We narrowed to 5,193 results for: golden gate
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Plasmid#226913PurposeMinimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientationDepositorTypeEmpty backboneExpressionPlantAvailable SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only
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XanthoMoClo Xanthobacter Genetic Toolkit
Plasmid Kit#1000000259PurposePlasmids and parts for modular assembly of single and multi-gene plasmids for any Xanthobacter or Roseixanthobacter strain.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial ExpressionCloning TypeGolden Gate (MoClo)Available SinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF5x1-mRuby2-miRE.FF5-bGH
Plasmid#235259PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 3DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2-miRE.FF5-bGH
Plasmid#235258PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 3DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1619
Plasmid#239550PurposeExpress GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertCrCPR-mTagBFP2-2xCrk_SH3
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP
Plasmid#239588Purposeexpresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRaDepositorInsertsgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)
UseSleeping beauty (sb) transposonExpressionMammalianPromoterhU6Available SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1140
Plasmid#239501PurposeExpress GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptidesDepositorInsertsfCherry3C(1-10)-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1170
Plasmid#239504PurposeExpress GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptideDepositorInsertsfCherry3C(1-10)-dPTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1577
Plasmid#239525PurposeExpress Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative controlDepositorInsertScVba2p-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1141
Plasmid#239502PurposeExpress GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 5 high affinity SH3 peptidesDepositorInsertsfCherry3C(1-10)-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1601
Plasmid#239526PurposeExpress CpPT1(52-408) membrane enzyme cargo and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsert2xCrk_SH3-mTurquoise2-CpPT1(52-408)
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A
Plasmid#238333PurposeproUBI10::VIH2-FL::FLAG-R372A-H373A-R376A ubiquitous expression in Arabidopsis plantsDepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-P378V-G500V-G501A
Plasmid#238338PurposeproUBI10::VIH2-FL::FLAG-P378V-G500V-G501A ubiquitous expression in Arabidopsis plantsDepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1469
Plasmid#239517PurposeExpress the upper portion of the melvaonate pathway enzymes (MvaE and MvaS) in the peroxisomeDepositorInsertEfMvaE-PTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-R722S-D724A-T726A
Plasmid#238339PurposeproUBI10::VIH2-FL::FLAG-R722S-D724A-T726A ubiquitous expression in Arabidopsis plantsDepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A
Plasmid#238340PurposeproUBI10::VIH2-FL::FLAG-K383A-K447A-K598A-R722A ubiquitous expression in Arabidopsis plantsDepositorInsertVIP1 (VIP1 Mustard Weed)
Tags3x Flag-tagExpressionPlantMutationAtVIH2-K383A-K447A-K598A-R722AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1461
Plasmid#239515PurposeExpress the lower portion of melvaonate pathway enzymes (Erg12p, Erg8p, and Erg19p) in the peroxisome; chromosomal integration by CRISPRDepositorInsertScErg12p-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1531
Plasmid#239521PurposeExpress truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPRDepositorInserttVoGES-ePTS1
ExpressionYeastAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A
Plasmid#238334PurposeproUBI10::VIH2-FL::FLAG-R372A-H373A-R376A-E882A ubiquitous expression in Arabidopsis plantsDepositorInsertVIP1 (VIP1 Mustard Weed)
Tags3x Flag-tagExpressionPlantMutationAtVIH2-R372A-H373A-R376A-E882AAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
proUBI10::VIH2-FL::FLAG-C696A
Plasmid#238336PurposeproUBI10::VIH2-FL::FLAG-C696A ubiquitous expression in Arabidopsis plantsDepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only