We narrowed to 5,047 results for: IRES

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pLV-H1-shScramble-hsynapsin-EGFP

Plasmid

#220795

Purpose

control shRNA plasmid with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-Junb

Plasmid

#178441

Purpose

doxycycline-inducible expression of Mouse Junb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Junb (Junb Mouse)

Use

Lentiviral

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-Cep123-C2 domain

Plasmid

#73329

Purpose

Encodes the distal appendage targeting domain of isoform 1 of Cep123 (residues 500-600) fused to EGFP

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 4, 2016

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Irf1

Plasmid

#139833

Purpose

doxycycline-inducible expression of mouse Irf1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Irf1 (Irf1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-H1-shSRGAP2B/C#2-hsynapsin-EGFP

Plasmid

#220797

Purpose

shRNA plasmid against human SRGAP2B/C genes (#2) with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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p420-3

Plasmid

#71257

Purpose

luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

Depositor

Peter Cockerill

Article

Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

Insert

3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

GM-CSF minimal

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Stat3

Plasmid

#139805

Purpose

doxycycline-inducible expression of mouse Stat3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Stat3 (Stat3 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

Plasmid

#174899

Purpose

Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

Plasmid

#190153

Purpose

Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only AA #1-94

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-H1-shSRGAP2A-hsynapsin-EGFP

Plasmid

#220798

Purpose

shRNA plasmid against human SRGAP2A gene with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pENTR1A hu_ncOGT WT

Plasmid

#154281

Purpose

Entry vector encoding non-tagged O-GlcNAc Transferase

Depositor

Suzanne Walker

Article

Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

Insert

O-GlcNAc Transferase (OGT Human)

Use

Gateway entry vector

Tags

2x FLAG and mKate2

Mutation

WT OGT

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPGS dnTcf-4(deltaN41)

Plasmid

#19284

Depositor

Eric Fearon

Article

Kolligs et al Mol Cell Biol. 1999 Aug . 19(8):5696-706

Insert

Tcf-4 (TCF4 Human)

Use

Retroviral

Tags

Flag

Mutation

Dominant negative, lacks N-terminal 41 amino acids

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Irf2

Plasmid

#139814

Purpose

doxycycline-inducible expression of mouse Irf2 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Irf2 (Irf2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.GFAP.iGluSnFr.WPRE.SV40

Plasmid

#98930

Purpose

AAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Has Service

AAV1, AAV5, and AAV9

Insert

iGluSnFr

Use

AAV

Expression

Mammalian

Promoter

GFAP

Available Since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-TFEC

Plasmid

#178447

Purpose

doxycycline-inducible expression of Human TFEC in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

TFEC (TFEC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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FpG5.BFP.Reporter

Plasmid

#134967

Purpose

Lentiviral vector for enhancer reporter assay

Depositor

Beat Bornhauser

Article

Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

mini Fgf4 promoter

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Cep123 (CCDC123/Cep89)

Plasmid

#67787

Purpose

This plasmid encodes EGFP fused to the N-terminus of isoform 1 of Cep123 (CCDC123/Cep89)

Depositor

Michel Bornens

Article

Sillibourne et al Biol Open. 2013 Apr 9;2(6):535-45. doi:

Insert

Cep123 (CEP89 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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6K-loop-->R GLUT1-GFP

Plasmid

#200098

Purpose

human GLUT1 with K-->R mutation of 6 lysines in the major cytoplasmic loop and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 225, 229, 230, …

Promoter

CMV

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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11K-cyto-->R GLUT1-GFP

Plasmid

#200096

Purpose

human GLUT1 with K-->R mutations for all 11 cytosolic lysines and a C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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