Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 5,193 results for: golden gate

Clear keywords
Showing: 4161 - 4180 of 5193 results

  7. pAAV-ITR-CMV-mCherry-1xU6-LeuIGI2

    Plasmid
    #217368
    Purpose
    Expresses E. coli leucine tRNA variant "LeuIGI2" and a wild-type mCherry reporter; can be packaged into AAV
    Depositor
    Abhishek Chatterjee
    Article
    Huang et al ACS Synth Biol. 2024 Jul 19;13(7):2141-2
    Insert
    E. coli leucine tRNA mutant, "LeuIGI2" for TAG suppression
    Use
    AAV
    Expression
    Mammalian
    Mutation
    C2G, C3G, G6U, A7G, U77C, C78G, G81C, G82C, U83C
    Promoter
    U6
    Available Since
    July 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    217368-plasmid-map-sequence-id-430295

    Enlarge

  9. pKS1334

    Plasmid
    #239508
    Purpose
    Express CDT2 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeur
    Depositor
    John Dueber
    Insert
    NcCDT2-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    239508-plasmid-map-sequence-id-477192

    Enlarge

  10. pLII-CRISPR for CKA4

    Plasmid
    #238521
    Purpose
    Plant expression of Cas9 and gRNAs against A. thaliana CK2alpha4
    Depositor
    Michael Hothorn
    Insert
    AtCK2alpha4 (AT2G23070 Mustard Weed)
    Use
    CRISPR
    Expression
    Plant
    Available Since
    June 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    238521-plasmid-map-sequence-id-477173

    Enlarge

  11. pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP

    Plasmid
    #239450
    Purpose
    Codon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker.
    Depositor
    Alexis Komor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239450-plasmid-map-sequence-id-476833

    Enlarge

  12. pSG1016-2xBsaI-SaKKH_P2A_EGFP

    Plasmid
    #239461
    Purpose
    Codon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.
    Depositor
    Alexis Komor
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    239461-plasmid-map-sequence-id-476832

    Enlarge

  15. pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))

    Plasmid
    #239448
    Purpose
    Orthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.
    Depositor
    Alexis Komor
    Inserts
    S.p. gRNA backbone
    S.a. gRNA backbone
    PuroR-T2A-BFP(C>T_screening)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    BFP: T65S, S72S, V145F, K206K, H231L
    Promoter
    EF1alpha, U6, and mU6
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239448-plasmid-map-sequence-id-476590

    Enlarge

  18. pJJB112

    Plasmid
    #239493
    Purpose
    Express peroxisomal Venus
    Depositor
    John Dueber
    Insert
    Ubi-R-Venus-ePTS1
    Expression
    Yeast
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239493-plasmid-map-sequence-id-476401

    Enlarge

  19. pVL1172

    Plasmid
    #239535
    Purpose
    Express CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    CpPT1(52-408)-mTurquoise2
    Expression
    Yeast
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239535-plasmid-map-sequence-id-476352

    Enlarge

Showing: 4161 - 4180 of 5193 results