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pEGFP-C1-PHF13

Plasmid

#246093

Purpose

Expression of human PHF13 fused to EGFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

EGF

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV4-PHF13

Plasmid

#246094

Purpose

Expression of human PHF13 with Flag-tag in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (PHF13 Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1α-H2B-HaloTag-FRT

Plasmid

#247338

Purpose

Expresses H2B-Halo under EF1 promoter and can be integrated into FRT site

Depositor

Kazuhiro Maeshima

Article

Nozaki et al Mol Cell. 2017 Jul 20;67(2):282-293.e7.

Insert

H2B (H2BC11) (H2BC11 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-Rubicon-CGHL

Plasmid

#221658

Purpose

Rubicon mutated at four residues in the RH domain to disrupt Rab7 binding, tagged at the N-terminus with mCherry

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

mCherry

Expression

Mammalian

Mutation

C912G, C915G, H920L, C923G

Promoter

CMV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AAV Syn-FLEX-coca-GlyR-IRES-mCherry

Plasmid

#242196

Purpose

Cre-dependent AAV vector for cocaine-gated chemogenetic anion channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

Syn-FLEX-coca-GlyR-IRES-mCherry

Use

AAV

Expression

Mammalian

Mutation

L141G G175K Y217F

Promoter

Synapsin

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pME-DRE-nls (JDW 31)

Plasmid

#242561

Purpose

Gateway middle entry clone containing Dre recombinase

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

codon optimized DRE-nls

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-SV40-pA (JDW 1417)

Plasmid

#242570

Purpose

Gateway 3' entry clone containing mCherry followed by an SV40 polyA.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCherry stop SV40 pA

Use

Gateway subcloning

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-G12-AkaLuc-P2A-Proinsulin

Plasmid

#241776

Purpose

Tested on HeLa for bioluminescence, and used for production of AAV G12-Akaluc-P2A-Proinsulin for mice.

Depositor

Vladislav Verkhusha

Article

Kasatkina et al Nat Commun. 2025 Jul 14;16(1):6469. doi:

Insert

AkaLuc-P2A-Proinsulin

Use

AAV

Promoter

12xUAS with TATA-box minimal promoter

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-mRuby2-3xMyc (JDW 1246)

Plasmid

#242554

Purpose

Gateway middle entry clone containing Lifeact-mRuby2-3xMyc; Red F-actin reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mRuby2

Use

Gateway subcloning

Available Since

Sept. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-R-eLACCO2

Plasmid

#223255

Purpose

Biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

eLACCO2.1

Expression

Bacterial

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-R-eLACCO2.1

Plasmid

#223256

Purpose

Control biosensor for extracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

deLACCO1

Expression

Bacterial

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD-R-deLACCOctrl

Plasmid

#223257

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell, Yusuke Nasu

Article

Kamijo et al Nat Commun. 2025 Oct 29;16(1):9531. doi:

Insert

R-iLACCO1

Expression

Bacterial

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pME-H2B-mScarlet-3H (JDW 1513)

Plasmid

#242551

Purpose

Gateway middle entry clone containing H2B-mScarlet-3H; Nuclear red fluorescent reporter

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mScarlet3-H

Use

Gateway subcloning

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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ubbr:GCaMP8m

Plasmid

#232485

Purpose

To make fish transgenic lines that have calcium sensor expression in the whole body of the fish

Depositor

Chie Satou

Article

Ruetten et al bioRxiv 2025.08.20.670374

Insert

jGCaMP8m

Use

Zebrafish expression

Promoter

ubbr

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pLV EF1alpha INTS4 IRES Cherry

Plasmid

#232109

Purpose

Lentiviral expression of human INTS4

Depositor

Tom Misteli

Article

Sood et al Mol Cell. 2025 Mar 6;85(5):913-928.e8. d

Insert

INTS4 (INTS4 Human)

Use

Lentiviral

Tags

IRES CHERRY

Expression

Mammalian

Mutation

Wild Type

Promoter

EF 1alpha

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-TRIP12(749-836)-EGFP

Plasmid

#196229

Purpose

EGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged TRIP12(749- 836)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-DTX2(8-174)-EGFP

Plasmid

#196232

Purpose

EGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

DTX2(8-174)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP

Plasmid

#196235

Purpose

EGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(K35E/Y145R)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

K35E and Y145R

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-RNF146(100-182)-myc

Plasmid

#196236

Purpose

RNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(100-182) encoding a C-terminal myc tag

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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M7138

Plasmid

#219774

Purpose

MoClo-compatible Level M vector encoding hygromycin resistance cassette and a luciferase gene under control of ORCA3 promoter and terminator

Depositor

Karen Sarkisyan

Article

Balakireva et al bioRxiv 2025.02.17.636591; doi: https://

Insert

pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T

Use

Synthetic Biology

Expression

Plant

Promoter

pNOS-HygR-ocsT | pORCA3-nnLuz-ORCA3_T

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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