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pCS2+5'MT-wbp2nl

Plasmid

#172467

Purpose

expresses myc-tagged version of wild-type protein

Depositor

Sally Moody

Article

Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

Insert

wbp2nl

Use

Xenopus expression

Tags

Myc

Promoter

SP6

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pIVT-osTIR1-9myc

Plasmid

#182043

Purpose

in vitro transcription of osTIR1

Depositor

Janice Evans

Article

Camlin et al Biol Reprod. 2019 Oct 25;101(4):704-718.

Insert

osTIR1

Use

In vitro transcription

Tags

myc

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

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pT-35

Plasmid

#16092

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

SMT3 (SMT3 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pMSCV Cyclin D2-short

Plasmid

#21655

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV Cyclin D2 long-wt

Plasmid

#21646

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Cyclin D2 (CCND2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLK25

Plasmid

#37255

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

Scribble (SCRIB Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 727-1630

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
NK66

Plasmid

#176606

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

Syk-BFP (Syk Mouse, Synthetic)

Use

Lentiviral

Expression

Mammalian

Mutation

n/a

Available Since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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TNXXL-CTS68

Plasmid

#48343

Purpose

FRET reporter for Ca2+ inside primary cilia

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

CTS68

Tags

TN-XXL

Expression

Mammalian

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pSico Spry-2

Plasmid

#14809

Depositor

Tyler Jacks

Article

Shaw et al Genes Dev. 2007 Mar 15. 21(6):694-707.

Insert

Sprouty-2 shRNA (Spry2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

April 5, 2007

Availability

Academic Institutions and Nonprofits only
PALI1_pHIVEGFP

Plasmid

#191252

Purpose

Expresses PALI1 in mammalian cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

NA

Promoter

EF1alpha

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN-ΔN

Plasmid

#32792

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 1-63

Available Since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pCHA1.1-Rap2a-V5

Plasmid

#149509

Purpose

Stable expression of human Rap2a.

Depositor

David Sabatini

Article

Adelmann et al Nature. 2020 Nov 18. pii: 10.1038/s41586

Insert

RAP2 (RAP2A Human)

Use

Lentiviral

Tags

V5

Mutation

Codon Optimized Sequence

Promoter

Ubc

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET28 Set9 mutant

Plasmid

#24083

Depositor

Danny Reinberg

Article

Nishioka et al Genes Dev. 2002 Feb 15. 16(4):479-89.

Insert

Set9 (SETD7 Human)

Tags

FLAG and His

Expression

Bacterial

Mutation

H297A

Available Since

Oct. 27, 2010

Availability

Academic Institutions and Nonprofits only
mCherryC1-PIPKI{gamma}661

Plasmid

#29584

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Tags

mCherry

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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pLK67

Plasmid

#37251

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

Scribble (SCRIB Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-728

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/HisC 14-3-3 epsilon K49E

Plasmid

#11943

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Tags

His

Expression

Mammalian

Mutation

K49E

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pENTR APLP2 shRNA 3

Plasmid

#30161

Depositor

Dennis Selkoe, Tracy Young-Pearse

Article

Young-Pearse et al Neural Dev. 2008 Jun 23;3:15. doi: 10.11

Insert

APLP2 shRNA 3

Expression

Mammalian

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pCG-A-sacB(SP)-B

Plasmid

#87370

Purpose

An ORF part-containing vector harbouring the signal peptide from SacB with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

Depositor

Tom Ellis

Article

Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

Insert

SacB signal peptide

Use

Synthetic Biology

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pIF251

Plasmid

#133935

Purpose

Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.

Depositor

IIya Finkelstein

Article

Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.

Insert

Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda

Use

Λ-phage recombineering plasmid

Available Since

March 3, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAct-dCBEevoAPOBEC1

Plasmid

#195514

Purpose

Ubiquitous expression of dCBEevoAPOBEC1 in Drosophila

Depositor

Michael Boutros

Article

Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

Insert

dCBE-evoAPOBEC1

Use

CRISPR

Expression

Insect

Promoter

act5C

Available Since

Sept. 12, 2023

Availability

Academic Institutions and Nonprofits only

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