We narrowed to 5,965 results for: pCas

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pLKO1-puro-U6-sgRNA-PLXNB2

Plasmid

#69240

Purpose

U6 driven SpCas9 sgRNA expression for PLXNB2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

PLXNB2 sgRNA (PLXNB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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JW776

Plasmid

#64817

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

N-terminal domain Firefly

Mutation

rCUC2

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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JW777

Plasmid

#64818

Purpose

BiFC CUC3

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC3 (CUC3 Mustard Weed)

Tags

C-terminal domain Firefly

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pIR197

Plasmid

#64819

Purpose

BiFC rCUC2

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Tags

C-terminal domain Firefly

Mutation

rCUC2

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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hs-FL-Kek1-myc2

Plasmid

#41170

Depositor

Norbert Perrimon

Article

Ghiglione et al Cell. 1999 Mar 19;96(6):847-56.

Insert

Kek1 (kek1 Fly)

Tags

2X myc

Expression

Insect

Promoter

Hsp70

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf G621S

Plasmid

#39769

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

G621S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf C295S

Plasmid

#39768

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

C295S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf F290I

Plasmid

#39766

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

F290I

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+S620A

Plasmid

#39765

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

R217L and S620A

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
D-raf R217L+C295S

Plasmid

#39764

Depositor

Norbert Perrimon

Article

Lu et al EMBO J. 1994 Jun 1;13(11):2592-9.

Insert

d-raf (Raf Fly)

Expression

Insect

Mutation

R217L and C295S

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pSpCTRE-CD4

Plasmid

#114010

Purpose

Dox-inducible Cas9 expression with a CD4 selection marker

Depositor

Charles Rudin

Article

Hulton et al Nat Cancer. 2020 Mar;1(3):359-369. doi:

Insert

SpCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-E

Plasmid

#78852

Purpose

Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

Depositor

Phillip Abbosh

Article

Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Cas9-P2A-Puro

Expression

Mammalian

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pHis-parallel1-NLSH2BCas9

Plasmid

#112065

Purpose

Expression and purification of NLSH2BCas9 for fungal gene editing

Depositor

Jeff Coleman

Article

Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

Insert

NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger

Use

CRISPR

Tags

His tag

Expression

Bacterial

Mutation

Cas9 was codon optimized for Aspergillus niger

Promoter

promoter in vector

Available Since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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p35S:Citrine-TurboID-3xHA

Plasmid

#199243

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID

Tags

3xHA and TurboID-3xHA

Expression

Plant

Promoter

CaMV 35S

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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ABE9

Plasmid

#194208

Purpose

Mammalian expression of ABE9

Depositor

Dali Li

Article

Chen et al Nat Chem Biol. 2022 Oct 13. pii: 10.1038

Insert

TadA-8e (N108Q/L145T)-nSpCas9

Use

CRISPR

Tags

6*His

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBBR-Cas9ΔyqhD-HA500

Plasmid

#220369

Purpose

A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

Depositor

Yilan Liu

Article

Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

Inserts

SpCas9
sgRNA for yqhD in P. putida KT2440

Use

CRISPR; Broad host range

Expression

Bacterial

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti_PE7-2A-BSD

Plasmid

#234073

Purpose

For lentiviral expression of PE7 with blasticidin resistance

Depositor

Greg Findlay

Article

Herger et al Cell Genom. 2025 Mar 18:100814. doi: 10.

Insert

PE7

Use

CRISPR and Lentiviral

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mammalian

Promoter

EF-1α core promoter

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCRISPR-cBEST

Plasmid

#125689

Purpose

C to T base editor for actinomycetes

Depositor

Tilmann Weber

Article

Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

Insert

streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available Since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-Hsp70-Cas9

Plasmid

#46294

Purpose

A codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter.

Depositor

Melissa Harrison, Kate O'Connor-Giles, Jill Wildonger

Article

Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

Insert

codon optimized Cas9

Use

CRISPR

Tags

3x Flag

Expression

Insect

Mutation

Silent change from C to A at bp 834 of insert

Promoter

Hsp70

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pX330-CENPA

Plasmid

#227282

Purpose

Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of CENPA (CENPA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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