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pDONR223-PAK4

Plasmid

#23713

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PAK4 (PAK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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NGFR P210

Plasmid

#27486

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

bcr/abl (P210) (ABL1, BCR Human)

Use

Retroviral

Tags

IRES and NFGR

Expression

Mammalian

Mutation

Contains the "complete" bcr/abl fusion

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLT4

Plasmid

#116745

Purpose

Lentiviral expression of FLT4

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

FLT4 (FLT4 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223-PRKCB2

Plasmid

#23764

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKCB2 (PRKCB Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-AAK1

Plasmid

#23844

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AAK1 (AAK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-NEK9

Plasmid

#23459

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NEK9 (NEK9 Human)

Use

Gateway Cloning

Mutation

1MM

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-RPS6KA5

Plasmid

#23758

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

RPS6KA5 (RPS6KA5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCS2-CIB1-mCerulean-Rab11

Plasmid

#140574

Purpose

For use in light-induced protein inactivation through the interaction with CRY2

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Inserts

CIB1 (CIB1 Mustard Weed)
Rab11A (RAB11A Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-NME6

Plasmid

#23562

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NME6 (NME6 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-WNK1

Plasmid

#23670

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

WNK1 (WNK1 Human)

Use

Gateway Cloning

Mutation

G4C

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-PRKAR1B

Plasmid

#23376

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAR1B (PRKAR1B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-STK11

Plasmid

#23802

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

STK11 (STK11 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pDONR223-PIK3R5

Plasmid

#23819

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PIK3R5 (PIK3R5 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv1-sgPHGDH-G5

Plasmid

#83913

Purpose

stable knockout

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

Phosphoglycerate dehydrogenase (PHGDH Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pDONR223-TRIB3

Plasmid

#23677

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

TRIB3 (TRIB3 Human)

Use

Gateway Cloning

Mutation

P5T

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223_CHD2_p.P195L

Plasmid

#81463

Purpose

Gateway Donor vector containing CHD2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway Cloning

Mutation

P195L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHD2_p.E287Q

Plasmid

#81467

Purpose

Gateway Donor vector containing , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway Cloning

Mutation

E287Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CHD2_p.E412K

Plasmid

#81469

Purpose

Gateway Donor vector containing CHD2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CHD2 (CHD2 Human)

Use

Gateway Cloning

Mutation

E412K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-SpyTag-Flag-SRSF1-V5-mCherry

Plasmid

#235089

Purpose

SRSF1 mCherry fusion protein without MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-SpyTag-Flag-SRSF1-V5-mCherry (SRSF1 Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR223-PI4K2B

Plasmid

#23507

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PI4K2B (PI4K2B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only