Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2.
Mucha M, Lai Z, McKenzie NJ, Matra F, Boudes M, Flanigan SF, Alejo-Vinogradova MT, Monger C, Zhang Q, Nimmo D, Healy E, Silva AJ, Angelov D, Reck DM, Holland G, Atmaca ZE, King HE, Hamilton M, Glancy E, Nolan J, Weatheritt RJ, Bell O, Vermeulen M, Davidovich C, Bracken AP
EMBO J. 2025 Dec;44(23):6979-7020. doi: 10.1038/s44318-025-00616-9. Epub 2025 Oct 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 247004 | pFB1.HMBP-Prs.AEBP2iso2 | Expresses human AEBP2iso2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247005 | pFB1.HMBP-Prs.AEBP2iso1 | Expresses human AEBP2iso1 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247006 | pFB1.HMBP-Prs.AEBP2iso2.mt1A | Expresses human AEBP2iso2.mt1A (with twelve glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247007 | pFB1.HMBP-Prs.AEBP2iso2.mt1K | Expresses human AEBP2iso2.mt1K (with twelve glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247008 | pFB1.HMBP-Prs.AEBP2iso2.mt1∆ | Expresses human AEBP2iso2.mt1∆ in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247009 | pFB1.HMBP-Prs.AEBP2iso2.mt2A | Expresses human AEBP2iso2.mt2A (with fifteen asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247010 | pFB1.HMBP-Prs.AEBP2iso2.mt2K | Expresses human AEBP2iso2.mt2K (with twelve asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247011 | pFB1.HMBP-Prs.AEBP2iso2.mt2∆ | Expresses human AEBP2iso2.mt2∆ in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247012 | pFB1.HMBP-Prs.AEBP2iso2.mt1A2A | Expresses human AEBP2iso2.mt1A2A (with 27 asp/glu-to-ala mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247013 | pFB1.HMBP-Prs.AEBP2iso2.mt1K2K | Expresses human AEBP2iso2.mt1K2K (with 27 asp/glu-to-lys mutations) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247014 | pFB1.HMBP-Prs.AEBP2iso2.mt1∆2∆ | Expresses human AEBP2iso2.mt1∆2∆ in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247015 | pBIG1A.HMBP.PrS.PRC2-trunc | Expression of EZH2, SUZ12 (70-685), EED (80-411), RBBP4 under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag. |
| 247016 | pMB.AEBP2LTEV | Expression of AEBP2LTEV (AEBP2 long isoform that can be converted to an AEBP2 short isoform by TEV cleavage) in insect cells |
| 247017 | pMB.PRC2-AEBP2LTEV-JARID2 | Expression of MBP-EZH2, MBP-SUZ12, MBP-EED, MBP-RBBP4, AEBP2LTEV (AEBP2 long isoform that can be converted to an AEBP2 short isoform by TEV cleavage), MBP-JARID2 in insect cells, MBP tags are C3-cleav |
| 247018 | pMB.MBP.JARID2 | Expression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable (PreScission Protease) |
| 247019 | pWidom601.CDKN2Bp.601 | Two Widom601 sequences, with a short linker with sequence from the CDKN2B locus between them. For large scale PCR amplification of DNA for nucleosome refolding. |