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Jonathan Kagan Lab: pLxIS-containing domains are biochemically flexible regulators of interferons and metabolism

Unpublished

Plasmids from Article

ID Plasmid Purpose
221239pMSCV-FKBP-emptyExpression of FLAG-4xFKBP scaffold in mammalian cells by retroviral transduction
221240pMSCV-FKBP-STING(pLxIS)Expression of STING CTT in mammalian cells by retroviral transduction
221241pMSCV-FKBP-STING(pLxIS) S365AExpression of mutant STING CTT in mammalian cells by retroviral transduction
221242pMSCV-FKBP-STING(pLxIS) L373AExpression of mutant STING CTT in mammalian cells by retroviral transduction
221243pMSCV-FKBP-STING(FL)Expression of STING without transmembrane domain in mammalian cells by retroviral transduction
221244pMSCV-FKBP-TRIF(Nterm)Expression of TRIF N-terminus (1-222) in mammalian cells by retroviral transduction
221245pMSCV-FKBP-TRIF(pLxIS) S209AExpression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction
221246pMSCV-FKBP-TRIF(pLxIS) L193AExpression of mutant TRIF pLxIS domain (1-222) in mammalian cells by retroviral transduction
221247pMSCV-FKBP-TRIF(trunc 1-23)Expression of TRIF truncation 1 (24-222) in mammalian cells by retroviral transduction
221248pMSCV-FKBP-TRIF(trunc 1-43)Expression of TRIF truncation 2 (44-222) in mammalian cells by retroviral transduction
221249pMSCV-FKBP-TRIF(trunc 1-63)Expression of TRIF truncation 3 (64-222) in mammalian cells by retroviral transduction
221250pMSCV-FKBP-TRIF(trunc 1-83)Expression of TRIF truncation 4 (84-222) in mammalian cells by retroviral transduction
221251pMSCV-FKBP-TRIF(trunc 1-103)Expression of TRIF truncation 5 (104-222) in mammalian cells by retroviral transduction
221252pMSCV-FKBP-TRIF(trunc 1-123)Expression of TRIF truncation 6 (124-222) in mammalian cells by retroviral transduction
221253pMSCV-FKBP-TRIF(trunc 1-143)Expression of TRIF truncation 7 (144-222) in mammalian cells by retroviral transduction
221254pMSCV-FKBP-TRIF(trunc 1-163)Expression of TRIF truncation 8 (164-222) in mammalian cells by retroviral transduction
221255pMSCV-FKBP-TRIF(trunc 1-183)Expression of TRIF truncation 9 (184-222) in mammalian cells by retroviral transduction
221256pMSCV-FKBP-MAVS(pLxIS)Expression of MAVS pLxIS (393-441) in mammalian cells by retroviral transduction
221258pMSCV-FKBP-MAVS(pLxIS) E433AExpression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transduction
221259pMSCV-FKBP-MAVS(trunc Cterm)Expression of MAVS truncation (393-431) in mammalian cells by retroviral transduction
221260pMSCV-FKBP-IRF3Expression of IRF3 in mammalian cells by retroviral transduction
221261pMSCV-FKBP-TASL(pLxIS)Expression of TASL pLxIS (266-298) in mammalian cells by retroviral transduction
221262pMSCV-FKBP-TASL(Cterm)Expression of TASL Cterm (102-298) in mammalian cells by retroviral transduction
221263pMSCV-FKBP-TASL(FL)Expression of TASL in mammalian cells by retroviral transduction
221264pMSCV-FKBP-TASL(pLxIS-PLPLR)Expression of TASL pLxIS-STING PLPLR in mammalian cells by retroviral transduction
221265pMSCV-TASLExpression of TASL in mammalian cells by retroviral transduction
221266pMSCV-TASL-PLPLRExpression of TASL-STING PLPLR in mammalian cells by retroviral transduction
221267pMSCV-TASL-NEMOExpression of TASL-NEMO IKK binding site in mammalian cells by retroviral transduction
