We narrowed to 16,007 results for: grn

1. pLKO1-puro-U6-sgRNA-TGM2

   Plasmid

   #69239

   Purpose

   U6 driven SpCas9 sgRNA expression for TGM2 site

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

   Insert

   TGM2 sgRNA (TGM2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pS23.U6<ActB.mus_C-term-KI_gRNA

   Plasmid

   #207408

   Purpose

   gRNA from a U6 promoter targeting the mouse ActB near the 3' end of the ORF. Used for C-terminal knockin by DSB repair. [Lab plasmid ID: TU260]

   Depositor

   Alexandros Poulopoulos

   Article

   Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

   Insert

   ActB
   

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Nov. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAC-U63-tgRNA-nlsBFP

   Plasmid

   #169029

   Purpose

   gRNA-marker vector contain a U6:3 promoter, gRNA scaffold, and a Ubi-mTagBFP-NLS marker.

   Depositor

   Chun Han

   Article

   Allen et al PLoS Biol. 2021 Jan 14;19(1):e3001061. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6:3

   Available Since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBbdCas9S(-10AC12)_Psyn-sgRNArodA

   Plasmid

   #149661

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi rodA

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNArodA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30(-10AC12), Psyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSico_U6-Pan-PGC1a sgRNA

   Plasmid

   #165426

   Purpose

   Expression of gRNA against human Total PGC-1a variants

   Depositor

   Antonio Amelio

   Article

   Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:

   Insert

   gRNA against human Total PGC-1a variants (PPARGC1A Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX601‐mhCMV‐ABEmaxNGA‐C3‐ E53ogRNA

   Plasmid

   #187063

   Purpose

   Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   Npu Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutation
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mhCMV

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6-CB2gRNA-CBh-mCherry

   Plasmid

   #91948

   Purpose

   Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

   Depositor

   Jimok Kim

   Article

   Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AeU6 3p3 GFP LgRNA

   Plasmid

   #182208

   Purpose

   For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegypti

   Depositor

   Craig Montell

   Article

   Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

   Insert

   3xP3-GFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBbdCas9S(RBSmut)_Psyn-sgRNAflaB

   Plasmid

   #149588

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi flaB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAflaB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, Psyn

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKLV2-U6gRNA5(Nelfb-g1)-PGKpuroBFP-W

    Plasmid

    #105023

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNA5(Nelfb-g2)-PGKpuroBFP-W

    Plasmid

    #105024

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfb , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfb (Cobra1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

    Plasmid

    #105025

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

    Plasmid

    #105026

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9-Puro HLAG-2B-sgRNA

    Plasmid

    #182552

    Purpose

    Cas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G gene

    Depositor

    Santiago Miriuka

    Article

    Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

    Insert

    hSpCas9-2A-Puro-HLAG-2B-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBbdCas9S(RBSmut)_Psyn-sgRNAftsI

    Plasmid

    #149590

    Purpose

    all-in-one CRISPRi vector for targeting B. burgdorferi ftsI

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

    Insert

    dCas9, lacI, sgRNAftsI
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PflaB, PpQE30, Psyn

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pM116: CasRx VEGFA presgRNA

    Plasmid

    #166868

    Purpose

    U6-driven expression of human VEGFA targeting presgRNA compatible with CasRx.

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    Human VEGFA targeting presgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT7-SpCas9_sgRNA-site2 (RTW448)

    Plasmid

    #160137

    Purpose

    T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available Since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. tRNA-gRNA position [n] (GB1207)

    Plasmid

    #75408

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [n]
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAN-PBAD-sgRNA-scramble

    Plasmid

    #62285

    Purpose

    expression of scramble sgRNA from the arabinose-inducible promoter

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    scramble sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLKO.1-puro U6 sgRNA SOX17 -91

    Plasmid

    #50923

    Purpose

    U6 driven sgRNA targeting Sox17 -91 bp from TSS

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Sox17 -91 sgRNA (SOX17 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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