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Innate Immune Signaling Organelles Display Natural and Programmable Signaling Flexibility.
Tan Y, Kagan JC
Cell. 2019 Apr 4;177(2):384-398.e11. doi: 10.1016/j.cell.2019.01.039. Epub 2019 Mar 7.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
131345pLentiCRISPRv2-TRAF6-targeting sgRNA 1gRNA targeting mouse TRAF6
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131346pLentiCRISPRv2-TRAF6-targeting sgRNA 2gRNA targeting mouse TRAF6
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131347pMSCV-IRES-GFP-MyD88-NpLxISExpresses MyD88 with 2 copies of pLxIS motif from STING fused to its N-ternimus
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131348pMSCV-IRES-GFP-MyD88-CpLxISExpresses MyD88 with 2 copies of pLxIS motif from STING fused to its C-terminus
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131349pMSCV-IRES-GFP-MyD88 (S34Y)-CpLxISExpresses MyD88 S34Y with 2 copies of pLxIS motif from STING fused to its C-terminus
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131350pMSCV-IRES-GFP-3xFLAG-MyD88-GyrBExpresses MyD88 with an N-termial 3xFLAG tag and a C-terminal GyrB domain from the E. coli DNA Gyrase B
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131351pMSCV-IRES-GFP-3xFLAG-MyD88-V5Expresses MyD88 tagged with a 3xFLAG tag at its N-terminus and a V5 tag at its C-terminus
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131352pMSCV-IRES-GFP-MyD88-Rip3Expresses MyD88 with Rip3 fused to its C-terminus
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131353pMSCV-IRES-GFP-Caspase-1 (C285A)Expresses Caspase-1 C285A
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131354pMSCV-IRES-GFP-Caspase-1 (C285A) -NpLxISExpresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its N-ternimus
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131355pMSCV-IRES-GFP-Caspase-1 (C285A) -CpLxISExpresses Caspase-1 C285A with 2 copies of pLxIS motif from STING fused to its C-ternimus
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