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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,023 results for: golden gate

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Showing: 4241 - 4260 of 5023 results

  7. pIF1079

    Plasmid
    #237445
    Purpose
    Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.
    Depositor
    IIya Finkelstein
    Article
    Buffington et al Nat Biotechnol. 2025 Oct 23. doi: 10.103
    Inserts
    SpCas9
    Efe1-RT
    EMX1 sgRNA
    Efe1-ncRNA targeting EMX1
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    CAG, H1, and U6
    Available Since
    June 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    237445-plasmid-map-sequence-id-476973

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  12. pCHW90004

    Plasmid
    #222436
    Purpose
    Level 0, promoter module (GGAG-CCAT) containing CBS4 and tomato miniDFR promoters.
    Depositor
    Chih-Hang Wu
    Article
    Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi
    Insert
    CBS4-miniDFR, CBS4 promoter + miniDFR minimal promoter (Solanum lycopersicum)
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    no
    Available Since
    Nov. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    222436-plasmid-map-sequence-id-452566

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  15. pCS2TAL3-DD

    Plasmid
    #37275
    Purpose
    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)
    Depositor
    David Grunwald
    Article
    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub
    Type
    Empty backbone
    Tags
    Flag, Fok I DD, Tal-C, and Tal-N
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    June 27, 2012
    Availability
    Academic Institutions and Nonprofits only
    37275-plasmid-map-sequence-id-46872

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  18. pGGG Wheat GUS marker

    Plasmid
    #165418
    Purpose
    Wheat transformation binary vector pGGG confers hygromycin resistance in wheat and contains the GUS (2x introns) reporter gene driven by OsUbi promoter
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Article
    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.
    Insert
    Os Ubiquitin Promoter::Gus (2x introns)::NosT
    Use
    Wheat transformation binary vector
    Promoter
    OS UbiP::GUS (2xint)::NosT
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165418-plasmid-map-sequence-id-331269

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Showing: 4241 - 4260 of 5023 results