We narrowed to 10,183 results for: yeast

1. pGBT9-AAGAB

   Plasmid

   #198332

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AAGAB (AAGAB Human)
   

   Tags

   GAL4-DNA binding domain (BD) fragment

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROS13_IDH2 #7

   Plasmid

   #166102

   Purpose

   This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH2 sgRNA #7 (IDH2 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pROS13_IDH1 #4

   Plasmid

   #166101

   Purpose

   This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

   Depositor

   Matthias Heinemann

   Article

   Chen et al bioRxiv

   Insert

   IDH1 sgRNA #4 (IDH1 Budding Yeast)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT174_GalL_A3A-Y130F-BE3

   Plasmid

   #145123

   Purpose

   Expressing base editor A3A-Y130F-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(Y130F)-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(Y130F); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT173_GalL_A3A-R128A-BE3

   Plasmid

   #145122

   Purpose

   Expressing base editor A3A-R128A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(R128A)-BE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   A3A(R128A); spCas9(D10A)

   Promoter

   GalL

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJT113_GalL_cCDA1-xBE3

   Plasmid

   #145079

   Purpose

   Expressing base editor cCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-xBE3
   

   Use

   CRISPR

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZ_P-HXT1-FAS1up

   Plasmid

   #126746

   Purpose

   vector for replacing the promoter of FAS1 coding gene

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   fas1 upstream
   

   Tags

   6xHis

   Expression

   Yeast

   Promoter

   HXT1

   Available Since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNIA-CEN-FLAG-LINXa4-Bre1

   Plasmid

   #73617

   Purpose

   Control of the E3 ligase Bre1 with LINXa in yeast.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa4-Bre1dNLS
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A

   Promoter

   ADH1

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH394-SUP45-L34S

   Plasmid

   #29373

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   L34S mutant gene (corresponding to the sup45-36ts…

   Available Since

   May 23, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pTH401-SUP45-R65C

    Plasmid

    #29381

    Depositor

    Tobias von der Haar

    Article

    Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

    Insert

    SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    R65C mutant gene (corresponding to the sup45-3 al…

    Available Since

    May 23, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAV10

    Plasmid

    #63213

    Purpose

    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS426-HsFECH

    Plasmid

    #174524

    Purpose

    Expresses human ferrochelatase (FECH) in yeast

    Depositor

    Oleh Khalimonchuk

    Article

    Dietz et al Redox Biol. 2021 Oct;46:102125. doi: 10.

    Insert

    FECH with yeast HEM15 promoter and terminator (FECH Budding Yeast, Human, Synthetic)
    

    Expression

    Yeast

    Mutation

    Lacks original mitochondrial targeting sequence (…

    Promoter

    HEM15 (yeast, 257 bp)

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 1

    Pooled Library

    #168776

    Purpose

    Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Species

    Sars-cov-2

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

15. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library 2

    Pooled Library

    #168777

    Purpose

    Pooled library expressing barcoded SARS-CoV-2 RNA Binding Domain mutations

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Yeast

    Species

    Sars-cov-2

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

16. SARS-CoV-2 Spike (S) Ectodomain Library C

    Pooled Library

    #157973

    Purpose

    Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Bacterial and Yeast

    Species

    Sars-cov-2

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

17. SARS-CoV-2 Spike (S) Ectodomain Library B

    Pooled Library

    #157972

    Purpose

    Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Bacterial and Yeast

    Species

    Sars-cov-2

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

18. SARS-CoV-2 Spike (S) Ectodomain Library A

    Pooled Library

    #157971

    Purpose

    Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Expression

    Bacterial and Yeast

    Species

    Sars-cov-2

    Available Since

    Sept. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

19. pYTK001

    Plasmid

    #65108

    Purpose

    Part Plasmid Entry Vector to be used in the Dueber YTK system.

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    none
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. p406TDH3

    Plasmid

    #15977

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    TDH3 promoter + polylinker + CYC1 terminator (TDH3 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    TDH3 promoter is between SacI and XbaI restrictio…

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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