We narrowed to 9,837 results for: pET
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Plasmid#204403PurposeE. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tagDepositorAvailable SinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only
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pET28-His8-aCat(660-906)
Plasmid#203333PurposeBacterial expression of alpha-catenin F-actin binding domainDepositorInsertCTNNA1 (CTNNA1 Human)
Tags8xHis with precision protease cleavage siteExpressionBacterialMutationaa 660-906 onlyAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-TEV-HsRCK_296-472_E
Plasmid#146209PurposeBacterial Expression of HsRck_296-472. Contains a TEV site rather than a HRV 3C site downstream of NusA.DepositorInsertHsRck_296-472 (DDX6 Human)
ExpressionBacterialAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(306-593)-6xhis
Plasmid#192837PurposeExpress codon-optimized human MIGA2 fragment 306-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags6xhisExpressionBacterialPromoterT7Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Arabidopsis thaliana Atm3 d80 pET21a+
Plasmid#173045PurposeExpresses AtAtm3 in E.coliDepositorInsertArabidopsis thaliana Atm3 (ABCB25 Mustard Weed)
Tags6xHis tagExpressionBacterialMutationwith N-terminal 80 amino acids deletionAvailable SinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
human KANK1 Ankyrin Repeats pET151
Plasmid#164053PurposeExpresses human KANK1 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
human KANK2 Ankyrin Repeats pET151
Plasmid#164054PurposeExpresses human KANK2 Ank domain in bacterial cellsDepositorInsertKidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
TagsHis-tag, TEV cleavage siteExpressionBacterialPromoterT7Available SinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RBP-GFP(S45C)-ZdK1
Plasmid#185791PurposeEncodes green flourescent protein linked to RNA binding peptide (on N terminal end ) and Z domain fragment (variant 1) of Staphylococcal protein A (on C-terminal) via GS-linkerDepositorInsertRBP-sfGFP(S45C)-ZdK1
Tags10xHis, TEV protease cleavage site after His TagExpressionBacterialMutationSer to Cys mutation at start of GFP, residue 45 i…PromoterT7Available SinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET24a His6 GST PYL2(14-188)
Plasmid#177842PurposeBacterial Expression of PYL2DepositorAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-SNAP-tag_cx
Plasmid#167273PurposeOverexpression of crystallizing SNAP-tag variant (P179R) in E. coliDepositorInsertSNAP-tag
TagsHis x10ExpressionBacterialMutationP179RPromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPV00605 pET21GG2-His(6)-BECN(1-265)
Plasmid#136686PurposeExpresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 IDR + CC (BECN1 Human)
TagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p230-pOsAct1::hpt-pZmUbi::BdPetC
Plasmid#129649PurposeRieske FeS overexpressionDepositorInsertCDS of PetC gene encoding for the Rieske FeS subunit
ExpressionPlantMutationCodon modified for Golden GatePromoterpZmUbiAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/L28F/G51PEKN
Plasmid#118585PurposeBacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, L28 mutated to F28, G51 mutat…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q109E (TAC-->TAT)
Plasmid#105735PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109E (TAC-->TAT) (SNCA Human)
ExpressionBacterialMutationQ109EPromoterT7Available SinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-ASXL1-HARE-HTH domain
Plasmid#107164PurposeExpresses murine ASXL1 HARE-HTH domain (amino acids 2-83) in E. coliDepositorInsertASXL1 (Asxl1 Mouse)
TagsFlagExpressionBacterialMutationContains amino acids 2-83PromoterT7Available SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-FgßF3-ddFLN4-HIS-ybbr
Plasmid#101743Purposemutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forcesDepositorInsertFgßF3 peptide, ddFLN4 fingerprint (FGB Human, Dictyostelium discoideum)
TagsHIS and ybbrExpressionBacterialMutationC18S in ddFLN4, added 9F in FgßF3PromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q109N (TAC-->TAT)
Plasmid#105737PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109N (TAC-->TAT) (SNCA Human)
ExpressionBacterialMutationQ109NPromoterT7Available SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q99E (TAC-->TAT)
Plasmid#105734PurposeBacterial expression of mutant human alpha synucleinDepositorAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only