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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,562 results for: nls

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Showing: 6041 - 6060 of 6562 results

  1. Tad-CBE(CCT2)

    Plasmid
    #235071
    Purpose
    Expresses Tad-CBE(CCT2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(CCT2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235071-plasmid-map-sequence-id-466783

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  2. Tad-CBE(ACT4)

    Plasmid
    #235060
    Purpose
    Expresses Tad-CBE(ACT4) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACT4)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235060-plasmid-map-sequence-id-466786

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  3. Tad-CBE(ACC2)

    Plasmid
    #235061
    Purpose
    Expresses Tad-CBE(ACC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, SV40NLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235061-plasmid-map-sequence-id-466785

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  4. Tad-CBE(TCT1)

    Plasmid
    #235065
    Purpose
    Expresses Tad-CBE(TCT1) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCT1)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235065-plasmid-map-sequence-id-466798

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  5. Tad-CBE(TCC2)

    Plasmid
    #235067
    Purpose
    Expresses Tad-CBE(TCC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(TCC2)-SpCas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235067-plasmid-map-sequence-id-466795

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  7. T2A Split/Link TurboID Kit

    Plasmid kit
    #1000000249
    Purpose
    Modular assembly of expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Lentiviral, Mammalian Expression
    Cloning type
    Golden Gate
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only

  8. CEAS (C-SWAT for Essential Allele Strains)

    Plasmid kit
    #1000000223
    Purpose
    Vectors used to lower gene expression in S. cerevisiae. Compatible with the genome-wide S. cerevisiae C-SWAT strain collection.
    Depositor
    Rob Mitra
    Application
    Gene Expression and Labeling
    Vector type
    Yeast Expression
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. nCas9-NG_P2A-mCherry

    Plasmid
    #219934
    Purpose
    Mammalian expression of Sp-nCas9-NG with P2A-mCherry marker for use with MOBE systems
    Depositor
    Alexis Komor
    Publication
    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Sp-nCas9(D10A)-NG_P2A-mCherry
    Use
    CRISPR
    Tags
    SV40 bpNLS and SV40 bpNLS-P2A-mCherry
    Expression
    Mammalian
    Mutation
    nCas9(D10A)
    Promoter
    CMV
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219934-plasmid-map-sequence-id-435500

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  11. pJS145

    Plasmid
    #179159
    Purpose
    Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.
    Depositor
    Iva Greenwald
    Publication
    Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11
    Insert
    rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
    Expression
    Worm
    Mutation
    Flexon replacing 2nd intron of 2xNLS::GFP
    Promoter
    rps-27p
    Available since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    179159-plasmid-map-sequence-id-352801

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  12. SERCA

    Plasmid
    #42413
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SERCA (PSAT1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  13. pRGEN-CMV-VPR-WT CjCas9 V-WT

    Plasmid
    #169914
    Purpose
    Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.
    Depositor
    Zhili Rong
    Publication
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169914-plasmid-map-sequence-id-336053

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  14. pPK-351

    Plasmid
    #157921
    Purpose
    pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD
    Depositor
    Phillip Kyriakakis
    Publication
    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    PIF3-MTAD
    IRES
    PhyB(1-621)-SV40NLS-Gal4DBD
    Use
    Synthetic Biology; Optogenetics
    Tags
    HA tag and SV40NLS
    Expression
    Mammalian
    Mutation
    pif3 1-523
    Promoter
    CMV
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    157921-plasmid-map-sequence-id-311915

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  15. L4038

    Plasmid
    #1620
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    let858enh>>, let858enh<<, let858-455,…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1620-plasmid-map-sequence-id-230

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  16. L4041

    Plasmid
    #1622
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, SV40NLS, SV40NLS, SV40NLS, gfpN, I…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    1622-plasmid-map-sequence-id-232

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  17. L4054

    Plasmid
    #1632
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, SV40NLS, SV40NLS, SV40NLS, gfpN, I…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1632-plasmid-map-sequence-id-242

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  18. L4453

    Plasmid
    #1655
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    rps5-40k, rps55', SV40NLS, SV40NLS, SV40NLS,…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    1655-plasmid-map-sequence-id-265

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  19. L4686

    Plasmid
    #1673
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    myo3-2341, myo3Tsa, IVSA, SV40NLS, SV40NLS, SV40N…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1673-plasmid-map-sequence-id-283

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  20. L4019

    Plasmid
    #1615
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, rf2211, SV40NLS, SV40NLS, SV40NLS, SV40NLS,…
    Expression
    Worm
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1615-plasmid-map-sequence-id-225

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Showing: 6041 - 6060 of 6562 results