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pBIG1A.HMBP.PrS.PRC2-trunc
(Plasmid #247015)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247015 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pBIG1a
  • Vector type
    Insect Expression
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    EZH2
  • Species
    H. sapiens (human)
  • Entrez Gene
    EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    SUZ12
  • Species
    H. sapiens (human)
  • Mutation
    aa 70-685 only
  • Entrez Gene
    SUZ12 (a.k.a. CHET9, IMMAS, JJAZ1)

Gene/Insert 3

  • Gene/Insert name
    EED
  • Species
    H. sapiens (human)
  • Mutation
    aa 80-411 only
  • Entrez Gene
    EED (a.k.a. COGIS, HEED, WAIT1)

Gene/Insert 4

  • Gene/Insert name
    RBBP4
  • Species
    H. sapiens (human)
  • Entrez Gene
    RBBP4 (a.k.a. NURF55, RBAP48, lin-53)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBIG1A.HMBP.PrS.PRC2-trunc was a gift from Chen Davidovich (Addgene plasmid # 247015 ; http://n2t.net/addgene:247015 ; RRID:Addgene_247015)

  • For your References section:

    Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2. Mucha M, Lai Z, McKenzie NJ, Matra F, Boudes M, Flanigan SF, Alejo-Vinogradova MT, Monger C, Zhang Q, Nimmo D, Healy E, Silva AJ, Angelov D, Reck DM, Holland G, Atmaca ZE, King HE, Hamilton M, Glancy E, Nolan J, Weatheritt RJ, Bell O, Vermeulen M, Davidovich C, Bracken AP. EMBO J. 2025 Dec;44(23):6979-7020. doi: 10.1038/s44318-025-00616-9. Epub 2025 Oct 30. 10.1038/s44318-025-00616-9 PubMed 41168462
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