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Combinatorial assembly of clone libraries using site-specific recombination.
Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D
Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
161878pDest-623 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system
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161879pDest-624 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system
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161880pDest-639 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for expression of multiple proteins using the Bac-to-Bac insect cell system
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161882pDest-658 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for lentivirus production
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161883pDest-659 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for lentivirus production
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161884pDest-663 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for lentivirus production
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161885pDest-665 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for lentivirus production
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161886pDest-667 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for lentivirus production
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161887pDest-669 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for lentivirus production
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161888pDest-676 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for lentivirus production
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161889pDest-686 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for lentivirus production
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161893pDest-894 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coli
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161894pDest-302 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for mammalian protein expression
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161895pDest-305 (attR4-attR2)Multisite Gateway (attR4-attR2) vector for mammalian protein expression
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161896pDest-311 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for mammalian protein expression
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161897pDest-312 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for mammalian protein expression
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161898pDest-313 (attR4-attR3)Multisite Gateway (attR4-attR3) vector for mammalian protein expression
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162888C121-E01: rLucStandard Gateway entry clone encoding Renilla luciferase
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162889C121-E02: ffLuc2Standard Gateway entry clone encoding firefly luciferase
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162890C121-E06: gLucStandard Gateway entry clone encoding Gaussia luciferase
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162891C121-E12: NanoLucStandard Gateway entry clone encoding NanoLuc luciferase
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162892C122-E10: mCherryStandard Gateway entry clone encoding mCherry fluorescent protein
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162893C122-E11: eGFPStandard Gateway entry clone encoding eGFP fluorescent protein
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162894C122-E14: mKateStandard Gateway entry clone encoding mKate fluorescent protein
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162895C122-E15: mOrangeStandard Gateway entry clone encoding mOrange fluorescent protein
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162896C122-E16: CyPetStandard Gateway entry clone encoding CyPet fluorescent protein
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162897C122-E17: mPlumStandard Gateway entry clone encoding mPlum fluorescent protein
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162898C122-E53: mClover3Standard Gateway entry clone encoding mClover fluorescent protein
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162899C122-E54: mRuby3Standard Gateway entry clone encoding mRuby3 fluorescent protein
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162900C125-E01: stufferStandard Gateway entry clone containing a non-transcribed/translated region for use in generating negative control vectors
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162901C231-E02: attB2r-NanoLuc-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal NanoLuc fusion proteins
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162902C232-E05: attB2r-meGFP-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal eGFP fusion proteins
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162903C232-E06: attB2r-mCherry-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins
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162904C232-E08: attB2r-mTFP1-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins
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162905C232-E25: attB2r-mVenus-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteins
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162906C232-E29: attB2r-mClover3-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal mClover3 fusion proteins
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162907C232-E30: attB2r-mRuby3-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteins
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162908C232-E34: attB2r-iRFP670-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal iRFP670 fusion proteins
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162909C234-E05: attB2r-V5-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal V5 epitope tagged fusion proteins
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162910C234-E06: attB2r-FLAG-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal FLAG epitope tagged fusion proteins
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162911C234-E07: attB2r-HA-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal HA epitope tagged fusion proteins
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162912C234-E08: attB2r-3xFLAG-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal triple-FLAG epitope tagged fusion proteins
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162913C234-E24: attB2r-Strep2-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal Strep2 tagged fusion proteins
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162914C234-E25: attB2r-SNAP-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal SNAP tagged fusion proteins
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162915C234-E26: attB2r-Halotag-attB3Gateway attB2r/attB3 entry clone for construction of C-terminal Halotag fusion proteins
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162916C238-E02: attB2r-BGHpolyA-attB3Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination
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162917C238-E07: attB2r-SV40polyA-attB3Gateway attB2r/attB3 entry clone containing a polyA sequence for transcription termination
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162918C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)
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162919C239-E03: attB2r-IRES-ffLuc2-pA-attB3Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2 reporter using an internal ribosome entry sequence (IRES)
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162920C413-E15: attB4-EF1p>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter
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162921C413-E21: attB4-PGKp>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter
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162922C413-E22: attB4-polyhedrinp>-attB1rGateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter
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162923C413-E26: attB4-SV40p>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the SV40 promoter
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162924C413-E31: attB4-TRE3Gp>-attB1rGateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoter
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162925C413-E33: attB4-UbCp>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the human Ubiquitin C promoter
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162926C413-E34: attB4-mCMVp>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the minimal CMV promoter
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162927C413-E36: attB4-CMV51p>-attB1rGateway attB4/attB1r entry clone for mammalian expression using an optimized human CMV promoter
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162928C413-E40: attB4-EFSp>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the short EF1 promoter
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162929C413-E38: attB4-T7p>-attB1rGateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter
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162930C413-E45: attB4-hr5-ie1p>-attB1rGateway attB4/attB1r entry clone for transient insect expression using the ie1 promoter from AcMNPV
