We narrowed to 5,339 results for: pcdna3.1

1. hHSF1 S303/7A

   Plasmid

   #118365

   Purpose

   This plasmid expresses human HSF1 S303A and S307A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A and S307A

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124434

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in Drosophila

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Expression

   Insect

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. HiBiT-FZD6

   Plasmid

   #195844

   Purpose

   Expresses human FZD6 with an N-terminal HiBiT; contains M345L

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD6 (FZD6 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   M345L

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CoV2-N-B.1.1.7

   Plasmid

   #177958

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (D3L, R203K, G204R, S235F) (N )
   

   Expression

   Mammalian

   Mutation

   D3L,R203K,G204R,S235F

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. hHSF1 RNAi resistant

   Plasmid

   #118347

   Purpose

   This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

   Depositor

   Lea Sistonen

   Article

   Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

   Insert

   hHSF1 (HSF1 Human)
   

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   RNAi resistance mutations (does not change amino …

   Available Since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. CoV2-N-P.1

   Plasmid

   #177959

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
   

   Expression

   Mammalian

   Mutation

   P80R,R203K,G204R

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY095

   Plasmid

   #84744

   Purpose

   Expresses huLbCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   GFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Opto-GPR146

   Plasmid

   #106047

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR146; Opto-GPR146; E1)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR146 (GPR146 Human, Bovine)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpyTag-RBD

   Plasmid

   #159999

   Purpose

   Expresses SARS-CoV-2 RBD (340-538) (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   SpyTag-SARS-CoV-2 RBD (340-538) (S SARS-CoV-2)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA 3.1 mitoAURKA

    Plasmid

    #157750

    Purpose

    Expression of AURKA targeted to mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    Aurora A without the first 30AA + MTS of CoxVIII

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. InsRA-TagBFP

    Plasmid

    #79791

    Purpose

    Insulin receptor isoform A with a inter-domain TagBFP tag at extracellular region (between furin-like domain and transmembrane domain). For imaging insulin receptor trafficing.

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    InsRA (INSR Human)
    

    Tags

    TagBFP extracellular

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pCDNA3-myc-his-BACH1 P47A

    Plasmid

    #17644

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    To generate the tumor-associated mutant, the prol…

    Available Since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-TdT

    Plasmid

    #126450

    Purpose

    Expresses human TdT in mammalian cells. (pTBL206)

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    TdT (DNTT Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. CCM2_wt_eGFP

    Plasmid

    #208876

    Purpose

    Express eGFP-CCM2 in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    WT sequence

    Available Since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-MED7

    Plasmid

    #15416

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED7 (MED7 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-c/o NL4-3 Env delta CT

    Plasmid

    #139285

    Purpose

    HIV-1 NL4-3 glycoprotein lacking cytoplasmic tail

    Depositor

    Jeremy Luban

    Article

    Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

    Insert

    codon optimized HIV-1 Env delta cytoplasmic tail
    

    Expression

    Mammalian

    Mutation

    deletion of cytoplasmic tail after residue 710

    Promoter

    CMV IE

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. FLAG-hIes2

    Plasmid

    #15355

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

    Insert

    PAP-1 binding protein (INO80B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

18. Opto-A2AR

    Plasmid

    #106066

    Purpose

    Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-A2AR (ADORA2A Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLAG-MED19

    Plasmid

    #15420

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

    Insert

    MED19 (Med19 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. h-Plekhm1_FLAG-pcDNA 3.1

    Plasmid

    #73593

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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