165,916 results

1. Tet-O-FUW-KDM2B-P2A-mCherry

   Plasmid

   #203852

   Purpose

   Tet-inducible lentiviral plasmid expressing KDM2B-P2A-mCherry, with ORF specific barcode close to 3'LTR site

   Depositor

   Andrea Califano

   Article

   Obradovic et al Cancer Cell. 2023 May 8;41(5):933-949.e1

   Insert

   KDM2B (KDM2B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2957

   Plasmid

   #144433

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   HNF4A (HNF4A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TFORF2003

   Plasmid

   #142902

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXD3 (FOXD3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV R-CEPIA3mt

   Plasmid

   #140464

   Purpose

   Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

   Depositor

   Masamitsu Iino

   Article

   Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

   Insert

   R-CEPIA3mt
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1_AOX-FLAG

   Plasmid

   #177984

   Purpose

   All-in-one dox-inducible expression of Alternative Oxidase

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   AOX
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codon optimized cDNA for expression in human cells

   Promoter

   TRE

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 HA eIF4GI (1-1599)

   Plasmid

   #45640

   Depositor

   Nahum Sonenberg

   Article

   Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

   Insert

   eIF4GI transcript variant 2 (EIF4G1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-SAM

   Plasmid

   #102559

   Purpose

   Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Inserts

   MS2-P65-HSF1_T2A_Hygro
   dCAS9(D10A, N863A)-VP64_T2A_Blast
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9 and N55K in MS2

   Promoter

   CAG and EF1A

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pE-SUMO CRYAA

   Plasmid

   #80756

   Purpose

   Expression of human alpha A-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYAA (CRYAA Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDEST17-UnaG

   Plasmid

   #203485

   Purpose

   For recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.

   Depositor

   Yutaka Kodama

   Article

   Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

   Insert

   UnaG
   

   Use

   Tags

   Expression

   Bacterial and Plant

   Mutation

   Promoter

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a(+)_6xHIS-hHLA-A2 (MHC Class I heavy)-C-Tag

    Plasmid

    #187356

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    hHLA-A2 (MHC Class I heavy) with N-terminal 6xHIS tag, and C-terminal C-Tag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)_beta2M (MHC Class I light)

    Plasmid

    #187357

    Purpose

    Bacterial expression and refolding of gp100 pMHC

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Insert

    beta2M (MHC Class I light)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV5 TBRI-HA with various GS domain mutations

    Plasmid

    #19162

    Depositor

    Joan Massague

    Insert

    transforming growth factor, beta receptor 1 (TGFBR1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T204D (GS domain mutation)

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. JpExpress404 PTEN-LONG

    Plasmid

    #49417

    Purpose

    Bacterial Expression Vector

    Depositor

    Ramon Parsons

    Article

    Hopkins et al Science. 2013 Jul 26;341(6144):399-402.

    Insert

    PTEN-Long (PTEN )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T5

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PE-SUMO CRYBB1

    Plasmid

    #217675

    Purpose

    For expression of SUMO tagged human beta B1 crystallin

    Depositor

    Larry David

    Insert

    CRYBB1 (CRYBB1 Human)
    

    Use

    Tags

    6XHis and Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. C1-F-tractin-mCherry

    Plasmid

    #155218

    Purpose

    Monitor global F-actin distribution

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mCardinal

    Plasmid

    #51311

    Purpose

    Expresses far-red fluorescent protein mCardinal in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mCardinal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNTI935

    Plasmid

    #216638

    Purpose

    cpTurboID-PM (V5) Plasma membrane-localized cpTurboID, mammalian expression and lentivirus production

    Depositor

    Nicholas Ingolia

    Article

    Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

    Insert

    cpTurboID-PM
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. m168

    Plasmid

    #34886

    Depositor

    Irvin Chen

    Article

    Morizono et al Nat Med. 2005 Mar;11(3):346-52. Epub 200

    Insert

    Sindbis virus envelope protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

    Plasmid

    #110555

    Purpose

    pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

    Depositor

    Martin Pule

    Article

    Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

    Insert

    Anti-RD114 heavy chain
    

    Use

    Tags

    mIgG2A

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    EF-1 alpha core promoter

    Available Since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

    Plasmid

    #232880

    Purpose

    Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

    Depositor

    Paul DeCaen

    Article

    DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

    Insert

    ADP-ribosylation factor-like protein 13B (ARL13B Human)
    

    Use

    Lentiviral

    Tags

    GFP and RGECO1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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