Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,166 results for: golden gate

Clear keywords
Showing: 4061 - 4080 of 7166 results

  4. L4857 dsRedExpress2 reporter, VEGFR2 NTEVp, VEGFR1 CTEVp in PiggyBac Transposon Vector

    Plasmid
    #244187
    Purpose
    PiggyBac transposon vector for expression of dsRed-Express2 synTF promoter; constitutive expression of VEGFR2 NTEVp chain, VEGFR1 CTEVp chain, and mNeonGreen-P2A-PuroR selection marker
    Depositor
    Joshua Leonard
    Publication
    Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158
    Insert
    dsRed-Express2 under synTF responsive promoter; VEGFR2 NTEVp chain with WT NTEVp; VEGFR1 CTEVp chain; mNeonGreen-P2A-PuroR
    Use
    Synthetic Biology
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    CTEVp_190K
    Promoter
    hEF1a
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244187-plasmid-map-sequence-id-489486

    Enlarge

  14. LbPlant toolbox II

    Plasmid kit
    #1000000263
    Purpose
    Plasmids and parts for hierarchical assembly for expression in Lactiplantibacillus plantarum. Recommended to be used in combination with LbPlant Toolbox I.
    Depositor
    Georg Fritz
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression
    Cloning type
    Golden Gate (MoClo)
    Available since
    July 22, 2025
    Availability
    Academic Institutions and Nonprofits only

  15. pET26b-BsaI

    Plasmid
    #233523
    Purpose
    Purification vector with BsaI cut sites for streamlined cloning
    Depositor
    Carl Denard
    Type
    Empty backbone
    Tags
    6xHis-tag and pelB leader
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233523-plasmid-map-sequence-id-474147

    Enlarge

  16. pSEVAb887

    Plasmid
    #217504
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb88 backbone, pUC ori
    Depositor
    Nico Claassens
    Publication
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217504-plasmid-map-sequence-id-444288

    Enlarge

  17. Tad-CBE(CCT2)

    Plasmid
    #235071
    Purpose
    Expresses Tad-CBE(CCT2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(CCT2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235071-plasmid-map-sequence-id-466783

    Enlarge

  18. Tad-CBE(ACT4)

    Plasmid
    #235060
    Purpose
    Expresses Tad-CBE(ACT4) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACT4)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235060-plasmid-map-sequence-id-466786

    Enlarge

  19. Tad-CBE(ACC2)

    Plasmid
    #235061
    Purpose
    Expresses Tad-CBE(ACC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235061-plasmid-map-sequence-id-466785

    Enlarge

  20. Tad-CBE(TCT1)

    Plasmid
    #235065
    Purpose
    Expresses Tad-CBE(TCT1) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCT1)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235065-plasmid-map-sequence-id-466798

    Enlarge

Showing: 4061 - 4080 of 7166 results