We narrowed to 5,308 results for: pcDNA3.1+

1. Cas9-FCPF

   Plasmid

   #220141

   Purpose

   Expresses Cas9-FCPF in mammalian cells

   Depositor

   Xinlai Cheng

   Article

   Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

   Insert

   Cas9-FCPF (cas9 S. Pyogenes, Synthetic)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

   Plasmid

   #201964

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   E162A,M164A,R167A

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

   Plasmid

   #201965

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   E198A, R199A,E200A, D201A

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

   Plasmid

   #201967

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   R232A, K233A, R234A, K235A, R236A

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

   Plasmid

   #201968

   Purpose

   Mammalian expression of mutated Translocon-associated protein subunit alpha

   Depositor

   Ville Paavilainen

   Article

   Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

   Insert

   SSR1 (SSR1 Human)
   

   Use

   Tags

   3xFLAG-tag

   Expression

   Mammalian

   Mutation

   W255A,N262E,K266E

   Promoter

   Available since

   Jan. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. XPO6-2Strep

   Plasmid

   #210356

   Purpose

   Expresses XPO6 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   XPO6 (XPO6 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRAM2-2Strep

   Plasmid

   #210374

   Purpose

   Expresses TRAM2 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   TRAM2 (TRAM2 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRAM1-2Strep

   Plasmid

   #210352

   Purpose

   Expresses TRAM1 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   TRAM1 (TRAM1 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SGTA-2Strep

   Plasmid

   #210353

   Purpose

   Expresses SGTA fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SGTA (SGTA Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHDR-PMG (pXK-1650)

    Plasmid

    #208858

    Purpose

    HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

    Depositor

    Kun Xu

    Article

    Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGP-CMV-jGCaMP8f L27A

    Plasmid

    #204140

    Purpose

    Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

    Depositor

    Katalin Torok

    Article

    Tran et al Front Cell Neurosci. 2023 May 18;17:1155

    Insert

    jGCaMP8f L27A
    

    Use

    Tags

    N-terminal His tag

    Expression

    Mammalian

    Mutation

    Leucine 27 changed to Alanine

    Promoter

    CMV

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ALIX(P801G)-mNeonGreen

    Plasmid

    #191192

    Purpose

    Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

    Depositor

    Jin Zhang

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX (P801G) (PDCD6IP Human, Synthetic)
    

    Use

    Tags

    6xHIS and mNeonGreen

    Expression

    Mammalian

    Mutation

    Pro 801 mutated to Gly

    Promoter

    CMV

    Available since

    Oct. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MCP-mCherry-TIS11B

    Plasmid

    #207670

    Purpose

    To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

    Depositor

    Christine Mayr

    Article

    Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

    Insert

    TIS11B (ZFP36L1 Human)
    

    Use

    Tags

    MCP-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFH2.7_Gag-dZF2-MCP-P2A-eGFP

    Plasmid

    #205523

    Purpose

    Express HIV Gag-delta-ZF2-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag delta ZF2-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ZF2 deleted

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFH2.14_GagZip-dp1dp6-MCP-P2A-eGFP

    Plasmid

    #205529

    Purpose

    Express HIV GagZip-dp1dp6-MCP-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    GagZip-dp1dp6-MCP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nucleocapsid replaced with leucine zipper, p1 and…

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFH2.17_GagZip-MCP-dpol-P2A-eGFP

    Plasmid

    #205532

    Purpose

    Express HIV GagZip-MCP-dpol-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP-dpol
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Nucleocapsid replaced with leucine zipper, pol fr…

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMyosin-miRFP718nano

    Plasmid

    #195748

    Purpose

    LC-Myosin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-LC-Myosin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pClathrin-miRFP718nano

    Plasmid

    #195747

    Purpose

    Clathrin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-clathrin light chain
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTubulin-miRFP718nano

    Plasmid

    #195746

    Purpose

    Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

    Insert

    miRFP718nano-human alpha-tubulin
    

    Use

    Tags

    miRFP718nano

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GFP-CBS (H65R)

    Plasmid

    #182924

    Purpose

    Mammalian expression plasmid for human CBS mutant H65R

    Depositor

    Philip Eaton

    Article

    Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

    Insert

    Cystathionine Beta-Synthase (CBS Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    changed histidine 65 to arginine

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge