We narrowed to 15,943 results for: grna

1. pBBK11 pCAS-Tyr-[gRNA: 1=FF18] (SplitKanR, AmpR)

   Plasmid

   #178995

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-RSV-GFP-U6-CCR5 gRNA (SpyCas9 scaffold)

   Plasmid

   #113041

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CCR5-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA CCR5 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

   Plasmid

   #113697

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-RSV-GFP-U6-EMX1 gRNA (SpyCas9 scaffold)

   Plasmid

   #113040

   Purpose

   AAV vector; encodes GFP as well as a U6-driven EMX1-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   gRNA EMX1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB-U6-Chrna7-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128342

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna7 (Chrna7 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-GFP-U6-CFTR gRNA (SpyCas9 scaffold)

   Plasmid

   #113042

   Purpose

   AAV vector; encodes GFP as well as a U6-driven CFTR-targeting gRNA (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CFTR gRNA (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFB-U6-Chrna3-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128338

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna3 (Chrna3 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFB-U6-Chrna2-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128337

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna2 (Chrna2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFB-U6-Chrna4-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128339

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrna4 (Chrna4 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFB-U6-Chrnb3-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128344

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrnb3 (Chrnb3 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA-Dual_filler(hU6_H1)-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #239608

    Purpose

    Backbone for the cloning of a dual sgRNA expression plasmid (hU6 and H1 promoters) using BsmbI. Selectable with a G418 resistance

    Depositor

    Philipp Rathert

    Article

    Stadager et al Cell Rep Methods. 2025 Jun 16;5(6):10107

    Insert

    filler-trcr-H1-filler
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid

    #234724

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.U6-sasgRNA(SapI)_Ple155-CI-EGFP-W3SL_BbsI(GGA)

    Plasmid

    #231367

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV.U6-sasgRNA(SapI)_Ple155-CI-mRuby2-W3SL_BbsI(GGA)

    Plasmid

    #231368

    Purpose

    Ple155-driven mRuby2, also encoding U6-driven sgRNA cassette, with SapI sites to clone crRNA sequence

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    mRuby2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. 1203U-U6-gRNA-pCMV-wtCas9-NG-P2A-mcherry-3'dmDR

    Plasmid

    #221448

    Purpose

    Expresses wtCas9-NG with double processed direct repeats (15nt) and a non-target spacer in between at 3' end, mCherry tag, and the gRNA targeting HEK293T-site3

    Depositor

    Omar Akbari

    Inserts

    wtCas9-NG
    mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 783-Rx-mU6: RfxCas13d gRNA and array cloning backbone

    Plasmid

    #228361

    Purpose

    mU6-driven expression of RfxCas13d gRNAs and arrays. Contains AarI sites for guide cloning.

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Rfxcas13d grna expression …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p104Tol2-tp1:Cas9-t2A-GFP, 4xU6:sgRNA

    Plasmid

    #227773

    Purpose

    Expression of tp1 (Notch) dependent Cas9-GFP and U6-driven 4 sgRNAs in zebrafish

    Depositor

    Bushra Raj

    Article

    Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

    Inserts

    Cas9-t2A-GFP
    4xU6:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6 and tp1

    Available since

    Dec. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

    Plasmid

    #209923

    Purpose

    A knock-out vector for the dog CTNND1

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    A gRNA targeting the dog CTNND1 gene.
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX552-EF1a-DIO Chronos-eGFP(with gRNA scaffold)

    Plasmid

    #199582

    Purpose

    Expresses Chronos-eGFP in a Cre-dependent manner

    Depositor

    Bryan Copits

    Article

    Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

    Insert

    Chronos eGFP
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a intron A

    Available since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV-hSyn-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid

    #177360

    Purpose

    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression from Synapsin promoter

    Depositor

    Naoki Yamamoto

    Article

    Yamamoto et al Research Square [Preprint] 2021. doi: 10

    Inserts

    tdTomato
    Chimeric guide for SaCas9
    

    Use

    AAV

    Tags

    P2A and a tetracycline-controlled transactivator …

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsine promoter and U6 promoter

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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