We narrowed to 6,252 results for: nls

1. AA058

   Plasmid

   #216040

   Purpose

   Fragmid fragment: (C' terminus) for prime editing

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   MMLV-RT_v1.1; SV40NLS_v1.3
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. LbCas12a-niCPE

   Plasmid

   #213747

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-nLbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A in LbCas12a

   Promoter

   CMV

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRRL-mU6-GFPGO-IRES-mScarletI-PGK-Neo

   Plasmid

   #136897

   Purpose

   Lentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   GFPGO-IRES-mScarletI
   

   Use

   Lentiviral

   Tags

   3xNLS on mScarlet

   Mutation

   ATG>ACG

   Promoter

   SFFV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

   Plasmid

   #84245

   Purpose

   Expresses direct fusion KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   KRAB-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, KRAB, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MMLV-RT(dRH) for Split-PE (pEH50)

   Plasmid

   #190106

   Purpose

   Expresses MMLV-RT (from PE2) with truncated RNAse H domain (dRH) for Split-PE use

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   MMLV-RT(delta RNAseH)
   

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   mutations from RT in PE2 and truncation of RNAse …

   Promoter

   CMV

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYN2_1

   Plasmid

   #184757

   Purpose

   Plasmid for genome editing by CRISPR/Cas9

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   Cas9
   sgRNA scaffold where sfGFP is replaced with gRNA protospacer of interest, which will be proceeded by the HDV Ribozyme
   

   Use

   Synthetic Biology

   Tags

   2xNLS

   Expression

   Bacterial and Yeast

   Promoter

   PGK1 and tRNA-Phe-HDV Ribozyme

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pH-mono-MAD7

   Plasmid

   #170134

   Purpose

   For plant prime editing in rice plants or monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al J Genet Genomics. 2021 May 3. pii: S1673

   Insert

   MAD7(ErCas12a)
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   maize Ubiquitin-1, switchgrass Ubiquitin-1

   Available Since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCH68 (dAsCas12a-3xKRAB piggyBac)

   Plasmid

   #217334

   Purpose

   piggyBac expression of dAsCas12a-3xKRAB

   Depositor

   Luke Gilbert

   Article

   Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

   Insert

   dAsCas12a
   

   Use

   CRISPR

   Tags

   nucleoNLS-3xKRAB-3xHA-P2A-TagBFP2

   Expression

   Mammalian

   Mutation

   E993A

   Promoter

   CAG

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

   Plasmid

   #84247

   Purpose

   Expresses direct fusion VPR-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   VPR-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, VPR, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. LbCas12a-nuCPE

    Plasmid

    #213748

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-LbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R in LbCas12a

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. attB53-Pac-TRE-mCherry-attB53

    Plasmid

    #183611

    Purpose

    RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    TRE-mCherry
    

    Use

    Rmce

    Promoter

    TRE

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AA011

    Plasmid

    #215973

    Purpose

    Fragmid fragment: (N' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; rAPOBEC1_v1.1; Linker_v1.1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AA010

    Plasmid

    #216038

    Purpose

    Fragmid fragment: (C' terminus) for C>T base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    UGI_v1.1; NpNLS_v1.1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AA013

    Plasmid

    #215975

    Purpose

    Fragmid fragment: (N' terminus) for A>G base editing

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; BPNLS_v1.1; ABE8e_v1.1; Linker_v1.1
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MMLV-RT for Split-PE (pEH45)

    Plasmid

    #190105

    Purpose

    Expresses MMLV-RT (full-length, from PE2) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    MMLV-RT
    

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    RT pentamutant from PE2 (D200N, T306K, W313F, T33…

    Promoter

    CMV

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJ23119-sgRNA

    Plasmid

    #113654

    Purpose

    sgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.

    Depositor

    Jeffrey Barrick

    Article

    Geng et al ACS Synth Biol. 2019 Mar 15;8(3):521-531

    Insert

    promoter J23119 and sgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. sABE v3.22 Lenti_1

    Plasmid

    #209555

    Purpose

    pCMV-SV40NLS-2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN

    Depositor

    Xue Gao

    Article

    Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

    Insert

    2xFKBP3-TadA-8e(77-167)-SpCas9(D10A, 2-468)-gp41-1_IntN
    

    Expression

    Mammalian

    Promoter

    pCMV

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. AA295

    Plasmid

    #215986

    Purpose

    Fragmid fragment: (N' terminus) SV40 Nuclear Localization Signal

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    Start; SV40NLS_v1.19
    

    Use

    Fragment

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78306

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADfluw-Hsp70
    

    Available Since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

    Plasmid

    #78307

    Purpose

    Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

    Depositor

    Benjamin White

    Article

    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

    Insert

    T2A-nlsLexA::GADflluw
    

    Available Since

    July 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge