Addgene

We narrowed to 1,787 results for: rpe

Showing: 41 - 60 of 1787 results

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pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

Plasmid

#84275

Purpose

pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

GluN2B

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

CMV

Available since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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CLYBL hOPM

Plasmid

#112499

Purpose

Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locus

Depositor

Bruce Conklin

Insert

OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS hOPM

Plasmid

#112498

Purpose

Homologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.

Depositor

Bruce Conklin

Insert

OTX2, PAX6, MITF (PAX6, MITF, OTX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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CAG-mCherry

Plasmid

#108685

Purpose

Strong CAG promoter with mCherry expression for transfection efficacy screening

Depositor

Jordan Green

Article

Mishra et al Regen Eng Transl Med. 2019 Sep;6(3):273-

Insert

mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pInt-ERCreER

Plasmid

#190040

Purpose

This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

Depositor

Kayoko Tanaka

Article

Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

Insert

ERT2-Cre-ERT2 flanked by AAVS1

Use

CRISPR and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO(Neo)-Venus-Flag-WT wobbled cdc20 (1991)

Plasmid

#61848

Purpose

for making stable RPE cells expressing WT cdc20

Depositor

Jonathon Pines

Insert

cdc20 (CDC20 Human)

Use

Tags

Flag and Venus

Expression

Mammalian

Mutation

wobbled

Promoter

CMV

Available since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only
KSHV Tiling Library

Pooled Library

#180272

Purpose

sgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.

Depositor

Britt Glaunsinger

Article

Morgens et al PLoS Pathog. 2022 Jan 18;18(1):e1010236.

Expression

Mammalian

Species

Kaposi’s sarcoma-associated herpes virus

Use

Lentiviral

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only
E. coli MEV20

Bacterial Strain

#197113

Purpose

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
pDA095

Plasmid

#131391

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pDA093

Plasmid

#131390

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCYP37

Plasmid

#212870

Purpose

Expresses the Nocardia terpenica LRD-modifying cytochrome P450 enzyme

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Insert

cytochrome P450

Use

Tags

Expression

Bacterial

Mutation

Promoter

PcymRC

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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TP53 MITE Library

Pooled Library

#113569

Purpose

Pooled lentiviral plasmid library containing ~8,000 single amino acid substitution and nonsense mutant variants of p53

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Expression

Mammalian

Species

Homo sapiens

Use

Lentiviral

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
pCDNA4.TO-K5-2xCSTREP

Plasmid

#136154

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF50-2xCSTREP

Plasmid

#136211

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF50

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF50

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF8-2xCSTREP

Plasmid

#136172

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF8

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF8

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF22-2xCSTREP

Plasmid

#136182

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF22

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF22

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF47-2xCSTREP

Plasmid

#136208

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF47

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF47

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-K8.1-2xCSTREP

Plasmid

#136157

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K8.1

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

K8.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO-ORF26-2xCSTREP

Plasmid

#136186

Purpose

Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF26

Depositor

Britt Glaunsinger

Article

Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

Insert

ORF26

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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