221268pMSCV-FKBP-IRSp53(pLxIS)Expression of IRSp53 pLxIS (247-375) in mammalian cells by retroviral transduction
221269pMSCV-FKBP-IRSp53(pLxIS) S269AExpression of IRSp53 pLxIS mutant in mammalian cells by retroviral transduction
221271pMSCV-FKBP-GMIP(pLxIS) S31AExpression of GMIP pLxIS mutant in mammalian cells by retroviral transduction
221272pMSCV-FKBP-IRSp53(trunc N1)Expression of IRSp53 N-terminal truncation 1 (257-375) in mammalian cells by retroviral transduction
221273pMSCV-FKBP-IRSp53(trunc C1)Expression of IRSp53 C-terminal truncation 1 (247-355) in mammalian cells by retroviral transduction
221274pMSCV-FKBP-IRSp53(trunc C2)Expression of IRSp53 C-terminal truncation 2 (247-335) in mammalian cells by retroviral transduction
221276pMSCV-FKBP-IRSp53(trunc C4)Expression of IRSp53 C-terminal truncation 4 (247-295) in mammalian cells by retroviral transduction
221277pMSCV-FKBP-IRSp53(trunc C5)Expression of IRSp53 C-terminal truncation 5 (247-275) in mammalian cells by retroviral transduction
221278pMSCV-FKBP-GMIP(trunc N1)Expression of GMIP N-terminal truncation 1 (16-84) in mammalian cells by retroviral transduction
221279pMSCV-FKBP-GMIP(trunc C1)Expression of GMIP C-terminal truncation 1 (1-64) in mammalian cells by retroviral transduction
221280pMSCV-FKBP-GMIP(trunc C2)Expression of GMIP C-terminal truncation 2 (1-44) in mammalian cells by retroviral transduction
221281pMSCV-FKBP-IRSp53 with STING pLxISExpression of IRSp53 with STING pLxIS (NLV265RLL) in mammalian cells by retroviral transduction
221282pMSCV-FKBP-STING with IRSp53 pLxISExpression of STING with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transduction
221283pMSCV-FKBP-STING L373A with IRSp53 pLxISExpression of STING L373A with IRSp53 pLxIS (RLL361NLV) in mammalian cells by retroviral transduction
221284pMSCV-FKBP-RFP(pLxIS-PLPLR)Expression of RFP (pLxIS-PLPLR) in mammalian cells by retroviral transduction
221285pMSCV-FKBP-Myoglobin(pLxIS-PLPLR)Expression of Myoglobin (pLxIS-PLPLR) in mammalian cells by retroviral transduction
221286pMSCV-FKBP-p27(pLxIS-PLPLR)Expression of p27 (pLxIS-PLPLR) in mammalian cells by retroviral transduction
221287pMSCV-FKBP-H3(pLxIS-PLPLR)Expression of H3 (pLxIS-PLPLR) in mammalian cells by retroviral transduction
221288pMSCV-FKBP-CNOT6Expression of CNOT6 (pLxIS) in mammalian cells by retroviral transduction
221289pMSCV-FKBP-DAGLAExpression of DAGLA (pLxIS) in mammalian cells by retroviral transduction
221290pMSCV-FKBP-DZIP1LExpression of DZIP1L (pLxIS) in mammalian cells by retroviral transduction
221291pMSCV-FKBP-GARNL3Expression of GARNL3 (pLxIS) in mammalian cells by retroviral transduction
221292pMSCV-FKBP-JCADExpression of JCAD (pLxIS) in mammalian cells by retroviral transduction
221293pMSCV-FKBP-KIF5CExpression of KIF5C (pLxIS) in mammalian cells by retroviral transduction
221295pMSCV-FKBP-MAP2K4Expression of MAP2K4 (pLxIS) in mammalian cells by retroviral transduction
221296pMSCV-FKBP-NMD3Expression of NMD3 (pLxIS) in mammalian cells by retroviral transduction
221297pMSCV-FKBP-NSD3Expression of NSD3 (pLxIS) in mammalian cells by retroviral transduction
221298pMSCV-FKBP-TMPRSS3Expression of TMPRSS3 (pLxIS) in mammalian cells by retroviral transduction
221299pMSCV-FKBP-ZBTB44Expression of ZBTB44 (pLxIS) in mammalian cells by retroviral transduction
