We narrowed to 8,386 results for: Dos

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pCR-U6-gRNA-miniCMV-Nluc

Plasmid

#192657

Purpose

A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Nluc

Use

Luciferase

Expression

Mammalian

Promoter

U6, miniCMV

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-INS-sg

Plasmid

#194719

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINS

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (INS Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-MAFB-sg

Plasmid

#194718

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFB

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1 (MAFB Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV:AsCpf1-2A-GFP-U6-GFP-sg

Plasmid

#194717

Purpose

Bicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target GFP

Depositor

Rudolf Jaenisch

Article

Ma et al Bio Protoc. 2024 Nov 20;14(22):e5108. do

Insert

AsCpf1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mRuby3

Plasmid

#191744

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mRuby3

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_mNeptune2.5

Plasmid

#191748

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Article

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and mNeptune2.5

Expression

Bacterial

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL1RN gRNA_1-MS2-Puro

Plasmid

#192677

Purpose

Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNA #1 (IL1RN Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pBEL2039

Plasmid

#139929

Purpose

Complementation plasmid for LetAB without LetB TM region

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(43-877)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL2005

Plasmid

#139880

Purpose

Complementation plasmid for LetAB with 2 ring (rings17) LetB mutant

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Expression

Bacterial

Mutation

LetA-LetB(delta161-747)

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1993

Plasmid

#139897

Purpose

Expression plasmid for LetB PLL1 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta114-128, P113G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1999

Plasmid

#139903

Purpose

Expression plasmid for LetB PLL7 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Expression

Bacterial

Mutation

LetB(43-877; delta816-826, S815G, T827G)-6xHis

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD122

Plasmid

#172963

Purpose

pRMCD77 encoding Wzz mutant I35C/M32T

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz I35C/M32T mutant gene

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD113

Plasmid

#172959

Purpose

pRMCD77 encoding Wzz mutant G305A/G311A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G311A mutant gene

Expression

Bacterial

Mutation

G305A/G311A, A208V - please see depositor comments

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD112

Plasmid

#172958

Purpose

pRMCD77 encoding Wzz mutant G305A/G309A

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri wzz G305A/G309A mutant gene

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRMCD78

Plasmid

#172954

Purpose

pRMCD77 with S. flexneri 2a wzzBSF gene

Depositor

Renato Morona

Article

Daniels et al Mol Microbiol. 1999 Oct;34(1):181-94. do

Insert

Shigella flexneri 2a wzzBSF gene

Expression

Bacterial

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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U6-U57A

Plasmid

#169927

Purpose

WT U6 with a U57A mutation

Depositor

Aaron Hoskins

Article

van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

Insert

U6 U57A

Expression

Bacterial and Yeast

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pKP1064

Plasmid

#167607

Purpose

Express ExbD D25N protein

Depositor

Kathleen Postle

Article

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbD D25N

Expression

Bacterial

Mutation

exbD with D25N mutation

Available Since

July 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKP999

Plasmid

#160816

Purpose

Expression of ExbD protein

Depositor

Kathleen Postle

Article

Ollis et al Mol Microbiol. 2009 Aug;73(3):466-81. do

Insert

exbD

Expression

Bacterial

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD95

Plasmid

#158617

Purpose

CckA FRET Sensor

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3

Expression

Bacterial

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSWD55

Plasmid

#158618

Purpose

Short Fibronectin FRET standard

Depositor

Seth Childers

Article

Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

Insert

Short FRET Standard

Expression

Bacterial

Promoter

Xylose

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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