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RNAPII_CTD27-52_K5S

Plasmid

#98679

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II with K5S

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

K1838S, K1859S, K1866S, K1873S, K1887S

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

Plasmid

#140200

Purpose

Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

dLbCas12a crRNA scaffold

Promoter

PgpdA

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T MDM2 S166D

Plasmid

#16238

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Nov . 3(11):973-82.

Insert

MDM2 (MDM2 Human)

Tags

GST

Expression

Bacterial

Mutation

Ser166 replaced by Asp

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pNAS1B split mCherry N-MESVD/TRAP(RK)-C

Plasmid

#61967

Purpose

Duet plasmid for weak split mCherry assembly (as compared to N-ME(pS)VD/TRAP(RK)-C). N-mCherry is fused to the peptide MESVD

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-MESVD
TRAP(RK)-C-mCherry

Expression

Bacterial

Promoter

PBAD and PLtetO

Available Since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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MEIS1B-pSP65

Plasmid

#21019

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 1997 Nov;17(11):6448-58

Insert

MEIS1B (Meis1 Mouse)

Use

In vitro tnt

Tags

Flag

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
MTK2_016

Plasmid

#123708

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-A

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HOXD12 (murine) pRSETa

Plasmid

#8554

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Tags

HIS and XPress

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
RNAPII_CTD38-52

Plasmid

#98685

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Article

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Mutation

heptads 38-52, residues 1853-1970

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSIN-Arkadia-gRNA2

Plasmid

#180362

Purpose

targeting mouse Arkadia gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Arkadia targeting gRNA (Rnf111 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

human U6

Available Since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pPuroR-MCS-GAL4-MiniWhite

Plasmid

#165890

Purpose

Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable Empty Enhancer Driver

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJH2037

Plasmid

#179220

Purpose

Psra-11::UNC-7-UrSL-wCherry unc-54 3' UTR C.elegans neural expression of UNC-7-wCherry

Depositor

Mei Zhen

Article

Kawano et al Neuron. 2011 Nov 17;72(4):572-86. doi: 1

Insert

UNC-7 (unc-7 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Psra-11

Available Since

Feb. 28, 2022

Availability

Academic Institutions and Nonprofits only

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MTK4b_004

Plasmid

#123843

Purpose

Encodes spacer sequence as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pET-CH2 (1192-1318)

Plasmid

#99338

Purpose

Bacterial expression of mouse CBP CH2 domain

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

CBP CH2 (1192-1318) (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmLSm4_A

Plasmid

#145883

Purpose

Insect Expression of DmLSm4

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmLSm4 (LSm-4 Fly)

Tags

V5-6xHis

Expression

Insect

Mutation

two silent mutations compared to the sequence of …

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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UASgal-A9-Pleum

Plasmid

#60343

Purpose

Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.

Depositor

Hal Alper

Article

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available Since

Nov. 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSG-3'Hu-RelA

Plasmid

#27276

Depositor

Thomas Gilmore

Article

Morin et al Nucleic Acids Res. 1993 May 11. 21(9):21

Insert

RelA (RELA Human)

Tags

GAL4 (aa1-147)

Expression

Mammalian

Mutation

Contains only the transactivation domain (aa 292-…

Available Since

Feb. 1, 2011

Availability

Academic Institutions and Nonprofits only
MTK2_017

Plasmid

#123709

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-B

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pCFJ914

Plasmid

#44489

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1 [Peft-3 GFP H2B tbb-2 UTR, NeoR]

Use

Gateway expression clone

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ABKAR-Kras

Plasmid

#84637

Purpose

Unimolecular, FRET-based AMPK/BRSK activity reporter; plasma membrane targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean3-FHA1-AMPKsub-cpVenus[E172]-Kras

Tags

6xHis, C-terminal targeting sequence from Kras, C…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pET

Plasmid

#8494

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Bacterial

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only