We narrowed to 15,158 results for: pET

1. pET151-GFP-PP-RPN2 (925-953)

   Plasmid

   #73744

   Purpose

   Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

   Depositor

   Chris Hill

   Article

   VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

   Insert

   RPN2 (RPN2 Human)
   

   Tags

   6x His and GFP

   Expression

   Bacterial

   Mutation

   Construct possesses only residues 932-953 of RPN2

   Promoter

   T7

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

2. delNC C-His Ana GvpC in pET28a

   Plasmid

   #85736

   Purpose

   Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli

   Depositor

   Mikhail Shapiro

   Article

   Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

   Insert

   Gas vesicle protein C (truncated and his-tagged)
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Deletion of N and C terminal regions, addition of…

   Promoter

   T7

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b/GgVcl 884-1066 (aka Vt)

   Plasmid

   #46176

   Depositor

   Susan Craig

   Article

   Steimle et al J Biol Chem. 1999 Jun 25;274(26):18414-2

   Insert

   Vcl 884-1066 (VCL Chicken)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 884-1066 (tail domain)

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET51b-His-TEV-SNAP-tag_cx

   Plasmid

   #167273

   Purpose

   Overexpression of crystallizing SNAP-tag variant (P179R) in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAP-tag
   

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   P179R

   Promoter

   T7

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-ybbR-HIS-CBM-CohI

   Plasmid

   #58709

   Purpose

   Probing molecule to perform single molecule force spectroscopy experiments

   Depositor

   Hermann Gaub

   Article

   Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

   Insert

   CBM-CohI
   

   Tags

   HIS, HRV 3C, and ybbR

   Expression

   Bacterial

   Mutation

   changed Alanine 2 to Cysteine in CBM domain

   Promoter

   T7

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. HOXB13 (human) HIS-tag pET

   Plasmid

   #8543

   Depositor

   Corey Largman

   Article

   Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

   Insert

   HOXB13 (HOXB13 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by transcription/tra…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. HOXD12 (murine) HIS-tag pET

   Plasmid

   #8553

   Depositor

   Corey Largman

   Article

   Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

   Insert

   HOXD12 (Hoxd12 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   Fusion sequence is: ggatccATG

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

8. p139 pETF-GST RanBP10 L301I

   Plasmid

   #11092

   Depositor

   Ramesh Shivdasani

   Insert

   RanBP10 (Ranbp10 Mouse)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   L301I; see depositor comments below

   Available Since

   Dec. 16, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. HOXD4 (murine) HIS-tag pET

   Plasmid

   #8548

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXD4 (Hoxd4 Mouse)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by in vitro transcri…

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pET-28a-His6-SUMO-RABIF

    Plasmid

    #135556

    Purpose

    Express human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin

    Depositor

    Jingshi Shen

    Article

    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    RABIF (RABIF Human)
    

    Tags

    6xHis tag, thrombin recognition and cleavage site…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-sfGFP-GGS9-Cre-6xHis

    Plasmid

    #89343

    Purpose

    Expresses sfGFP fused to Cre in E.Coli

    Depositor

    David Liu

    Insert

    sf-GFP-GGS9-Cre-6xHis
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p230-pOsAct1::hpt-pZmUbi::BdPetC

    Plasmid

    #129649

    Purpose

    Rieske FeS overexpression

    Depositor

    Susanne von Caemmerer

    Article

    Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

    Insert

    CDS of PetC gene encoding for the Rieske FeS subunit
    

    Expression

    Plant

    Mutation

    Codon modified for Golden Gate

    Promoter

    pZmUbi

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. HOXC5 (human) HIS-tag pET

    Plasmid

    #8563

    Depositor

    Corey Largman

    Insert

    HOXC5 (HOXC5 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Starts at Amino Acid #2 (Ser); makes protein by W…

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

14. pET151-GFP-PP-RPN2 (918-932)

    Plasmid

    #73748

    Purpose

    Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

    Insert

    RPN2 (RPN2 Human)
    

    Tags

    6x His and GFP

    Expression

    Bacterial

    Mutation

    Construct possesses only residues 918-932 of RPN2

    Promoter

    T7

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

15. pET15b/GgVcl V1-258 (aka VD1)

    Plasmid

    #46173

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

    Insert

    Vcl 1-258 (VCL Chicken)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    aa 1-258 (VD1)

    Promoter

    T7

    Available Since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETCON-DnvLB16 M40R;W41H;H42S

    Plasmid

    #45125

    Depositor

    David Baker

    Article

    Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

    Insert

    DnvLB16 M40R;W41H;H42S
    

    Tags

    Aga2p for yeast surface display and c-myc epitope…

    Expression

    Yeast

    Mutation

    M40R;W41H;H42S to DnvLB16

    Promoter

    GAL

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. HOXB9 (murine) HIS-tag pET

    Plasmid

    #8541

    Depositor

    Corey Largman

    Article

    Chang et al Mol Cell Biol 1996 Apr;16(4):1734-45.

    Insert

    HOXB9 (Hoxb9 Mouse)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Missing the first 13 amino acids of HOXB9; makes …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pET28b-NmCas9-Flag D16A+H588A

    Plasmid

    #109033

    Purpose

    D16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

    Depositor

    Yan Zhang

    Article

    Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

    Insert

    CRISPR-associated endonuclease Cas9
    

    Tags

    Flag Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b/ECFP-GgVcl 884-1066 (Vt)

    Plasmid

    #46180

    Depositor

    Susan Craig

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Tags

    ECFP and His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available Since

    July 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET151-Vta1(280-330) S278C

    Plasmid

    #87739

    Purpose

    Bacterial expression of a VSL domain variant of S. cerevisiae Vta1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
    

    Tags

    6xHis-V5-TEV

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-277, mutated Ser 278 to Cys

    Promoter

    T7

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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