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pMB.PRC2-AEBP2LTEV-JARID2
(Plasmid #247017)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 247017 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pMB.Pos5
  • Vector type
    Insect Expression
  • Selectable markers
    Spectinomycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Gentamicin, 100 & 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    EZH2
  • Species
    H. sapiens (human)
  • Entrez Gene
    EZH2 (a.k.a. ENX-1, ENX1, EZH2b, KMT6, KMT6A, WVS, WVS2)
  • Tag / Fusion Protein
    • MBP (N terminal on backbone)

Gene/Insert 2

  • Gene/Insert name
    SUZ12
  • Species
    H. sapiens (human)
  • Entrez Gene
    SUZ12 (a.k.a. CHET9, IMMAS, JJAZ1)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Gene/Insert 3

  • Gene/Insert name
    EED
  • Species
    H. sapiens (human)
  • Entrez Gene
    EED (a.k.a. COGIS, HEED, WAIT1)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Gene/Insert 4

  • Gene/Insert name
    RBBP4
  • Species
    H. sapiens (human)
  • Entrez Gene
    RBBP4 (a.k.a. NURF55, RBAP48, lin-53)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Gene/Insert 5

  • Gene/Insert name
    AEBP2LTEV
  • Species
    H. sapiens (human)
  • Mutation
    AEBP2 long isoform that can be converted to an AEBP2 short isoform by TEV cleavage
  • Entrez Gene
    AEBP2
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Gene/Insert 6

  • Gene/Insert name
    JARID2
  • Species
    H. sapiens (human)
  • Entrez Gene
    JARID2 (a.k.a. DIDDF, JMJ)
  • Tag / Fusion Protein
    • MBP (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMB.PRC2-AEBP2LTEV-JARID2 was a gift from Chen Davidovich (Addgene plasmid # 247017 ; http://n2t.net/addgene:247017 ; RRID:Addgene_247017)

  • For your References section:

    Auto-inhibition of PRC2 by the broadly expressed long isoform of AEBP2. Mucha M, Lai Z, McKenzie NJ, Matra F, Boudes M, Flanigan SF, Alejo-Vinogradova MT, Monger C, Zhang Q, Nimmo D, Healy E, Silva AJ, Angelov D, Reck DM, Holland G, Atmaca ZE, King HE, Hamilton M, Glancy E, Nolan J, Weatheritt RJ, Bell O, Vermeulen M, Davidovich C, Bracken AP. EMBO J. 2025 Dec;44(23):6979-7020. doi: 10.1038/s44318-025-00616-9. Epub 2025 Oct 30. 10.1038/s44318-025-00616-9 PubMed 41168462
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