We narrowed to 1,190 results for: pFAST

1. pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

   Plasmid

   #79036

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Changed Lys 283 to Asp and Gln 287 to Asp

   Promoter

   polyhedrin

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFastBac HTB His-TEV-rDPP9(S729A)-FLAG

   Plasmid

   #217329

   Purpose

   Purification of catalytically-dead rat DPP9 fusion with C-terminal FLAG tagfrom insect cells

   Depositor

   Yifan Wang

   Insert

   DPP9
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S729A

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFastBac HTB His-TEV-BN-rNLRP1(N61K)-FLAG

   Plasmid

   #217325

   Purpose

   Purification of Brown Norway rat NLRP1 (N61K mutation) fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   N61K

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

   Plasmid

   #217324

   Purpose

   Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   S969A

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

   Plasmid

   #217320

   Purpose

   Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

   Depositor

   Yifan Wang

   Insert

   NLRP1
   

   Use

   Tags

   FLAG and His-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

   Plasmid

   #55218

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

   Plasmid

   #30116

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6-MBP-N10-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

   Plasmid

   #198035

   Purpose

   Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   SINTBAD (TBKBP1 Human)
   

   Use

   Tags

   EGFP and GST-TEV

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

   Plasmid

   #223778

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BNIP3 soluble part (BNIP3 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Insect

   Mutation

   W18A/L21A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

    Plasmid

    #217321

    Purpose

    Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    S969A

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

    Plasmid

    #217013

    Purpose

    Expression of CDK7 in insect cells

    Depositor

    Manjeet Mukherjee

    Article

    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

    Insert

    CDK7(W132R, S164D, T170E) (CDK7 Human)
    

    Use

    Tags

    TEV cleavable his tag

    Expression

    Insect

    Mutation

    CDK7(W132R, S164D, T170E)

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid

    #79030

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    deleted amino acid 294-515 from frican green monk…

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)

    Plasmid

    #55218

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac His6 MBP N10 TEV LIC cloning vector (4C)

    Plasmid

    #30116

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFastBac_Dual-GST-TEV-SINTBAD-EGFP (codon optimized Sf9)

    Plasmid

    #198035

    Purpose

    Used for the expression and purification of EGFP labelled SINTBAD (SMC Internal No. 1613)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    SINTBAD (TBKBP1 Human)
    

    Use

    Tags

    EGFP and GST-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (W18A/L21A; deltaLIR)

    Plasmid

    #223778

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    BNIP3 soluble part (BNIP3 Human)
    

    Use

    Tags

    Thrombin cleavage-GST

    Expression

    Insect

    Mutation

    W18A/L21A; deletion of LIR

    Promoter

    Available since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

    Plasmid

    #217321

    Purpose

    Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Use

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Mutation

    S969A

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac dual_Human full length CDK7(W132R, S164D, T170E)

    Plasmid

    #217013

    Purpose

    Expression of CDK7 in insect cells

    Depositor

    Manjeet Mukherjee

    Article

    Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

    Insert

    CDK7(W132R, S164D, T170E) (CDK7 Human)
    

    Use

    Tags

    TEV cleavable his tag

    Expression

    Insect

    Mutation

    CDK7(W132R, S164D, T170E)

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid

    #79030

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    deleted amino acid 294-515 from frican green monk…

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

    Plasmid

    #30123

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge