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pST_APOE_HUMAN_E114I (pBS0839)

Plasmid

#185274

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E114I. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E114I

Expression

Mammalian

Mutation

E114I

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pEPQD1CB0770

Plasmid

#177060

Purpose

MoClo Level 1, position 5, transcriptional unit for transient expression of loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus driven by 35S promoter; contains a chloroplast transit peptide

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase (CrLAMT) from Catharanthus roseus

Use

Synthetic Biology

Available Since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2-hFAM 3B-His

Plasmid

#172192

Purpose

Coding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned medium

Depositor

Edward De Robertis

Article

Zhang et al Proc Natl Acad Sci U S A. 2021 May 18;11

Insert

hFAM 3B

Tags

His

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_U2AF1_RRM

Plasmid

#110033

Purpose

Protein expression and purification of U2AF1_RRM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

U2AF1_RRM (U2AF1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GTF2F1_TFIIF

Plasmid

#110037

Purpose

Protein expression and purification of GTF2F1_TFIIF

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GTF2F1_TFIIF (GTF2F1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ABLIM1_LIM

Plasmid

#109880

Purpose

Protein expression and purification of ABLIM1_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ABLIM1_LIM (ABLIM1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1228

Plasmid

#136743

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1228

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1228

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Olfr1507

Plasmid

#136745

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Olfr1507

Depositor

Peter Mombaerts

Article

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Olfr1507

Use

In situ probe

Mutation

ISH Probe insert

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.VTN.C-HA

Plasmid

#154849

Purpose

Expresses murine Vitronectin (VTN) protein with HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Vitronectin (Vtn Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo*

Plasmid

#140165

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-DsRed-Express2-FKBP(LV)(C22V)

Expression

Insect

Promoter

hs70

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFA-eCA

Plasmid

#133010

Purpose

pFA carrying a E. coli canA gene (Uniprot: P61517)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

Can (can E. coli)

Tags

6xhis

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PE_28: Plux_lac_tet_eG::sfYFP

Plasmid

#104889

Purpose

PE_28: Plux_lac_tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Plux_lac_tet_eG

Expression

Bacterial

Promoter

Plux_lac_tet_eG::sfYFP

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-BE3-2X

Plasmid

#110835

Purpose

CMV expression vector for BE3-2X construct (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-2X

Expression

Mammalian

Mutation

2 tandem NLS sequences in the XTEN linker

Promoter

CMV

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Drd2

Plasmid

#105482

Purpose

to generate a riboprobe for in situ hybridazation

Depositor

Peter Mombaerts

Article

Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

Insert

Drd2 (Drd2 Mouse)

Use

To ganerate riboprobe

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_p.E63K

Plasmid

#82786

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway Cloning

Mutation

E63K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_p.R559P

Plasmid

#82789

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway Cloning

Mutation

R559P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_DCAF8_p.G588C

Plasmid

#82792

Purpose

Gateway Donor vector containing DCAF8, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

DCAF8 (DCAF8 Human)

Use

Gateway Cloning

Mutation

G588C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 xLabial

Plasmid

#24965

Depositor

Edward De Robertis

Article

Blumberg et al Science. 1991 Jul 12. 253(5016):194-6.

Insert

labial (hoxd1 Frog)

Expression

Mammalian

Available Since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
BK48

Plasmid

#1510

Depositor

Andrew Fire

Type

Empty backbone

Tags

T3p, let858-562, let858Tsa, let858N, gfpN, IVSR, …

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L3559

Plasmid

#1551

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, IVSA, gfpN, IVSR, gfpY66H, IV…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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