169,450 results
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Plasmid#50378PurposeExpress mCherry and FLAG-tagged human CD59, a GPI-AP in mammalian cellsDepositorInsertmCherry
ExpressionMammalianPromoterSR alphaAvailable SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
DT40
Plasmid#80415PurposeExpresses 40 interrupted CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeatsDepositorInserthuman DMPK exons 11-15 with 40 interrupted CTG repeats (DMPK Human)
ExpressionMammalianAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
SBFP2-anti-AlfaTag nanobody
Plasmid#171826PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertSBFP2-anti-AlfaTag nanobody
Tags6xHis and SEP tag (6xD)ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (AAV1)
Viral Prep#98928-AAV1PurposeReady-to-use AAV1 particles produced from pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 (#98928). In addition to the viral particles, you will also receive purified pAAV.CAG.Flex.Ruby2sm-Flag.WPRE.SV40 plasmid DNA. CAG-driven, Cre-dependent expression of Ruby2sm-Flag spaghetti monster construct. Spaghetti monster constructs are not fluorescent ("dark") to allow full range of antibody staining. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby2sm-Flag (spaghetti monster construct)Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 WT
Plasmid#78260PurposepsiCHECK2 dual luciferase reporter harboring a fully complementary let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase geneDepositorInsertfully complimentary let-7 target site
UseLuciferase; Microrna activityExpressionMammalianAvailable SinceJune 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
AYBEv3
Plasmid#193967PurposeAn engineered adenine transversion base editor (AYBEv3, A-to-C and/or A-to-T base editor) driven by EF1a promoter, guide RNAs compatible with spCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertecTadA8e-nSpCas9(D10A)-MPG(v3)
ExpressionMammalianMutationG163R, N169S, S198A, K202A, G203A, S206A, K210APromoterhU6, EF1a, CBhAvailable SinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
AdEasier-1 cells (strain)
Bacterial Strain#16399DepositorBacterial ResistanceAmpicillin and StreptomycinSpeciesEscherichia coliAvailable SinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCRIS-PITChv2-BSD-dTAG (BRD4)
Plasmid#91792PurposePITCh dTAG donor vector for BSD-P2A-2xHA-FKBP_F36V knock-in into the N-terminus of the human BRD4 locus.DepositorInsertBSD-P2A-2xHA-FKBP_F36V
UseCRISPRExpressionMammalianMutationPhenylalanine 36 to valine in FKBP12PromoterPromoterlessAvailable SinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
TFORF2249
Plasmid#143184PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 fosA
Plasmid#112889PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertfosA
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAct-dCBEevoCDA1
Plasmid#195515PurposeUbiquitous expression of dCBEevoCDA1 in DrosophilaDepositorInsertdCBE-evoCDA1
UseCRISPRExpressionInsectPromoteract5CAvailable SinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
DsRed2-pBAD
Plasmid#54608PurposeLocalization: Protein Expression Vector , Excitation: 558, Emission: 583DepositorTypeEmpty backboneTags6xHis and DsRed2ExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti UBC hNLRP3-mNG
Plasmid#218642PurposeExpresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selectionDepositorAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-mito-LAR-GECO1.2
Plasmid#61245PurposeExpresses LAR-GECO1.2 in the mitochondria in mammalian cellsDepositorInsertLAR-GECO1.2
Tagsa duplex of the mitochondrial targeting signal of…ExpressionMammalianMutationSubstitutions relative to R-GECO1.2: N45I/A47R/E1…PromoterCMVAvailable SinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-ymScarletI3-URA3
Plasmid#224075PurposeYeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selectionDepositorInsertymScarletI3
TagsymScarletI3ExpressionYeastAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (AAV9)
Viral Prep#104491-AAV9PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7s-WPRE (#104491). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7s-WPRE plasmid DNA. Synapsin-driven, Cre-dependent, GCaMP7s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-3xmNG::LEU2
Plasmid#227594PurposeIntegration vector for expressing Lifeact-3xmNeonGreenDepositorInsertLifeact-3xmNeonGreen::LEU2
Tags3xmNeonGreenExpressionYeastMutationTruncated ABP140 (aa 1-17 only)PromoterAbp140Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pScarlet-N1
Plasmid#128060PurposeMammalian expression vector for C terminal mScarlet-I fusionsDepositorTypeEmpty backboneTagsmScarlet-IExpressionMammalianAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
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mito-gTEMP_pcDNA3
Plasmid#109117PurposeMitochondria targetting gTEMPDepositorInsertmito-gTEMP
ExpressionMammalianPromoterCMVAvailable SinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only