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We narrowed to 4,700 results for: golden gate

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Showing: 3981 - 4000 of 4700 results

  8. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid
    #227645
    Purpose
    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.
    Depositor
    Richard Murray
    Article
    Kapasiawala et al ACS Synth Biol. 2024 Dec 20;13(12):3976-
    Insert
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    Use
    Synthetic Biology
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    227645-plasmid-map-sequence-id-452394

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  9. pJB55

    Plasmid
    #226310
    Purpose
    E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.
    Depositor
    David Savage
    Article
    Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d
    Insert
    CsoS2 with V(T/S)G to AAA CsoS2 MR1-5
    Expression
    Bacterial
    Mutation
    VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…
    Promoter
    pTRC
    Available Since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226310-plasmid-map-sequence-id-449922

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  10. TL1 alphaDdCBE 3_NC (right, DddA11-C)

    Plasmid
    #222005
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC right)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222005-plasmid-map-sequence-id-438647

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  11. TL1 alphaL_DdCBE 1 (left, DddA11-C)

    Plasmid
    #221996
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alphaL-1 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221996-plasmid-map-sequence-id-438699

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  12. TL1 alphaL_DdCBE 4 (right, DddA11-N)

    Plasmid
    #221999
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE repeats (alphaL-4 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    DddA: S1330I, A1341V, N1342S, E1370K, T1380I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221999-plasmid-map-sequence-id-438705

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  13. TL1 alphaDdCBE 1-2 (right, DddA11-N)

    Plasmid
    #222000
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-1/2 right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | TALE: S1330I, A1341V,…
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222000-plasmid-map-sequence-id-438640

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  14. TL1 alphaDdCBE 2 (left, DddA11-C)

    Plasmid
    #222001
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-2 left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222001-plasmid-map-sequence-id-438748

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  15. TL1 alphaDdCBE 3_CN (left, DddA11-C)

    Plasmid
    #222002
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN left)-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222002-plasmid-map-sequence-id-438715

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  16. TL1 alphaDdCBE 3_CN (right, DddA11-N)

    Plasmid
    #222003
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_CN right)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222003-plasmid-map-sequence-id-438752

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  17. TL1 alphaDdCBE 3_NC (left, DddA11-N)

    Plasmid
    #222004
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Stephen Ekker
    Article
    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE repeats (alpha-3_NC left)-TALE CTD-DddA11 1397N-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222004-plasmid-map-sequence-id-443120

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  18. pTE5423_p3WJdB-glcO60

    Plasmid
    #221194
    Purpose
    DNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor system
    Depositor
    Tobias Erb
    Article
    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0
    Insert
    T7 promoter-glcO60-3WJdB
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter with downstream glcO60 operator
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221194-plasmid-map-sequence-id-441878

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  19. pro::EX1-2(intΔNACs&ΔMYBs):GFP

    Plasmid
    #218572
    Purpose
    Transcriptional reporter for ARF7 promoter
    Depositor
    Anthony Bishopp
    Insert
    AT5G20730 (NPH4 Mustard Weed)
    Expression
    Plant
    Mutation
    Mutation genomic sequence 85nt, 86nt from TA to C…
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    218572-plasmid-map-sequence-id-437877

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Showing: 3981 - 4000 of 4700 results