PurposeExpresses N-terminus to aa374 of Flp recombinase fused to ABI CID
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||108767||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Cre/Lox, Synthetic Biology
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
Gene/Insert nameFlp recombinase fragment N-terminus to aa374
- Promoter pCAG
/ Fusion Protein
- ABI,NLS (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site BspEI (not destroyed)
Please visit https://www.biorxiv.org/content/10.1101/747121v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBW2282_pCAG-PV1-FlpO-N374-L1-ABI-NLS-BGHpA was a gift from Wilson Wong (Addgene plasmid # 108767 ; http://n2t.net/addgene:108767 ; RRID:Addgene_108767)
For your References section:High-performance chemical- and light-inducible recombinases in mammalian cells and mice. Weinberg BH, Cho JH, Agarwal Y, Pham NTH, Caraballo LD, Walkosz M, Ortega C, Trexler M, Tague N, Law B, Benman WKJ, Letendre J, Beal J , Wong WW. Nature Communications (2019) volume 10, Article number: 4845 10.1038/s41467-019-12800-7