We narrowed to 6,241 results for: nls

1. pH-PABE-7-sgRNA

   Plasmid

   #115621

   Purpose

   Targeted A to G in rice

   Depositor

   Caixia Gao

   Article

   Li et al Genome Biol. 2018 May 29;19(1):59. doi:

   Insert

   wtTadA-TadA7.10-nCas9-3*NLS
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMpGWB215

   Plasmid

   #68606

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Tags

   Nuclear localization signal-tagged Citrine (Citri…

   Expression

   Plant

   Available Since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSL2620 (pTniQ_Vch)

   Plasmid

   #200892

   Purpose

   Expresses human codon-optimized V. cholerae TniQ from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchTniQ
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. vas-Cas9.874Z

   Plasmid

   #112687

   Purpose

   Express hSpCas9 under vasa promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   vasa

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3xFLAG-dCas9/pMXs-IG

   Plasmid

   #51258

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGLTR-X-PURO

   Plasmid

   #58246

   Purpose

   GATEWAY-compatible lentiviral conditional RNAi delivery vector; SFFV-driven expression of TetR and Puromycin selection

   Depositor

   Stephan Geley

   Article

   Sigl et al PLoS One. 2014 May 19;9(5):e97764. doi:

   Insert

   Puro(R)
   

   Use

   Lentiviral and RNAi; Gateway destionation vector

   Tags

   TetR-NLS-T2A

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBK-YE2-BE3

   Plasmid

   #85176

   Purpose

   Expresses YE2-BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

   Insert

   YEE-BE3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMB22

   Plasmid

   #122244

   Purpose

   Lentivirus delivery for stable expression of AsCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Promoter

   EFs

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

   Plasmid

   #200910

   Purpose

   3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

   Depositor

   Debojyoti Chakraborty

   Insert

   3XHA_den1FnCas9_KRAB_eGFP
   

   Use

   CRISPR

   Tags

   3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Changed Glutamic Acid to Arginine at 1369 position

   Promoter

   CBH

   Available Since

   Sept. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSL2621 (pCas8_Vch)

    Plasmid

    #200893

    Purpose

    Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas8
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSL2623 (pCas6_Vch)

    Plasmid

    #200895

    Purpose

    Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas6
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLV-REPORT(PGK)-12xHRE

    Plasmid

    #172334

    Purpose

    hypoxic responsive reporter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3xFLAG-dCas9/pMSCVneo

    Plasmid

    #134982

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 3xFLAG-dCas9/pMSCVpuro

    Plasmid

    #134983

    Purpose

    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR and Retroviral

    Tags

    3xFLAG tag and NLS

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    LTR

    Available Since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

    Plasmid

    #158793

    Purpose

    Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

    Insert

    Neuraminidase A/NL/109/03
    

    Tags

    TwinStrep_sfGFP_tetrabrachion

    Expression

    Mammalian

    Mutation

    AA61-469

    Promoter

    CMV

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSL2622 (pCas7_Vch)

    Plasmid

    #200894

    Purpose

    Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCas7
    

    Tags

    NLS

    Expression

    Mammalian

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGem-nHyPer2:sHyper2

    Plasmid

    #84737

    Purpose

    Simultaneous expresion of nuclear and stromal HyPer2 in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Insert

    HyPer2 targeted to nucleus
    

    Tags

    NLS-SV40

    Expression

    Plant

    Promoter

    pFMV/p35S

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-T3-eSpCAS9-NG-pA

    Plasmid

    #131466

    Purpose

    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized eSpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1-GoldenGate

    Plasmid

    #47388

    Purpose

    Expression plasmid for TALE proteins compatible with GoldenGate cloning system

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Mar;10(3):239-42. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBK-EE-BE3

    Plasmid

    #85175

    Purpose

    Expresses EE-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    EE-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge