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His-GFP-Clathrin

Plasmid

#170858

Purpose

Mammalian expression of His-GFP-Clathrin.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Clathrin light polypeptide (Lca) (Clta Mouse)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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Laconic/pcDNA3.1(-)

Plasmid

#44238

Purpose

a genetically-encoded sensor for lactate concentration, transport and metabolic flux

Depositor

Luis Felipe Barros

Article

San Martin et al PLoS One. 2013;8(2):e57712. doi: 10.1371

Insert

Laconic (LACtate Optical Nano Indicator from CECs)

Use

Lactate biosensor

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-mTH-GFP

Plasmid

#99128

Purpose

An AAV genome that expresses GFP from the mouse TH promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

mTH

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pSL1119 (pDonor)

Plasmid

#160732

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA (Mini-Tn)

Use

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-miRFP670nano3

Plasmid

#184664

Purpose

Monomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmid

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

miRFP670nano3

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) CircRNA Mini Vector

Plasmid

#60648

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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MORF library without GFP and mCherry controls

Pooled Library

#192821

Purpose

Barcoded transcription factor ORF library for high throughput screening

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only
pC013 - Twinstrep-SUMO-huLwCas13a

Plasmid

#90097

Purpose

Twinstrep-SUMO-huLwCas13a for recombinant protein bacterial expression. Insert is human codon optimized but expresses well in bacteria.

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2017 Apr 13. pii: eaam9321. doi

Insert

LwCas13a

Use

CRISPR

Tags

6xHis-Twin Strep-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pBABE-hygro-BAP1-HA

Plasmid

#154020

Purpose

Mammalian Expression of HA-tagged Wild-Type BAP1

Depositor

Samuel Peña-Llopis

Article

Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

Insert

BAP1 (BAP1 Human)

Use

Retroviral

Tags

Hemagglutinin (HA)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF3265

Plasmid

#144741

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ARNTL2 (BMAL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHR-FUSN-mCh-Cry2olig

Plasmid

#101224

Purpose

FUS(1-214) fused to mCh-Cry2olig

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

FUS(aa1-214) (FUS Human)

Use

Lentiviral

Tags

mCherry-Cry2olig

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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FUW-hSNCA-GFP

Plasmid

#215489

Purpose

Lentiviral expression and easy visualization of alpha-synuclein in mammalian cells

Depositor

Ophir Shalem

Article

Santhosh Kumar et al Sci Adv. 2024 Feb 9;10(6):eadj4767. doi:

Insert

alpha-synuclein (SNCA Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Ubc

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Bxb1

Plasmid

#182142

Purpose

Expression of human codon optimized Bxb1

Depositor

David Liu

Article

Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

Insert

human codon optimized Bxb1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-deadMalibu-mScarlet

Plasmid

#229555

Purpose

Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

Depositor

Danielle Schmitt

Article

Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

Insert

Malibu-mScarlet

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSBtet-GP

Plasmid

#60495

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of GFP, rtTA and puromycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Transposon

Tags

Expression

Mammalian

Mutation

Promoter

TCE & RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pXPR_219

Plasmid

#164559

Purpose

Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

Depositor

Arlene Sharpe

Article

LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

Inserts

First sgRNA cassette
Second sgRNA cassette

Use

CRISPR and Lentiviral; Dual sgrna

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 and Mouse U6

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry- eMagBF - 5ptaseOCRL

Plasmid

#162254

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

mCherry- eMagBF - 5ptaseOCRL

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLV-EBFP2-nuc

Plasmid

#36085

Depositor

Pantelis Tsoulfas

Type

Empty backbone

Use

Lentiviral

Tags

NLS

Expression

Mammalian

Mutation

Replace EYFP of the vector pEYFP-Nuc from Clontec…

Promoter

Available since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pTBL3478 PB-PEmax-mTagBFP2

Plasmid

#226664

Purpose

PiggyBac cargo vector encoding PEmax and mTagBFP2

Depositor

Chang Liu

Article

Loveless et al bioRxiv 2021.11.05.467507

Inserts

PEmax
mTagBFP2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CAG and UBC

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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