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pcDNA3-FLAG-RIPK3 4KR

Plasmid

#78821

Purpose

To overexpress RIPK3 4KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pSR322

Plasmid

#125116

Purpose

Expression of uhpA(1-123aa)-ydfI(126-213aa), narL131 chimera under PLtetO-1, output Promoter PydfJ115

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

uhpA(REC)-ydfI(DBD)131
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.15

Plasmid

#125082

Purpose

Expression of ompR(1-137aa)-ccaR(127-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)137
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pSR40.29

Plasmid

#125088

Purpose

Expression of ompR(1-139aa)-ccaR(129-234aa) chimera under PLtetO-1, output Promoter PcpcG2-172

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

ompR(REC)-ccaR(DBD)139
sfgfp
tetR

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_129

Plasmid

#65738

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-129

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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pRRG319-nlk

Plasmid

#99082

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea nemo-like kinase (also known as h.109.4e)

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

nemo-like kinase (also known as h.109.4e) in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a_RBPMS_1_100

Plasmid

#65735

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-100

Promoter

T7

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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Mouse c-myc (exon I)

Plasmid

#8446

Depositor

Frederic Mushinski

Article

Weissinger et al Mol Cell Biol 1993 Apr;13(4):2578-85.

Insert

Mouse c-myc (exon I) (Myc Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
sg resistant gamma-tubulin

Plasmid

#104433

Purpose

For expressing gamma-tubulin in cells expressing γTUBULIN single guide RNA

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin 1 (TUBG1 Human)

Tags

None

Expression

Mammalian

Mutation

changed t bp28 to c; g bp30 to a; c bp33 to a; …

Available Since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1Xpress-BcrR1090A-HisB

Plasmid

#32507

Depositor

John Groffen, Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR (BCR )

Tags

HISB

Expression

Mammalian

Mutation

R 1090 to A

Available Since

Jan. 26, 2012

Availability

Academic Institutions and Nonprofits only
Bcr DH domain pGEX

Plasmid

#38179

Depositor

Nora Heisterkamp

Article

Cho et al Mol Cell Biol. 2007 Feb;27(3):899-911. E

Insert

BCR DH domain (BCR Human)

Tags

GST

Expression

Bacterial

Mutation

BCR DH domain

Available Since

Oct. 4, 2012

Availability

Academic Institutions and Nonprofits only
pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCfB3037(XI-5 MarkerFree)

Plasmid

#73277

Purpose

EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5 T538N

Plasmid

#60537

Purpose

Donor vector for human Pak5 T538N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

T538N

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pDONR223 PAK5 S573N

Plasmid

#60538

Purpose

Donor vector for human Pak5 S573N mutant

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Mutation

S573N

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_1_120

Plasmid

#65730

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 1-120

Promoter

T7

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-Tipin-L195A

Plasmid

#23116

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Tipin (TIPIN Human)

Tags

Flag

Expression

Mammalian

Mutation

changed Leucine 195 to Alanine

Available Since

Feb. 8, 2010

Availability

Academic Institutions and Nonprofits only
pet23a_RBPMS_20_101

Plasmid

#65733

Purpose

plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

His Tag

Expression

Bacterial

Mutation

deletion construct encoding amino acids 20-101

Promoter

T7

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-RIPK3 4KR

Plasmid

#78825

Purpose

To detect localization of RIPK3 4KR protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

GFP

Expression

Mammalian

Mutation

K55R, K89R, K363R, K501R

Promoter

CMV

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
CS2-Myc-mASUN

Plasmid

#47044

Purpose

Expression of tagged-mouse ASUN

Depositor

Laura Lee

Article

Jodoin et al Mol Biol Cell. 2012 Dec;23(24):4713-24.

Insert

Asunder (Asun Mouse)

Tags

Myc

Expression

Mammalian

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only