We narrowed to 24,461 results for: pET

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pET28 Set9 mutant

Plasmid

#24083

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-FPPS

Plasmid

#108952

Purpose

heterologous expression of FPPS of G. stearothermophilus in E. coli

Depositor

Frank Schulz

Article

Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

Insert

Farnesyldiphosphat synthase (FPPS)

Tags

His6-Tag

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a_6HT-TBP6.7(R49A)

Plasmid

#112726

Purpose

Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

Depositor

Joseph Wedekind

Article

Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

Insert

6His-TEV-TBP6.7(R49A)

Tags

6His-TEV

Expression

Bacterial

Mutation

R49A

Promoter

T7

Available Since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2 A141S

Plasmid

#16157

Depositor

L. Miguel Martins

Article

Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

A141S

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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TrmD_HaeIn/pET28b(+) N,C-tag

Plasmid

#12665

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 200

Insert

tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)

Tags

His

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET His10-Src42a

Plasmid

#126674

Purpose

Expression of His10-Src42a for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Src42a

Expression

Bacterial

Available Since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-deltaPYD

Plasmid

#51535

Purpose

Express His6-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

His6 and T7

Expression

Bacterial

Promoter

lac operator

Available Since

March 10, 2014

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IS200

Plasmid

#12604

Depositor

SeWon Suh

Article

Lee et al J Biol Chem. 2006 Feb 17. 281(7):4261-6.

Insert

IS200 transposase (SSO1474 Sulfolobus solfataricus)

Expression

Bacterial

Available Since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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D5 gene in PETCON vector

Plasmid

#87626

Purpose

expresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast display

Depositor

Sarel Fleishman

Article

Khersonsky et al Protein Sci. 2017 Apr;26(4):807-813. doi

Insert

Designed allosteric Zn2+ and fluorescein binding antibody scFv D5

Tags

3x(G4S) Linker and cMyc tag on backbone

Expression

Yeast

Available Since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pET28-p53 (Mouse 1-87)

Plasmid

#62061

Purpose

Expression of Mouse p53 (residues 1-87) in e. coli

Depositor

Gary Daughdrill

Article

Daughdrill et al PLoS One. 2011;6(12):e29207. doi: 10.137

Insert

p53 (Trp53 Mouse)

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-87

Promoter

T7

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pET30Xa-SBL1(160S)

Plasmid

#66872

Purpose

Expresses WTsoybean lipoxygenase-1 160S with N-term Histag

Depositor

Betty Gaffney

Article

Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

Insert

soybean lipoxygenase-1 wild type (NEWENTRY Glycine max)

Tags

Histag from pET30Xa-LIC

Expression

Bacterial

Mutation

none

Promoter

T7lac

Available Since

Aug. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-GST

Plasmid

#51533

Purpose

Express GST-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available Since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET20b- hTPI Lys13Arg

Plasmid

#50726

Purpose

overexpression of the human TPI1 Lys13Arg allele in E.coli

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Tags

6xHIS tag

Expression

Bacterial

Mutation

changed Lysine 13 to Argenine

Promoter

T7

Available Since

Feb. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pET-MBP-creM

Plasmid

#112238

Purpose

pET28-derived plasmid that expresses an N-terminal maltose binding fusion construct of CreM

Depositor

Emily Balskus

Article

Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

Insert

CreM

Tags

MBP

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBC2T-Ypet-FtnA

Plasmid

#108969

Purpose

expresses BC2-tag-Ypet-FtnA in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

FtnA (ftnA Escherichia coli)

Tags

BC2-tag and Ypet

Expression

Mammalian

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pETM6-3F2-mCherry

Plasmid

#73418

Purpose

Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Promoter 3F2 (orthogonal T7-lac variant)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P3F2 (orthogonal T7-lac variant)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pET21b - S. thermophilus DltB

Plasmid

#132607

Purpose

expresses S. thermophilus DltB in E. coli

Depositor

Wenqing Xu

Article

Ma et al Nature. 2018 Oct;562(7726):286-290. doi:

Insert

Dltb

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET151-mUCH37 (ISF2)

Plasmid

#61928

Purpose

Expression of mUCH37 (ISF2) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

UCH37 (M.musculus) (Uchl5 Mouse)

Tags

6x HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pAR69_STRI_m(KS_MAT)_H8_pET22b

Plasmid

#122849

Purpose

Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

m(KS_MAT) (Fasn Mouse)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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