221300pMSCV-FKBP-ZC3H12BExpression of ZC3H12B (pLxIS) in mammalian cells by retroviral transduction
221301pMSCV-FKBP-ARHGAP45Expression of ARHGAP45 (pLxIS) in mammalian cells by retroviral transduction
221303pMSCV-FKBP-ASAP2Expression of ASAP2 (pLxIS) in mammalian cells by retroviral transduction
221304pMSCV-FKBP-ASAP3(Cterm)Expression of ASAP3 (Cterm pLxIS) in mammalian cells by retroviral transduction
221307pMSCV-FKBP-FESExpression of FES (pLxIS) in mammalian cells by retroviral transduction
221309pMSCV-FKBP-NOSTRINExpression of NOSTRIN (pLxIS) in mammalian cells by retroviral transduction
221310pMSCV-FKBP-OPHN1Expression of OPHN1 (pLxIS) in mammalian cells by retroviral transduction
221311pMSCV-FKBP-SYNDAPIN2Expression of SYNDAPIN2 (pLxIS) in mammalian cells by retroviral transduction
221312pMSCV-FKBP-TUBAExpression of TUBA (pLxIS) in mammalian cells by retroviral transduction
221313pMSCV-MAVSExpression of MAVS in mammalian cells by retroviral transduction
221314pMSCV-MAVS(pLxIS trunc)Expression of MAVS truncated pLxIS domain in mammalian cells by retroviral transduction
221315pMSCV-MAVS(IRSp53 pLxIS)Expression of MAVS chimera with IRSp53 pLxIS domain in mammalian cells by retroviral transduction
221316pMSCV-MAVS(GMIP pLxIS)Expression of MAVS chimera with GMIP pLxIS domain in mammalian cells by retroviral transduction
221317pRetroX-TRE3G-IRSp53Dox-inducible expression of IRSp53 in mammalian cells by retroviral transduction
221318pRetroX-TRE3G-GMIPDox-inducible expression of GMIP in mammalian cells by retroviral transduction
221319pRetroX-TRE3G-IRSp53 S269DDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction
221320pRetroX-TRE3G-IRSp53 P278D P281DDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction
221321pRetroX-TRE3G-IRSp53 P428LDox-inducible expression of IRSp53 mutant in mammalian cells by retroviral transduction
221322pRetroX-TRE3G-GMIP S31DDox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction
221323pRetroX-TRE3G-GMIP P363D P364D P366DDox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction
221324pRetroX-TRE3G-GMIP P564DDox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction
221325pRetroX-TRE3G-GagDox-inducible expression of Gag in mammalian cells by retroviral transduction
221326pRetroX-TRE3G-TirDox-inducible expression of Tir in mammalian cells by retroviral transduction
221327pRetroX-TRE3G-SopEDox-inducible expression of SopE in mammalian cells by retroviral transduction
221328pRetroX-TRE3G-YopEDox-inducible expression of YopE in mammalian cells by retroviral transduction
221329pRetroX-TRE3G-IRSp53-T2A-Cdc42(G12V)Dox-inducible expression of FLAG-IRSp53-T2A-HA-Cdc42 G12V in mammalian cells by retroviral transduction
221330pRetroX-TRE3G-IRSp53-T2A-Rac1(G12V)Dox-inducible expression of FLAG-IRSp53-T2A-HA-Rac1 G12V in mammalian cells by retroviral transduction
221331pFastBac-GST-TBK1TBK1 protein overexpression in Sf9 insect cells
221332pET28a-FKBP-emptyProtein overexpression in bacteria
221333pET28a-FKBP-STING(pLxIS)Overexpression of STING CTT in bacteria
221334pET28a-FKBP-TRIF(Nterm)Overexpression of TRIF Nterm domain in bacteria
221335pET28a-FKBP-MAVS(pLxIS)Overexpression of MAVS pLxIS domain in bacteria
221336pET28a-FKBP-IRSp53(pLxIS)Overexpression of IRSp53 pLxIS domain in bacteria
221337pET28a-FKBP-GMIP(pLxIS)Overexpression of GMIP pLxIS domain in bacteria

Antibodies from Article