We narrowed to 15,158 results for: pET

1. pETM11-SUMO3-KSR1-CA3-sfGFP (C-KSR)

   Plasmid

   #217766

   Purpose

   Fluorescent reporter for ceramide (for bacterial expression and purification)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Protein purification (6xhis tag, tev site, sumo3 …

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   CA3 domain aa 317-400 plus vector-based linker LE…

   Promoter

   T7 promoter

   Available Since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

   Plasmid

   #116955

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. BPV00554 pET21GG2-His(6)-BECN(248-450)

   Plasmid

   #136685

   Purpose

   Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag

   Depositor

   Colin Garvie

   Article

   Ranaghan et al Biochemistry. 2017 Dec 26;56(51):6639-66

   Insert

   Beclin-1 ECD/BARA (BECN1 Human)
   

   Tags

   Hexa-Histadine

   Expression

   Bacterial

   Mutation

   DNA optimized for E. coli expression

   Promoter

   T7

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-(pos36)GFP-9xGGS-Cre-6xHis

   Plasmid

   #62938

   Purpose

   Expression of (+36)GFP-GGS9-Cre-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (ps36)GFP-9xGGS-Cre
   

   Tags

   (pos36)GFP and 6xHis tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-DUET-GST-eIF4G1-His6, eIF4E

   Plasmid

   #37232

   Depositor

   Jon Lorsch

   Article

   Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

   Inserts

   TIF4631 (TIF4631 Budding Yeast)
   CDC33 (CDC33 Budding Yeast)
   

   Tags

   GST and His6

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pET mRuby2 LIC cloning vector (H6-mRuby2)

   Plasmid

   #86934

   Purpose

   pET LIC cloning vector for Biotin-His6-tev-yORF-mRuby2

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Tags

   6xHis, AviTag (biotin), TEV cleavage site, and mR…

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET mRuby2 LIC cloning vector (u-mRuby2)

   Plasmid

   #86932

   Purpose

   pET LIC cloning vector for yORF-mRuby2

   Depositor

   Chris Jeans

   Type

   Empty backbone

   Tags

   mRuby2

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-45b-HOXD13(+10A)-IDR-mCherry

   Plasmid

   #145282

   Purpose

   Bacterial Expression of 6xHis HOXD13 (+10A) IDR mCherry Fusion Protein

   Depositor

   Denes Hnisz

   Article

   Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

   Insert

   HOXD13(+10A)-IDR (HOXD13 Human)
   

   Expression

   Bacterial

   Mutation

   A-repeat expanded

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. FabF (KASII) pet28 N-terminal His Tag

   Plasmid

   #75017

   Purpose

   Expresses the ketosynthase FabF/KASII from the E. coli fatty acid synthase

   Depositor

   Michael Burkart

   Article

   Worthington et al ACS Chem Biol. 2006 Dec 20;1(11):687-91.

   Insert

   FabF (fabF E. coli)
   

   Tags

   His Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 21, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pET-(neg27)GFP-GGS9-Cre-6xHis

    Plasmid

    #89253

    Purpose

    Expresses GFP with -27 theoretical charge fused to Cre in E.Coli

    Depositor

    David Liu

    Insert

    (neg27)GFP-GGS9-Cre-6xHis
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28a-GFP-Patronin CC 868–1457

    Plasmid

    #59053

    Purpose

    for E. coli expression of Patronin CC truncation

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    Patronin (Patronin Fly)
    

    Tags

    His, Strep, TEV, and sfGFP

    Expression

    Bacterial

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

12. Affibody EGFR:1907.BirA*-pET301/NT-DEST

    Plasmid

    #174691

    Purpose

    Plasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*

    Depositor

    Jean Gariepy

    Article

    Alwash et al ACS Pharmacol Transl Sci. 2022 Feb 1;5(2

    Insert

    Anti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
    

    Tags

    HIS tag

    Expression

    Bacterial

    Mutation

    R118G mutation in BirA

    Promoter

    T7 promoter

    Available Since

    Jan. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a-CTLA4-FLN-ELP-His-ybbr

    Plasmid

    #168038

    Purpose

    Expresses CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

    Depositor

    Michael Nash

    Article

    Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

    Insert

    Cytotoxic T-lymphocyte associated protein 4 (CTLA4 Human)
    

    Tags

    3x ELP linker, 6x Histag, ddFLN4 (C18S), and ybbr…

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET mClover LIC cloning vector (H6-mClover)

    Plasmid

    #86931

    Purpose

    pET LIC cloning vector for Biotin-His6-tev-yORF-mClover

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    6xHis, AviTag (biotin), TEV cleavage site, and mC…

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

    Plasmid

    #167849

    Purpose

    Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

    Depositor

    Cheryl Arrowsmith

    Article

    Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

    Insert

    nsp10, nsp16
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pET mClover LIC cloning vector (u-mClover)

    Plasmid

    #86929

    Purpose

    pET LIC cloning vector for yORF-mClover

    Depositor

    Chris Jeans

    Type

    Empty backbone

    Tags

    mClover

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28b – E. coli RecE residues 564-868

    Plasmid

    #104534

    Purpose

    Expresses E. coli RecE residues 564-868

    Depositor

    Charles Bell

    Article

    Zhang et al Structure. 2009 May 13;17(5):690-702. do

    Insert

    RecE residues 564-866 (C-terminal nuclease domain)
    

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. HOXD8 (human,partial) HIS-tag pET

    Plasmid

    #8569

    Depositor

    Corey Largman

    Insert

    HOXD8 (HOXD8 Human)
    

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Partial protein containing 4 AA N-terminal to HD …

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-32M human DKK1c (183–266)

    Plasmid

    #132626

    Purpose

    expresses human DKK1c (183–266) in E. coli

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    DKK1 (183–266)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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