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Plasmid#110287PurposeA backbone vector for cell surface display in E. coli by Ligation Independent Cloning (LIC)DepositorInsertyfaL
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
IDG_CLIC3_OE_1
Plasmid#161657PurposeOverexpresses CLIC3DepositorInsertCLIC3 (CLIC3 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_FliC_IRES_mCherry_PGK_tBFP
Plasmid#85438PurposeLentiviral vector for Gal4 inducible S. Typhi Flagellin with mCherry reporter and constitutive tBFP expressionDepositorInsertS. typhi FliC
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDG_CLIC5_OE_1
Plasmid#161641PurposeOverexpresses CLIC5DepositorInsertCLIC5 (CLIC5 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLICER
Plasmid#197288PurposeConjugative plasmid that facilitates seamless deletions in D. radiodurans following insertion of a seamless deletion cassette. Contains an origin of transferDepositorInsertI-SceI Endonuclease
UseSynthetic Biology; Bacterial and yeast cloningTagsExpressionMutationPromoterAvailable sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES_iSeroSnFR_cytosolic
Plasmid#214132PurposeFluorescent biosensor for intracellular serotonin (5-HT)DepositorInsertiSeroSnFR_cytosolic
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
ptdTomato.LIC.HXGPRT
Plasmid#83365PurposePlasmid contains LIC cassette upstream of an in-frame copy of tdTomato and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInserttdTomato
UseToxoplasma gondiiTagstdTomatoExpressionMutationPromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLIC
Plasmid#27989DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
CLIC6_pCSdest
Plasmid#53789DepositorInsertCLIC6
UseTagsExpressionMammalianMutationPromoterSP6 and CMVAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYFP.LIC.DHFR
Plasmid#83114PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and DHFR-TS selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
IDG_CLIC2_OE_1
Plasmid#161642PurposeOverexpresses CLIC2DepositorInsertCLIC2 (CLIC2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYFP.LIC.HXGPRT
Plasmid#83128PurposePlasmid contains LIC cassette upstream of an in-frame copy of YFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus.DepositorInsertYFP
UseToxoplasma gondiiTagsYFPExpressionMutationPromoterEndogenous GeneAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry.LIC.HXGPRT
Plasmid#83364PurposePlasmid contains LIC cassette upstream of an in-frame copy of mCherry and HXGPRT selectable marker cassette for endogenous tagging on the gene locusDepositorInsertmCherry
UseToxoplasma gondiiTagsmCherryExpressionMutationPromoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_B_miR-7
Plasmid#220468PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version B)DepositorInsertSUMO_Eu1
UseTags3xHAExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_B_miR-7.M4
Plasmid#220469PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version B)DepositorInsertSUMO_Eu1
UseTags3xHAExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7
Plasmid#220464PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSliceRep_A_miR-7.M4
Plasmid#220465PurposeDual luciferase reporter for RNAi, with target site within a tunable ORF (version A)DepositorInsertSuperfolder GFP
UseTags3xFLAGExpressionMammalianMutationPromoterMinimal CMVAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z-Y-L
Plasmid#153546Purposethis is signaling incompetent version of LIC-Z where all Tyrosine residues in ITAMs are mutated to leucineDepositorInserthuman TCR ζ-Chain (CD247 Human)
UseTagsExpressionMammalianMutationwith 6 Y in 3 ITAMs mutated to LPromoterCMVAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
LIC TAL Effector Assembly Kit
Plasmid Kit#1000000023PurposeLIC (ligation-independent cloning) TALE repeat unit library of 2-mer fragments for assembly in an expression ready TALEN construct in 2 hierarchical assembly steps. Semiâautomated TALE generationDepositorAvailable sinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
DiLiCre 2.0
Plasmid#198752PurposeA Cre recombinase that is expressed upon doxycycline when combined with the UbC-blasticidine-P2A-TETon-3G transactivator. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertCre-PhoCI-ERT2-ERT2
UseLentiviralTagsCre, kept in a photocleavable site, followed by 2…ExpressionBacterial and MammalianMutationPromoterTRE3GVAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only