp recombinase fused to FKBP CID
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108761pBW2284_pCAG-PV1-FlpO-N396-L1-FRB-NLS-BGHpAExpresses N-terminus to aa396 of Flp recombinase fused to FRB CID
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108762pBW2285_pCAG-PV1-FKBP-L1-FlpO-397C-NLS-BGHpAExpresses aa397 to C-terminus of Flp recombinase fused to FKBP CID
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108763pBW2274_pCAG-PV1-FlpO-N27-L1-ABI-NLS-BGHpAExpresses N-terminus to aa27 of Flp recombinase fused to ABI CID
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108764pBW2275_pCAG-PV1-PYL-L1-FlpO-28C-NLS-BGHpAExpresses aa28 to C-terminus of Flp recombinase fused to PYL CID
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108765pBW2278_pCAG-PV1-FlpO-N168-L1-ABI-NLS-BGHpAExpresses N-terminus to aa168 of Flp recombinase fused to ABI CID
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108766pBW2279_pCAG-PV1-PYL-L1-FlpO-169C-NLS-BGHpAExpresses aa169 to C-terminus of Flp recombinase fused to PYL CID
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108767pBW2282_pCAG-PV1-FlpO-N374-L1-ABI-NLS-BGHpAExpresses N-terminus to aa374 of Flp recombinase fused to ABI CID
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108768pBW2283_pCAG-PV1-PYL-L1-FlpO-375C-NLS-BGHpAExpresses aa375 to C-terminus of Flp recombinase fused to PYL CID
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108769pBW2286_pCAG-PV1-FlpO-N396-L1-ABI-NLS-BGHpAExpresses N-terminus to aa396 of Flp recombinase fused to ABI CID
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108770pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpAExpresses aa397 to C-terminus of Flp recombinase fused to PYL CID
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108771pBW2542_pCAG-PV1-VCre-N172-L1-GID1-NLS-BGHpAExpresses N-terminus to aa172 of VCre recombinase fused to GID1 CID
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108772pBW2562_pCAG-PV1-GAI-L1-VCre-173C-NLS-BGHpAExpresses aa173 to C-terminus of VCre recombinase fused to GAI CID
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108773pBW2549_pCAG-PV1-VCre-N269-L1-GID1-NLS-BGHpAExpresses N-terminus to aa269 of VCre recombinase fused to GID1 CID
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108774pBW2569_pCAG-PV1-GAI-L1-VCre-270C-NLS-BGHpAExpresses aa270 to C-terminus of VCre recombinase fused to GAI CID
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108775pBW2550_pCAG-PV1-VCre-N277-L1-GID1-NLS-BGHpAExpresses N-terminus to aa277of VCre recombinase fused to GID1 CID
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108776pBW2570_pCAG-PV1-GAI-L1-VCre-278C-NLS-BGHpAExpresses aa278 to C-terminus of VCre recombinase fused to GAI CID
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108777pBW2582_pCAG-PV1-VCre-N172-L1-FRB-NLS-BGHpAExpresses N-terminus to aa172 of VCre recombinase fused to FRB CID
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108778pBW2586_pCAG-PV1-FKBP-L1-VCre-173C-NLS-BGHpAExpresses aa173 to C-terminus of VCre recombinase fused to FKBP CID
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108779pBW2584_pCAG-PV1-VCre-N269-L1-FRB-NLS-BGHpAExpresses N-terminus to aa269 of VCre recombinase fused to FRB CID
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108780pBW2588_pCAG-PV1-FKBP-L1-VCre-270C-NLS-BGHpAExpresses aa270 to C-terminus of VCre recombinase fused to FKBP CID
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108781pBW2618_pCAG-PV1-VCre-N277-L1-FRB-NLS-BGHpAExpresses N-terminus to aa277of VCre recombinase fused to FRB CID
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108782pBW2620_pCAG-PV1-FKBP-L1-VCre-278C-NLS-BGHpAExpresses aa278 to C-terminus of VCre recombinase fused to FKBP CID
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108783pBW2583_pCAG-PV1-VCre-N172-L1-ABI-NLS-BGHpAExpresses N-terminus to aa172 of VCre recombinase fused to ABI CID
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108784pBW2587_pCAG-PV1-PYL-L1-VCre-173C-NLS-BGHpAExpresses aa173 to C-terminus of VCre recombinase fused to PYL CID
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108785pBW2585_pCAG-PV1-VCre-N269-L1-ABI-NLS-BGHpAExpresses N-terminus to aa269 of VCre recombinase fused to ABI CID
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108786pBW2589_pCAG-PV1-PYL-L1-VCre-270C-NLS-BGHpAExpresses aa270 to C-terminus of VCre recombinase fused to PYL CID
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108787pBW2619_pCAG-PV1-VCre-N277-L1-ABI-NLS-BGHpAExpresses N-terminus to aa277of VCre recombinase fused to ABI CID
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108788pBW2621_pCAG-PV1-PYL-L1-VCre-278C-NLS-BGHpAExpresses aa278 to C-terminus of VCre recombinase fused to PYL CID
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108789pBW1340_pCAG-PV1-PhiC31-N233-L1-FRB-NLS-BGHpAExpresses N-terminus to aa233 of PhiC31 integrase fused to FRB CID
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108790pBW1353_pCAG-PV1-FKBP-L1-PhiC31-234C-NLS-BGHpAExpresses aa234 to C-terminus of PhiC31 integrase fused to FKBP CID
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108791pBW1345_pCAG-PV1-PhiC31-N396-L1-FRB-NLS-BGHpAExpresses N-terminus to aa396 of PhiC31 integrase fused to FRB CID
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108792pBW1358_pCAG-PV1-FKBP-L1-PhiC31-397C-NLS-BGHpAExpresses aa397 to C-terminus of PhiC31 integrase fused to FKBP CID
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108793pBW1346_pCAG-PV1-PhiC31-N428-L1-FRB-NLS-BGHpAExpresses N-terminus to aa428 of PhiC31 integrase fused to FRB CID
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108794pBW1359_pCAG-PV1-FKBP-L1-PhiC31-429C-NLS-BGHpAExpresses aa429 to C-terminus of PhiC31 integrase fused to FKBP CID
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108795pBW1349_pCAG-PV1-PhiC31-N571-L1-FRB-NLS-BGHpAExpresses N-terminus to aa571 of PhiC31 integrase fused to FRB CID
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108796pBW1362_pCAG-PV1-FKBP-L1-PhiC31-572C-NLS-BGHpAExpresses aa572 to C-terminus of PhiC31 integrase fused to FKBP CID
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108797pBW1599_pCAG-PV1-PhiC31-N233-L1-ABI-NLS-BGHpAExpresses N-terminus to aa233 of PhiC31 integrase fused to ABI CID
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108798pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpAExpresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID
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108799pBW1601_pCAG-PV1-PhiC31-N396-L1-ABI-NLS-BGHpAExpresses N-terminus to aa396 of PhiC31 integrase fused to ABI CID
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108800pBW1606_pCAG-PV1-PYL-L1-PhiC31-397C-BGHpAExpresses aa397 to C-terminus of PhiC31 integrase fused to PYL CID
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108801pBW1602_pCAG-PV1-PhiC31-N428-L1-ABI-NLS-BGHpAExpresses N-terminus to aa428 of PhiC31 integrase fused to ABI CID
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108802pBW1607_pCAG-PV1-PYL-L1-PhiC31-429C-BGHpAExpresses aa429 to C-terminus of PhiC31 integrase fused to PYL CID
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108803pBW1603_pCAG-PV1-PhiC31-N571-L1-ABI-NLS-BGHpAExpresses N-terminus to aa571 of PhiC31 integrase fused to ABI CID
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108804pBW1608_pCAG-PV1-PYL-L1-PhiC31-572C-BGHpAExpresses aa572 to C-terminus of PhiC31 integrase fused to PYL CID
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108805pBW2115_pCAG-PV1-PhiC31-N233-L1-GID1-NLS-BGHpAExpresses N-terminus to aa233 of PhiC31 integrase fused to GID1 CID
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108806pBW2116_pCAG-PV1-GAI-L1-PhiC31-234C-NLS-BGHpAExpresses aa234 to C-terminus of PhiC31 integrase fused to GAI CID
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108811pSD31_pCAG-PV1-TP901-N323-L1-FRB-NLS-BGHpAExpresses N-terminus to aa323 of TP901 integrase fused to GID1 CID
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108812pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpAExpresses aa324 to C-terminus of TP901 integrase with N-term FKBP and C-term NLS
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108813pSD35_pCAG-PV1-TP901-N326-L1-FRB-NLS-BGHpAExpresses N-terminus to aa326 of TP901 integrase fused to GID1 CID
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108814pSD36_pCAG-PV1-FKBP-L1-TP901-327C-BGHpAExpresses aa327 to C-terminus of TP901 integrase fused to FKBP
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108815pBW2121_pCAG-PV1-Bxb1-N468-L1-GID1-NLS-BGHpAExpresses N-terminus to aa468 of Bxb1 integrase fused to GID1 CID
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108816pBW2122_pCAG-PV1-GAI-L1-Bxb1-469C-NLS-BGHpAExpresses aa469 to C-terminus of Bxb1 integrase fused to GAI CID
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108817pBW2689_pCAG-PV1-iCre-N229-L1-nMag-NLS-BGHpAExpresses N-terminus to aa229 of Cre recombinase fused to nMag CID
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108818pBW2690_pCAG-PV1-pMag-L1-iCre-230C-BGHpAExpresses aa230 to C-terminus of Cre recombinase fused to pMag CID
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108819pBW2693_pCAG-PV1-iCre-N251-L1-nMag-NLS-BGHpAExpresses N-terminus to aa251 of Cre recombinase fused to nMag CID
