We narrowed to 16,185 results for: grna

1. pEG302 22aa SunTag VP64 gRNA17 (FWA)

   Plasmid

   #120250

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g17_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP

   Plasmid

   #113155

   Purpose

   An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat TH
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   mU6 and hU6

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP

   Plasmid

   #113157

   Purpose

   An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   Two gRNAs for rat MANF
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Promoter

   mU6 and hU6

   Available Since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-2/3-3'

   Plasmid

   #117325

   Purpose

   CRISPR-Cas9 for miR-124-2/3, 3'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-2/3-3' (MIR124-3 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1-3'

   Plasmid

   #117324

   Purpose

   CRISPR-Cas9 for miR-124-1, 3'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-1-3' (MIR124-1 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-3-5'

   Plasmid

   #117323

   Purpose

   CRISPR-Cas9 for miR-124-3, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-3-5' (MIR124-3 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

   Plasmid

   #117322

   Purpose

   CRISPR-Cas9 for miR-124-1/2, 5'

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   gRNA miR-124-1/2-5' (MIR124-1 Synthetic)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL5 sgRNA(MS2)-MS2-hA3a D131A

   Plasmid

   #112131

   Purpose

   empty sgRNA cloning vector with MS2-humanA3a D131A

   Depositor

   Fei-Long Meng

   Article

   Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

   Insert

   human Apobec3a D131A mutant (APOBEC3A Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D131A mutant in hA3a

   Promoter

   hU6,CBh

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

   Plasmid

   #172982

   Purpose

   control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   control sgRNA
   

   Use

   CRISPR and Lentiviral

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEM040 [Multiome Perturb-seq sgRNA expression vector]

    Plasmid

    #224898

    Purpose

    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA, engineered for high-efficiency ID assignment in Multiome Perturb-seq experiments

    Depositor

    Thomas Norman

    Article

    Metzner et al Cell Syst. 2025 Jan 15;16(1):101161. doi

    Inserts

    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA

    Plasmid

    #113691

    Purpose

    U6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.

    Depositor

    Jonathan Ploski

    Article

    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

    Insert

    SaCas9
    

    Use

    AAV, CRISPR, and Cre/Lox

    Tags

    NLS

    Promoter

    Cytomegalo Virus(CMV)

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

    Plasmid

    #211687

    Purpose

    U6-sgRNA-EFS-Cas9-P2A-Puro

    Depositor

    Christof Fellmann

    Article

    Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

    Insert

    SpyCas9 and guide RNA recipient vector
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

    Plasmid

    #221233

    Purpose

    Express sgRNA targeting HEK3 loci with nCas9(D10A)

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-P2A-EGFP
    

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-U6-Agrp-(mouse)-MS2gRNA-hSyn-MCP-MSN

    Plasmid

    #210709

    Purpose

    This Plasmid express U6 promoter driven SpdCas9 specific gRNA and hSyn promoter driven MCP-MSN transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    SpdCas9 specific Agrp (mouse) MS2gRNA and MCP-MSN
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

    Plasmid

    #207880

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid

    #234724

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    T2A-GFP

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

    Plasmid

    #179002

    Purpose

    Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PGK-Hygro-amp-sgRNA-resistant PBRM1

    Plasmid

    #107407

    Purpose

    Lentiviral expression of gRNA resistant PBRM1

    Depositor

    William Kaelin

    Article

    Gao et al Proc Natl Acad Sci U S A. 2017 Jan 31;11

    Insert

    sg resistant PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Mutation

    sgRNA resistant

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEM318 - sgRNA mosTI unc-119 single-copy

    Plasmid

    #159822

    Purpose

    Single copy or array insertion by MosTI using split unc-119 selection

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Insert

    sgRNA mosTI unc-119 single-copy
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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