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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

Plasmid

#166798

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

LL100EE

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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mKate1.3-Fibrillarin-7

Plasmid

#56084

Purpose

Localization: Nucleoli, Excitation: 588, Emission: 635

Depositor

Michael Davidson

Article

Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

Insert

Fibrillarin (FBL Human)

Tags

mKate1.3 (aka mKate2)

Expression

Mammalian

Mutation

R36Q, S66F

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSICO-CPSF6-iRFP670

Plasmid

#167586

Purpose

Expresses human CPSF6 fused to iRFP

Depositor

Zandrea Ambrose

Article

Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

Insert

CPSF6-iRFP670 (CPSF6 Human)

Use

Lentiviral

Tags

iRFP670

Mutation

WT

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-H1-shRNA-CMV-HA-PACSIN1

Plasmid

#72577

Purpose

Bicistronic construct expressing PACSIN1 shRNA and HA-PACSIN1-rescue construct

Depositor

Richard Huganir

Article

Anggono et al Proc Natl Acad Sci U S A. 2013 Aug 20;11

Inserts

PACSIN1 (Pacsin1 Mouse)
H1-PACSIN1 shRNA cassette

Use

RNAi

Tags

HA

Expression

Mammalian

Mutation

5 silent mutations around shRNA recognition seque…

Promoter

CMV and H1

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-E2F3 3'UTR

Plasmid

#29470

Depositor

Judy Lieberman

Article

Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

Insert

E2F3 3'UTR (E2F3 Human)

Expression

Mammalian

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
NLS-tdPCP-CIBN

Plasmid

#183937

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to optogenetic CIBN domain; bound by PHR upon blue light exposure

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-CIBN (CIB1 Mustard Weed, Synthetic)

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(deltaC22, G120A)

Plasmid

#45449

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120, Glycine at position 12…

Available Since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-hu-c-Myc-dN2

Plasmid

#50774

Purpose

retroviral expression of human c-Myc dN2 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc -dN2 (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 42-439

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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1068 pGL3 FasL promoter (no SV40 prom)

Plasmid

#9029

Depositor

William Sellers

Article

Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

Insert

Fas ligand promoter (FASLG Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Removed the SV40 promoter from pGL3

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pTER GAMMA-TUBULIN shRNA Human

Plasmid

#87955

Purpose

reduced the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson, Ana Carrera

Article

Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

Insert

sh gamma-tubulin (TUBG1 Human)

Tags

No-tag

Expression

Mammalian

Mutation

The protein is a sh-gamma-tubulin resistant gene.…

Available Since

Oct. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-hu-c-Myc-dN1

Plasmid

#50773

Purpose

retroviral expression of human c-Myc dN1 fragment

Depositor

Shinya Yamanaka

Article

Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

c-Myc -dN1 (MYC Human)

Use

Retroviral

Expression

Mammalian

Mutation

N-terminal deletion, contains aa 14-439

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-C

Plasmid

#128417

Purpose

Express INTS3 C-termini (514-1042 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

INTS3 C-terminal region (514-1042 aa)

Promoter

Tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(92Q)-S776D

Plasmid

#21757

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 92 Q(Glutamines) and…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
integrin beta6 777T pcDNA1 neo

Plasmid

#13582

Depositor

Dean Sheppard

Article

Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

Insert

integrin beta 6 777T (ITGB6 Human)

Expression

Mammalian

Mutation

777T. Lacks last 11 amino acids of cytoplasmic do…

Available Since

Jan. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A FLAG-TEV-DPP9S S730A

Plasmid

#166801

Purpose

Mammalian cell expression vector for FLAG-tagged DPP9S. The S730A mutant is catalytically dead.

Depositor

Hao Wu

Article

Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

Insert

DPP9 (DPP9 Human)

Tags

FLAG-TEV

Expression

Mammalian

Mutation

S730A

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GSG1l-GFP KI

Plasmid

#131492

Purpose

Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gsg1l Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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LIC-Z-YFP

Plasmid

#153544

Purpose

perform light induced optogenetic clustering of TCR ζ-Chain tagged with yellow fluorescent protein as described in the associated publication

Depositor

Katharina Gaus

Article

Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

Insert

human TCR ζ-Chain (CD247 Human)

Tags

Venus YFP

Expression

Mammalian

Mutation

with mCherry in LIC-Z replaced by YFP

Promoter

CMV

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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PIK3CD gRNA (BRDN0001146545)

Plasmid

#76165

Purpose

3rd generation lentiviral gRNA plasmid targeting human PIK3CD

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PIK3CD (PIK3CD Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT-TODestFLAGHA_RBPMS

Plasmid

#59388

Purpose

Destination vector with RBPMS for stable cell line generation

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(30Q)-S776D

Plasmid

#21755

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only