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108820pBW2694_pCAG-PV1-pMag-L1-iCre-252C-BGHpAExpresses aa252 to C-terminus of Cre recombinase fused to pMag CID
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108821pBW2697_pCAG-PV1-iCre-N256-L1-nMag-NLS-BGHpAExpresses N-terminus to aa256 of Cre recombinase fused to nMag CID
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108822pBW2698_pCAG-PV1-pMag-L1-iCre-257C-BGHpAExpresses aa257 to C-terminus of Cre recombinase fused to pMag CID
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108823pBW2701_pCAG-PV1-iCre-N270-L1-nMag-NLS-BGHpAExpresses N-terminus to aa270 of Cre recombinase fused to nMag CID
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108824pBW2702_pCAG-PV1-pMag-L1-iCre-271C-BGHpAExpresses aa271 to C-terminus of Cre recombinase fused to pMag CID
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108825pBW2665_pCAG-PV1-FlpO-N27-L1-nMag-NLS-BGHpAExpresses N-terminus to aa27 of Flp recombinase fused to nMag CID
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108826pBW2666_pCAG-PV1-pMag-L1-FlpO-28C-NLS-BGHpAExpresses aa28 to C-terminus of Flp recombinase fused to pMag CID
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108827pBW2669_pCAG-PV1-FlpO-N168-L1-nMag-NLS-BGHpAExpresses N-terminus to aa168 of Flp recombinase fused to nMag CID
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108828pBW2670_pCAG-PV1-pMag-L1-FlpO-169C-NLS-BGHpAExpresses aa169 to C-terminus of Flp recombinase fused to pMag CID
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108829pBW2673_pCAG-PV1-FlpO-N374-L1-nMag-NLS-BGHpAExpresses N-terminus to aa374 of Flp recombinase fused to nMag CID
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108830pBW2674_pCAG-PV1-pMag-L1-FlpO-375C-NLS-BGHpAExpresses aa375 to C-terminus of Flp recombinase fused to pMag CID
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108831pBW2677_pCAG-PV1-FlpO-N396-L1-nMag-NLS-BGHpAExpresses N-terminus to aa396 of Flp recombinase fused to nMag CID
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108832pBW2678_pCAG-PV1-pMag-L1-FlpO-397C-NLS-BGHpAExpresses aa397 to C-terminus of Flp recombinase fused to pMag CID
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108833pBW2614_pCAG-PV1-GAI-L2-28-396-FlpO-ABI-NLS-BGHpAExpresses aa28 to aa396 of Flp recombinase fused to GAI and ABI CIDs
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108834pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpAInsert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminus
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108835pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminus
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108836pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpAInsert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminus
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108837pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminus
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108838pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpAInsert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminus
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108839pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminus
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108840pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpAInsert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminus
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108841pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminus
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108842pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpAInsert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminus
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108843pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus
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108845pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus
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108846pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpAInsert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminus
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108847pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminus
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108848pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpAInsert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminus
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108849pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpAInsert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus
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