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pGEM_H1.0_HaloTag

Plasmid

#247483

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.0 with Halo tag

Depositor

Kazuhiro Maeshima

Publication

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.0 (H1F0) (H1-0 Human)

Use

CRISPR

Tags

HaloTag

Available since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i-Sec61β

Plasmid

#250997

Purpose

Expression of Sec61β with an N-terminal mScarlet tag

Depositor

Anthony Pedley

Publication

Sha et al Proc Natl Acad Sci U S A. 2026 Feb 3;123

Insert

SEC61β (SEC61B Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)

Plasmid

#233545

Purpose

A CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

MAFB (MAFB Human)

Use

Lentiviral

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pP4-C

Plasmid

#225097

Purpose

gfpUV reporter vector with a high performance adenine-dependent riboswitch

Depositor

Robert Batey

Publication

Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

Insert

synthetic riboswitch

Expression

Bacterial

Promoter

synthetic constitutive promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-mTagBFP2-Sec61B

Plasmid

#228136

Purpose

Expresses mTagBFP2-Sec61B in mammalian cells

Depositor

Noboru Mizushima

Publication

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

Sec61B (SEC61B Human)

Use

Retroviral

Tags

mTagBFP2

Expression

Mammalian

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-TSen

Plasmid

#209916

Purpose

A FRET biosensor for ADAM17 activity

Depositor

Michiyuki Matsuda

Publication

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TSen

Tags

ECFP and YPet

Expression

Mammalian

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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ApoE3_mCh-SspB (pBS1144)

Plasmid

#185326

Purpose

For the mammalian expression of the human protein ApoE3 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE4_mCh-SspB (pBS1143)

Plasmid

#185325

Purpose

For the mammalian expression of the human protein ApoE4 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE4

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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AfrLEA3m_mCh-2xFKBP (pBS1137)

Plasmid

#185321

Purpose

For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

AfrLEA3m

Expression

Mammalian

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_APLP2_Kunitz-BPTI

Plasmid

#110041

Purpose

Protein expression and purification of APLP2_Kunitz-BPTI

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

APLP2_Kunitz-BPTI (APLP2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GRB2_SH3

Plasmid

#110015

Purpose

Protein expression and purification of GRB2_SH3

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GRB2_SH3 (GRB2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR3C1_Hormone-recep

Plasmid

#110018

Purpose

Protein expression and purification of NR3C1_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR3C1_Hormone-recep (NR3C1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DNM2_Dynamin-N

Plasmid

#110008

Purpose

Protein expression and purification of DNM2_Dynamin-N

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DNM2_Dynamin-N (DNM2 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CBX5_Chromo-shadow

Plasmid

#109805

Purpose

Protein expression and purification of CBX5_Chromo-shadow

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CBX5_Chromo-shadow (CBX5 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTEI135

Plasmid

#126073

Purpose

Vector for expression of mitochondria targeted YFP1-10 (T203Y) in plant cell

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

YFP1-10

Expression

Plant

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS2276

Plasmid

#111894

Purpose

High Copy number plasmid containing Ple346 (NEFM MiniPromoter) insert.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple346 (NEFM Human)

Use

Unspecified

Promoter

Ple346 (NEFM MiniPromoter)

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-BE3-FNLS

Plasmid

#110836

Purpose

CMV expression vector for BE3-FNLS construct (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Tags

3X FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-P2A-Puro

Plasmid

#110838

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Publication

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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bactin:GFP-DCX

Plasmid

#105961

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Publication

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

DCX (DCX Human)

Tags

GFP

Expression

Bacterial

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ath5:mKate2-aPKC-CAAX

Plasmid

#105957

Purpose

transgenesis

Depositor

Caren Norden

Publication

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

aPKC CAAX

Tags

mKate2

Expression

Bacterial

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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