pRetroX-TRE3G-GMIP P363D P364D P366D
(Plasmid
#221323)
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PurposeDox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221323 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRetroX-TRE3G
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Vector typeRetroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGMIP
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SpeciesM. musculus (mouse)
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MutationP363D P364D P366D
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Entrez GeneGmip (a.k.a. 5031419I10Rik)
- Promoter TRE3G
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains a 24bp deletion in the PGK promoter. This deletion is not known to affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRetroX-TRE3G-GMIP P363D P364D P366D was a gift from Jonathan Kagan (Addgene plasmid # 221323 ; http://n2t.net/addgene:221323 ; RRID:Addgene_221323)