168,238 results

1. hrGFP-Keap1

   Plasmid

   #28025

   Depositor

   Qing Zhong

   Article

   Fan et al Autophagy. 2010 Jul 22. 6(5):.

   Insert

   Keap1 (KEAP1 Human)
   

   Tags

   hrGFP

   Expression

   Mammalian

   Available Since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFlp-Tc

   Plasmid

   #222336

   Purpose

   Expresses Flp and tetracycline to enable excision of antibiotic resistance cassettes; flp-cl-sacB-pL1V-Lv1-TetAR-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB )
   flp (flp )
   cI (cI )
   

   Promoter

   cl, flp, and sacB

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDAC435

   Plasmid

   #195238

   Purpose

   Mammalian expression of Csm complex from separate promoters; RFP backbone (Used for RNA knockdown)

   Depositor

   Jennifer Doudna

   Article

   Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

   Insert

   FLAG-NLS-Csm1;FLAG-NLS-Csm2; FLAG-NLS-Csm3; FLAG-NLS-Csm4; FLAG-NLS-Csm5; FLAG-NLS-Cas6; crRNA; RFP
   

   Expression

   Mammalian

   Available Since

   Jan. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUltra-Plus

   Plasmid

   #161775

   Purpose

   3rd generation lentiviral multicistronic vector for mammalian expression of three genes of interest

   Depositor

   Jorge Luis Arias-Arias

   Type

   Empty backbone

   Use

   Lentiviral; Multicistronic

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX NPC1-FLAG-TiD

   Plasmid

   #199748

   Purpose

   Lentiviral construct to stably express NPC1 TurboID

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   NPC1 (NPC1 Human)
   

   Tags

   Flag and TurboID

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-N1-PARP1

   Plasmid

   #211578

   Purpose

   CMV driven PARP1-eGFP expressing construct with IRES-Neomycin selection

   Depositor

   Chris Lord

   Article

   Krastev et al Nat Cell Biol. 2022 Jan;24(1):62-73. doi

   Insert

   PARP1 (PARP1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-TO-hNGN2-BSD-mApple

   Plasmid

   #182308

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   hNGN2 (NEUROG2 Human)
   bsd-T2A-mycNLS-mApple
   

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. psgRNA

   Plasmid

   #114005

   Purpose

   express sgRNA. ColE1, Kan

   Depositor

   David Bikard

   Article

   Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

   Insert

   gRNA
   

   Promoter

   BBa_J23119

   Available Since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLv-HSA-uDys/eGFP

   Plasmid

   #26810

   Depositor

   Jeffrey Chamberlain

   Article

   Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

   Insert

   dystrophin (DMD Human)
   

   Use

   Lentiviral; Lentiviral plasmid backbone from l. n…

   Tags

   eGFP

   Mutation

   deleted R4-R23 deleted exons 71-78

   Available Since

   Sept. 12, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. VinculinTS

    Plasmid

    #26019

    Depositor

    Martin Schwartz

    Article

    Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

    Insert

    Vinculin tension sensor
    

    Expression

    Mammalian

    Available Since

    Sept. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYPQ173

    Plasmid

    #99907

    Purpose

    Express CRISPR-Act2.0 system which contains pco-dCas9-VP64 fusion protein and MS2-VP64 fusion protein linked by in-frame T2A (encoding Thosea asigna 'self-cleaving' 2A peptide) sequence

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Insert

    pco-dCas9-VP64-T2A-MS2-VP64
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-NTOM20-LOV2

    Plasmid

    #81009

    Purpose

    Expression of LOV2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    NTOM20

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV, p10

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLgw EcoDam-V5-RFC1

    Plasmid

    #59209

    Purpose

    Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Type

    Empty backbone

    Use

    Lentiviral; Damid

    Tags

    Dam and V5

    Expression

    Mammalian

    Promoter

    Heat Shock Minimal Promoter

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAG-GFP-IRES-CRE

    Plasmid

    #48201

    Purpose

    retroviral vector encoding for GFP and Cre-recombinase

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Inserts

    eGFP
    Cre
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CAG and linked by IRES

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBud-EGFP

    Plasmid

    #23027

    Depositor

    Andrea LeBlanc

    Article

    Jodoin et al PLoS One. 2009 . 4(8):e6647.

    Insert

    EGFP (eGFP )
    

    Expression

    Bacterial and Mammalian

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-Sequestosome1-N-18

    Plasmid

    #55132

    Purpose

    Localization: Membrane Scaffold, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Sequestosome1 (SQSTM1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pIB184-Km

    Plasmid

    #90195

    Purpose

    E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

    Depositor

    Indranil Biswas

    Article

    Hossain et al J Bacteriol. 2012 Jul;194(13):3486-94. d

    Type

    Empty backbone

    Tags

    None

    Expression

    Bacterial

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. LV anti-FITC-LaG16-NRR-T2A-BFP

    Plasmid

    #194929

    Purpose

    LV construct encoding NRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-NRR-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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