We narrowed to 16,162 results for: grna

1. lentiCRISPR - Amplicon, BCR/ABL sgRNA 2

   Plasmid

   #70658

   Purpose

   Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an intergenic BCR/ABL-targeting sgRNA element from U6 promoter. Lentiviral backbone.

   Depositor

   David Sabatini

   Article

   Wang et al Science. 2015 Oct 15. pii: aac7041.

   Insert

   sgRNA against BCR/ABLamplicon found in CML-derived K562 cell line
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pdCas9+sgRNA expression vector precursor

   Plasmid

   #171798

   Purpose

   PICASSO: Library expression vector precursor for paired dCas9+sgRNA expression

   Depositor

   Stephen Elledge

   Article

   Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pLtetO

   Available Since

   Aug. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLG1-puro Non-targeting sgRNA 3

   Plasmid

   #109003

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   Non-targeting sgRNA #3
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6 promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNA

   Plasmid

   #187068

   Purpose

   Gp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutation

   Depositor

   Renzhi Han

   Article

   Xu et al Nat Commun. 2021 Jun 17;12(1):3719. doi:

   Insert

   Gp41-ABEmaxC2‐NG
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   MHP1

   Available Since

   Aug. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-pMecp2-SaCas9-pU6-sgRNA

   Plasmid

   #159173

   Purpose

   Vector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neurons

   Depositor

   Chung Tin

   Article

   Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

   Insert

   SaCas9
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   pMecp2

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

   Plasmid

   #162335

   Purpose

   Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. non-targeting control gRNA (BRDN0001146145)

   Plasmid

   #80198

   Purpose

   3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   Non-targeting control gRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 30, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL1 sgRNA(MS2)-MS2-AIDmono

   Plasmid

   #112127

   Purpose

   empty sgRNA cloning vector with MS2-AIDmono

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   AID mono mutant (AICDA Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   N- mutated and C- terminal truncated, H130A/R131E…

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

   Plasmid

   #159283

   Purpose

   A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Human INS sgRNA
   Human INS guide RNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. non-targeting control gRNA (BRDN0001145598)

    Plasmid

    #80173

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLH-sgRNA1-boxB-MS2-PP7

    Plasmid

    #75397

    Purpose

    sgRNA1-boxB-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-boxB-MS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-EGFR-L858R-nick sgRNA

    Plasmid

    #214097

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce EGFR L858R mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R nicking sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti-EGFR-T790M-nick sgRNA

    Plasmid

    #214098

    Purpose

    Lentiviral vector expressing nicking sgRNA to induce EGFR T790M mutation using PE3

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR T790M nicking sgRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

    Plasmid

    #85714

    Purpose

    Telomere-targeting Nme sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    telomere sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC1812_pCR8-gCasRx-CUG (control gRNA)

    Plasmid

    #118645

    Purpose

    Transient transfection; Expressed Control (CUG repeat) CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gCasRx-CUG
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-sgRNA(MS2)-puro-barcode

    Plasmid

    #89493

    Purpose

    Cloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seq

    Depositor

    Gary Hon

    Article

    Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

    Insert

    sgRNA (with MS2 loop)
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    June 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-SMVP-Cas9N-U6-gRNA TdR

    Plasmid

    #80940

    Purpose

    Expresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.

    Depositor

    George Church

    Article

    Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

    Insert

    Cas9N
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. non-targeting control gRNA (BRDN0001148500)

    Plasmid

    #80260

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pShCAST(Cas12k-TnsC)-sgRNA_entry (CJT28)

    Plasmid

    #181789

    Purpose

    Expresses 3-component ShCAST containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2AmCherry-W

    Plasmid

    #163179

    Purpose

    Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of mCherry tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    YAP1 (YAP1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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