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162931C415-E01: attB4-stuffer-attB1rGateway attB4-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors
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162932C417-E01: attB4-polyhedrinp>His6-nostop-attB1rGateway attB4/attB1r entry clone for baculovirus expression of His6 tagged proteins using the polyhedrin promoter
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162933C417-E02: attB4-polyhedrinp>His6-MBP-nostop-attB1rGateway attB4/attB1r entry clone for baculovirus expression of His6-MBP tagged proteins using the polyhedrin promoter
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162934C417-E03: attB4-polyhedrinp>His6-GST-nostop-attB1rGateway attB4/attB1r entry clone for baculovirus expression of His6-GST tagged proteins using the polyhedrin promoter
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162935C417-E06: attB4-polyhedrinp>MBP-nostop-attB1rGateway attB4/attB1r entry clone for baculovirus expression of MBP tagged proteins using the polyhedrin promoter
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162936C417-E10: attB4-polyhedrinp>3xFLAG-nostop-attB1rGateway attB4/attB1r entry clone for baculovirus expression of 3xFLAG tagged proteins using the polyhedrin promoter
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162937C417-E12: attB4-polyhedrinp>Strep2x2-nostop-attB1rGateway attB4/attB1r entry clone for baculovirus expression of dual Strep2 tagged proteins using the polyhedrin promoter
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162938C511-E02: attB5r-NanoLuc-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins
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162939C511-E15: attB5r-ffluc2-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal Nanoluciferase fusion proteins
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162940C512-E01: attB5r-meGFP-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal eGFP fusion proteins
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162941C512-E04: attB5r-mCherry-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal mCherry fusion proteins
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162942C512-E11: attB5r-mTFP1-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal mTFP1 fusion proteins
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162943C512-E15: attB5r-mVenus-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal mVenus fusion proteins
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162944C512-E22: attB5r-mClover3-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal mClover3 fusion proteins
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162945C512-E23: attB5r-mRuby3-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteins
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162946C512-E29: attB5r-mKate2-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal mKate2 fusion proteins
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162947C512-E34: attB5r-iRFP670-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal iRFP670 fusion proteins
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162948C513-E06: attB5r-pA-polyhedrinp>-attB1rGateway attB5r/attB1r entry clone containing an AcMNPV polyhedrin promoter with upstream polyA, for use in construction of polycistronic baculovirus expression vectors
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162949C513-E07: attB5r-<p10p-polyhedrinp>-attB1rGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors
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162950C513-E08: attB5r-<p10p-polyhedrinp>H6-MBP-nostop-attB1rGateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors; allows His6-MBP fusion for the polyhedrin transcribed protein
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162951C514-E09: attB5r-RU5u-FLAG-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins
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162952C514-E10: attB5r-RU5u-HA-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal HA-tagged fusion proteins
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162953C514-E11: attB5r-RU5u-His6-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins
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162954C514-E12: attB5r-RU5u-His6-eGFP-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal eGFP-tagged fusion proteins
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162955C514-E13: attB5r-RU5u-His6-GST-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins
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162956C514-E14: attB5r-RU5u-His6-Halotag-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins
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162957C514-E15: attB5r-RU5u-His6-MBP-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal FLAG-tagged fusion proteins
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162958C514-E23: attB5r-RU5u-3xFLAG-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal 3xFLAG-tagged fusion proteins
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162959C514-E24: attB5r-RU5u-V5-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal V5-tagged fusion proteins
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162960C514-E26: attB5r-RU5u-myc-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal Myc-tagged fusion proteins
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162961C514-E42: attB5r-RU5u-Strep2-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal Strep2-tagged fusion proteins
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162962C514-E44: attB5r-SNAP-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal SNAP-tagged fusion proteins
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162963C514-E47: attB5r-Halotag-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal Halotag-tagged fusion proteins
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162964C514-E51: attB5r-RBS-His6-MBP-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal His6-MBP-tagged fusion proteins in bacterial expression
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162965C514-E54: attB5r-RBS-His6-Halotag-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal His6-Halotag-tagged fusion proteins in bacterial expression
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162966C514-E59: attB5r-RU5u-2xHA-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal dual HA-tagged fusion proteins
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162967C514-E67: attB5r-RU5u-Strep2x2-nostop-attB1rGateway attB5r/attB1r entry clone for generation of N-terminal dual Strep-tagged fusion proteins
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162968C515-E01: attB5r-stuffer-attB1rGateway attB5r-attB1r entry clone containing a non-transcribed/translated region for use in generating negative control vectors
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162969C413-E10: attB4-FerHp>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter
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162970C413-E11: attB4-Grp78p>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter
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162971C413-E19: attB4-mPol2p>-attB1rGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoter
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162972C453-E02: attB4-CAGp>-attB5Gateway attB4/attB5 entry clone for mammalian expression using the CAG promoter
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162973C453-E04: attB4-CMV51p>-attB5Gateway attB4/attB5 entry clone for mammalian expression using an optimized human CMV promoter
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162974C453-E09: attB4-Grp78p>-attB5Gateway attB4/attB5 entry clone for mammalian expression using the human Grp78 promoter
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162975C453-E14: attB4-EF1p>-attB5Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter
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162976C453-E17: attB4-PGKp>-attB5Gateway attB4/attB5 entry clone for mammalian expression using the human PGK promoter
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162977C453-E18: attB4-polyhedrinp>-attB5Gateway attB4/attB5 entry clone for baculovirus expression using the AcMNPV polyhedrin promoter
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162978C453-E21: attB4-FerHp>-attB5Gateway attB4/attB5 entry clone for mammalian expression using the human EF1alpha promoter
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162979C453-E23: attB4-TRE3Gp>-attB5Gateway attB4/attB5 entry clone for inducible mammalian expression using the TRE3G promoter
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162980C453-E24: attB4-UbCp>-attB5Gateway attB4/attB5 entry clone for mammalian expression using the human Ubiquitin C promoter
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162981C453-E25: attB4-mPol2p>-attB5Gateway attB4/attB5 entry clone for mammalian expression using the murine RNA polymerase 2 promoter
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162982C453-E26: attB4-T7p>-attB5Gateway attB4/attB1r entry clone for bacterial expression using the bacteriophage T7 promoter
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162983C455-E01: attB4-stuffer-attB5Gateway attB4-attB5 entry clone containing a non-transcribed/translated region for use in generating negative control vectors
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Antibodies from Article