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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,282 results for: golden gate

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Showing: 4161 - 4180 of 5282 results

  1. pVL1173

    Plasmid
    #239536
    Purpose
    Express CpPT1 membrane protein cargo with N-terminal mTurqoise2 marker but without any targeting sequence and Venus ER marker
    Depositor
    John Dueber
    Insert
    mTurquoise2-CpPT1(52-408)
    Expression
    Yeast
    Available since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239536-plasmid-map-sequence-id-479033

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  2. pKS1616

    Plasmid
    #239547
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available since
    July 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239547-plasmid-map-sequence-id-479031

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  3. pN4

    Plasmid
    #233521
    Purpose
    Nb Vector Plasmid (under galactose induction), AmpR (E. coli) and TRP1 (yeast), Cen6/ARS
    Depositor
    Carl Denard
    Type
    Empty backbone
    Expression
    Bacterial and Yeast
    Promoter
    pGAL1-10
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    233521-plasmid-map-sequence-id-471179

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  4. pSEVAb847

    Plasmid
    #217502
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb84 backbone, pRO1600/ColE1 ori
    Depositor
    Nico Claassens
    Publication
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217502-plasmid-map-sequence-id-444279

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  5. pSEVAb867

    Plasmid
    #217503
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb86 backbone, p15A ori
    Depositor
    Nico Claassens
    Publication
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217503-plasmid-map-sequence-id-444286

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  6. pSEVAb897

    Plasmid
    #217505
    Purpose
    pSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP ori
    Depositor
    Nico Claassens
    Publication
    van den Brink et al ACS Synth Biol. 2025 Jun 5. doi: 10.1021
    Insert
    superfolder green fluorescent protein (sfGFP)
    Use
    Synthetic Biology
    Mutation
    S30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…
    Available since
    July 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217505-plasmid-map-sequence-id-444281

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  9. pMKR07

    Plasmid
    #240097
    Purpose
    A CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native host
    Depositor
    Adrian Keatinge-Clay
    Publication
    Wang et al Res Sq [Preprint]. 2025 Mar 27:rs.3.rs-6
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    ermE* promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240097-plasmid-map-sequence-id-478442

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  10. pKS1429

    Plasmid
    #239514
    Purpose
    Express Yea4p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative control
    Depositor
    John Dueber
    Insert
    ScYea4p-mTurquoise2-2xCrk_SH3
    Expression
    Yeast
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239514-plasmid-map-sequence-id-478375

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  11. pPSG779

    Plasmid
    #239532
    Purpose
    CRISPR plasmid withCas9 and sgRNA targeting YBR197C locus in S. cerevisiae
    Depositor
    John Dueber
    Insert
    sgRNA_YBR197C
    Use
    CRISPR
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239532-plasmid-map-sequence-id-478373

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  12. pKS1173

    Plasmid
    #239507
    Purpose
    Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptides
    Depositor
    John Dueber
    Insert
    sfCherry3C(1-10)-dPTS1
    Expression
    Yeast
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239507-plasmid-map-sequence-id-478369

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  13. pKS1620

    Plasmid
    #239551
    Purpose
    Express GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrCPR-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239551-plasmid-map-sequence-id-478368

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  14. pKS1617

    Plasmid
    #239548
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239548-plasmid-map-sequence-id-478367

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  15. pKS1618

    Plasmid
    #239549
    Purpose
    Express GAL-inducible G8H cytochrome P450 tagged with mTagBFP2 and inactive Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-mTagBFP2-2xCrk_SH3
    Expression
    Yeast
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239549-plasmid-map-sequence-id-478366

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  16. pKS1615

    Plasmid
    #239546
    Purpose
    Express GAL-inducible G8H cytochrome P450 and associated reductase as membrane protein cargoes and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPR
    Depositor
    John Dueber
    Insert
    CrG8H-2xCrk_SH3
    Expression
    Yeast
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    239546-plasmid-map-sequence-id-478363

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Showing: 4161 - 4180 of 5282 results