pFastBac-GST-TBK1
(Plasmid
#221331)
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PurposeTBK1 protein overexpression in Sf9 insect cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 221331 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFastBac
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTBK1
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SpeciesM. musculus (mouse)
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Entrez GeneTbk1 (a.k.a. 1200008B05Rik)
- Promoter polyhedrin
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Tag
/ Fusion Protein
- 6xHis-TEV-2xGST-TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac-GST-TBK1 was a gift from Jonathan Kagan (Addgene plasmid # 221331 ; http://n2t.net/addgene:221331 ; RRID:Addgene_221331)