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pCT5-bac2.0

Plasmid

#119872

Purpose

Cumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coli

Depositor

Claudia Schmidt-dannert

Publication

Seo et al Appl Microbiol Biotechnol. 2018 Nov 3. p

Inserts

CymR
sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pveg promoter with CuO sequence and PxylR promoter

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Fuw-dCas9-dead Tet1CD-P2A-BFP

Plasmid

#108246

Purpose

Lentiviral vector to express dCas9-dead Tet1CD-P2A-tagBFP

Depositor

Rudolf Jaenisch

Publication

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

dCas9-dead Tet1CD-HA-P2A-BFP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

dCas9-D10A, dCas9-H840A, Tet1CD-H1672Y, Tet1CD-D1…

Promoter

Ubc

Available since

June 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pC0039-CMV-dPspCas13b-GS-ADAR2DD(E488Q)

Plasmid

#103849

Purpose

dPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA.

Depositor

Feng Zhang

Publication

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

PspCas13b
huADAR2DD (ADARB1 Human)

Use

CRISPR

Tags

GS and HIV NES

Expression

Mammalian

Mutation

Changed histidne 133 to alanine and histidine 105…

Promoter

CMV

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-hsyn-Jaws-KGC-GFP-ER2 (AAV8)

Viral prep

#65014-AAV8

Purpose

Ready-to-use AAV8 particles produced from pAAV-hsyn-Jaws-KGC-GFP-ER2 (#65014). In addition to the viral particles, you will also receive purified pAAV-hsyn-Jaws-KGC-GFP-ER2 plasmid DNA. hSyn-driven Jaws-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.

Depositor

Edward Boyden

Publication

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Promoter

Syn

Tags

GFP

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only
lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Publication

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mScarlet-I-mTurquoise2

Plasmid

#98839

Purpose

Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Publication

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mScarletI-mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCDNA3.1 (-) + FLAG-NLS-MS2-eGFP

Plasmid

#86827

Purpose

Expresses a nuclear localized, FLAG-tagged MS2-GFP fusion protein in mammalian cells, used for purification or localization of tagged RNAs

Depositor

Carl Novina

Publication

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

FLAG-NLS-MS2-eGFP

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pcDNA3-TDsmURFP-IRES-HO-1

Plasmid

#80346

Purpose

Expresses Tandem Dimer smURFP and Heme Oxygenase-1 in mammalian cells.

Depositor

Erik Rodriguez, Roger Tsien

Publication

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Tandem Dimer smURFP
HO-1

Expression

Mammalian

Promoter

CMV and IRES

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-RIPK3

Plasmid

#78804

Purpose

To overexpress RIPK3 in Mammalian Cells

Depositor

Jaewhan Song

Publication

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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Orai1-GCaMP6f

Plasmid

#73564

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Publication

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Orai1-GCaMP6f (ORAI1 Human, Synthetic)

Tags

GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA GNSTM-3-Flag-10-Lamp2b-HA

Plasmid

#71293

Purpose

Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

Depositor

Joshua Leonard

Publication

Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

Insert

Lamp2b (LAMP2 Human)

Tags

FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

Expression

Mammalian

Promoter

CMV

Available since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Publication

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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RGS-6xHis-pcDNA3.1-

Plasmid

#52534

Purpose

enables eukaryotic expression of N-terminally RGS-6xHis–tagged fusion proteins

Depositor

Adam Antebi

Publication

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Type

Empty backbone

Tags

RGS-6xHis

Expression

Mammalian

Promoter

CMV

Available since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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phage ubc nls ha 2xmcp HALO

Plasmid

#64540

Purpose

Used to label reporter mRNAs. Lentiviral expression of the MCP protein fused to HALO.

Depositor

Jeffrey Chao

Publication

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

MCP

Use

Lentiviral

Tags

HA, HALO, and NLS

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCS2-3xFLAG-NLS-SpCas9-NLS

Plasmid

#51307

Purpose

Cas9 expression plasmid

Depositor

Yonglong Chen

Publication

Guo et al Development. 2014 Jan 8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

3xFLAG-NLS-SpCas9-NLS

Use

CRISPR; Xenopus tropicalis expression

Tags

3x Flag and NLS

Promoter

CMV

Available since

Feb. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

Plasmid

#44027

Depositor

Celeste Simon

Publication

Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)

Tags

Myc

Expression

Mammalian

Mutation

P405A, P530V, N851A

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA3 tdAPX W41F

Plasmid

#40863

Purpose

Tandem dimer of pea ascorbate peroxidase (W41F mutant). This construct has been replaced by APEX2 (construct 49386).

Depositor

Alice Ting

Publication

Martell et al Nat Biotechnol. 2012 Oct 21. doi: 10.103

Insert

tandem dimer pea APX W41F

Tags

Flag

Expression

Mammalian

Mutation

W41F mutation

Promoter

CMV

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-VSFP-CR

Plasmid

#40257

Depositor

Michael Lin

Publication

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VSFP-CR

Expression

Mammalian

Promoter

CAG

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Publication

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles
FLAG-Pol-IIΔ

Plasmid

#35176

Depositor

Benjamin Blencowe

Publication

Rosonina et al RNA. 2004 Apr;10(4):581-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RPBI (POLR2A Human)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the entire C-terminal domain (repeats…

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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