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pET28-6xhis-sumo-CeMIGA(106-496)

Plasmid

#192832

Purpose

Express C.elegans MIGA fragment 106-496 in bacteria

Depositor

Karin Reinisch

Article

Hong et al J Cell Biol. 2022 Dec 5;221(12):e2022070

Insert

Mitoguardin homologue (miga-1 Nematode)

Tags

6xhis sumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlga

Plasmid

#168199

Purpose

Express zebrafish Kitlga, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Expression

Mammalian

Mutation

F198L - please see depositor comments

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pHA848 - mec-4p::hrp-1HsLCWTmScarlet

Plasmid

#139202

Purpose

Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

Plasmid

#139205

Purpose

Expresses mScarlet tagged hrp-1HsLCWT

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, last exon rep…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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Myo 9 Avi+Flag

Plasmid

#134969

Purpose

Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

Depositor

Martin Bähler

Article

Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

Insert

Myo 9 (hum-7 Nematode)

Tags

Avi and Flag

Expression

Insect

Promoter

Polyhedrin

Available Since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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TU#1805_CRISPR_unc-73_exon2

Plasmid

#82359

Purpose

to create unc-73B null allele

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

Cas9 and sgRNA against unc-73 exon2 (unc-73 Nematode)

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pDMG10b

Plasmid

#33186

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

aex-4/tag-81 3'UTR (tag-81, aex-4 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-142 site: aCacTaC

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
phRL-TKlk-lin-41_LCS-sm

Plasmid

#26487

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

lin-41_LCS-sm

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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phRL-TKlk-lin-41_LCS-2U

Plasmid

#26543

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

lin-41_LCS-2U

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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phRL-TKlk-lin-41_LCS-2G

Plasmid

#26544

Depositor

Chang-Zheng Chen

Article

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

lin-41_LCS-2G

Expression

Mammalian

Mutation

Seed regions of both target sites are mutated.

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-IgK-HT9-PDGFR-IRES-oScarlet-WPRE

Plasmid

#252087

Purpose

Expresses membrane bound halotag in mammalian cells with oScarlet fluorophore

Depositor

Karl Deisseroth

Article

Zhao et al ChemRxiv. 2024

Insert

IgK-HT9-PDGFR-IRES-oScarlet

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Igk-HT9-PDGFR-IRES-sfGFP-WPRE

Plasmid

#252088

Purpose

Expresses membrane bound halotag in mammalian cells with GFP fluorophore

Depositor

Karl Deisseroth

Article

Zhao et al ChemRxiv. 2024

Insert

Igk-HT9-PDGFR-IRES-sfGFP

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-Igk-HT9-PDGFR-IRES-BFP-WPRE

Plasmid

#252086

Purpose

Expresses membrane bound halotag in mammalian cells with BFP fluorophore

Depositor

Karl Deisseroth

Article

Zhao et al ChemRxiv. 2024

Insert

Igk-HT9-PDGFR-IRES-BFP

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-V5His6_R

Plasmid

#147400

Purpose

Insect Expression of CeAIN2_254-400

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-CeMextli_471-507opt-I497DI504D-GB1_AB

Plasmid

#148352

Purpose

Bacterial Expression of CeMextli_471-507opt-I497DI504D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-I497DI504D (Y18D10A.8 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-CeMextli_471-507opt-M493D-GB1_AB

Plasmid

#148353

Purpose

Bacterial Expression of CeMextli_471-507opt-M493D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-M493D (Y18D10A.8 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-CeMextli_471-507opt-Y479AL484AM485A-GB1_AB

Plasmid

#148354

Purpose

Bacterial Expression of CeMextli_471-507opt-Y479AL484AM485A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

CeMextli_471-507opt-Y479AL484AM485A (Y18D10A.8 Nematode)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-CeAIN2_254-400-V5His6_R

Plasmid

#147407

Purpose

Insect Expression of CeAIN2_254-400

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

Plasmid

#139203

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

Plasmid

#139206

Purpose

Expresses mScarlet tagged hrp-1HsLCD290V

Depositor

Anne Hart

Article

Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

Insert

hrp-1 (hrp-1 Nematode)

Expression

Worm

Mutation

mScarlet added between RRMs and LC, D290V, last e…

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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